Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite

Autores
Katzer, Frank; Canton, German Jose; Burrels, Alison; Palarea-Albaladejo, Javier; Horton, Ben; Bartley, Paul M.; Pang, Yvonne; Chianini, Francesca; Innes, Elisabeth A.; Benavides-Silván, Julio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at significantly (p < 0.0001) lower levels in samples from the vaccinated/challenged group (0% in heart and 5.9% in skeletal muscles), when compared to the non-vaccinated/challenged animals (75% heart, 87.9% skeletal muscle). S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. Non-vaccinated/challenged animals showed more frequent lesions in muscles and central nervous system than the vaccinated animals. These results demonstrate that vaccination of lambs with the incomplete S48 T. gondii strain, can protect against establishment of tissue cysts following challenge with a complete strain of T. gondii. Consumption of undercooked meat containing T. gondii cysts is a major route of transmission to people, therefore vaccination of food animals may improve the safety of meat for human consumption.
EEA Balcarce
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Burrels, Alison. Moredun Research Institute; Reino Unido
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Horton, Ben. Moredun Research Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fuente
Veterinary Parasitology 205 (1–2) : 46-56 (September 2014)
Materia
Cordero
Inmunización
Toxoplasma gondii
Vacuna
Toxoplasmosis
Zoonosis
Lambs
Immunization
Vaccines
Zoonoses
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4372
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4372
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network_name_str INTA Digital (INTA)
spelling Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasiteKatzer, FrankCanton, German JoseBurrels, AlisonPalarea-Albaladejo, JavierHorton, BenBartley, Paul M.Pang, YvonneChianini, FrancescaInnes, Elisabeth A.Benavides-Silván, JulioCorderoInmunizaciónToxoplasma gondiiVacunaToxoplasmosisZoonosisLambsImmunizationVaccinesZoonosesThis study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at significantly (p < 0.0001) lower levels in samples from the vaccinated/challenged group (0% in heart and 5.9% in skeletal muscles), when compared to the non-vaccinated/challenged animals (75% heart, 87.9% skeletal muscle). S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. Non-vaccinated/challenged animals showed more frequent lesions in muscles and central nervous system than the vaccinated animals. These results demonstrate that vaccination of lambs with the incomplete S48 T. gondii strain, can protect against establishment of tissue cysts following challenge with a complete strain of T. gondii. Consumption of undercooked meat containing T. gondii cysts is a major route of transmission to people, therefore vaccination of food animals may improve the safety of meat for human consumption.EEA BalcarceFil: Katzer, Frank. Moredun Research Institute; Reino UnidoFil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Burrels, Alison. Moredun Research Institute; Reino UnidoFil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino UnidoFil: Horton, Ben. Moredun Research Institute; Reino UnidoFil: Bartley, Paul M. Moredun Research Institute; Reino UnidoFil: Pang, Yvonne. Moredun Research Institute; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoFil: Innes, Elisabeth A. Moredun Research Institute; Reino UnidoFil: Benavides-Silván, Julio. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; EspañaElsevier2019-02-01T12:16:51Z2019-02-01T12:16:51Z2014-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0304401714003884http://hdl.handle.net/20.500.12123/43720304-4017https://doi.org/10.1016/j.vetpar.2014.07.003Veterinary Parasitology 205 (1–2) : 46-56 (September 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:49Zoai:localhost:20.500.12123/4372instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:49.457INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
title Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
spellingShingle Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
Katzer, Frank
Cordero
Inmunización
Toxoplasma gondii
Vacuna
Toxoplasmosis
Zoonosis
Lambs
Immunization
Vaccines
Zoonoses
title_short Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
title_full Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
title_fullStr Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
title_full_unstemmed Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
title_sort Immunization of lambs with the S48 strain of Toxoplasma gondii reduces tissue cyst burden following oral challenge with a complete strain of the parasite
dc.creator.none.fl_str_mv Katzer, Frank
Canton, German Jose
Burrels, Alison
Palarea-Albaladejo, Javier
Horton, Ben
Bartley, Paul M.
Pang, Yvonne
Chianini, Francesca
Innes, Elisabeth A.
Benavides-Silván, Julio
author Katzer, Frank
author_facet Katzer, Frank
Canton, German Jose
Burrels, Alison
Palarea-Albaladejo, Javier
Horton, Ben
Bartley, Paul M.
Pang, Yvonne
Chianini, Francesca
Innes, Elisabeth A.
Benavides-Silván, Julio
author_role author
author2 Canton, German Jose
Burrels, Alison
Palarea-Albaladejo, Javier
Horton, Ben
Bartley, Paul M.
Pang, Yvonne
Chianini, Francesca
Innes, Elisabeth A.
Benavides-Silván, Julio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cordero
Inmunización
Toxoplasma gondii
Vacuna
Toxoplasmosis
Zoonosis
Lambs
Immunization
Vaccines
Zoonoses
topic Cordero
Inmunización
Toxoplasma gondii
Vacuna
Toxoplasmosis
Zoonosis
Lambs
Immunization
Vaccines
Zoonoses
dc.description.none.fl_txt_mv This study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at significantly (p < 0.0001) lower levels in samples from the vaccinated/challenged group (0% in heart and 5.9% in skeletal muscles), when compared to the non-vaccinated/challenged animals (75% heart, 87.9% skeletal muscle). S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. Non-vaccinated/challenged animals showed more frequent lesions in muscles and central nervous system than the vaccinated animals. These results demonstrate that vaccination of lambs with the incomplete S48 T. gondii strain, can protect against establishment of tissue cysts following challenge with a complete strain of T. gondii. Consumption of undercooked meat containing T. gondii cysts is a major route of transmission to people, therefore vaccination of food animals may improve the safety of meat for human consumption.
EEA Balcarce
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Burrels, Alison. Moredun Research Institute; Reino Unido
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Horton, Ben. Moredun Research Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
description This study evaluates the influence of immunizing lambs with the incomplete S48 strain of Toxoplasma gondii, on parasite dissemination following a live oral challenge with a complete strain of T. gondii (M4). Lambs were culled at 14, 28 and 42 days post challenge. Parasite DNA was detected at significantly (p < 0.0001) lower levels in samples from the vaccinated/challenged group (0% in heart and 5.9% in skeletal muscles), when compared to the non-vaccinated/challenged animals (75% heart, 87.9% skeletal muscle). S48 T. gondii DNA was found in muscle or lymph nodes until 42 days post infection, suggesting that parasite DNA or tachyzoites could persist longer after immunization than previously thought. Non-vaccinated/challenged animals showed more frequent lesions in muscles and central nervous system than the vaccinated animals. These results demonstrate that vaccination of lambs with the incomplete S48 T. gondii strain, can protect against establishment of tissue cysts following challenge with a complete strain of T. gondii. Consumption of undercooked meat containing T. gondii cysts is a major route of transmission to people, therefore vaccination of food animals may improve the safety of meat for human consumption.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-15
2019-02-01T12:16:51Z
2019-02-01T12:16:51Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0304401714003884
http://hdl.handle.net/20.500.12123/4372
0304-4017
https://doi.org/10.1016/j.vetpar.2014.07.003
url https://www.sciencedirect.com/science/article/pii/S0304401714003884
http://hdl.handle.net/20.500.12123/4372
https://doi.org/10.1016/j.vetpar.2014.07.003
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology 205 (1–2) : 46-56 (September 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846787517998694400
score 12.982451

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