Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples

Autores
Jauregui, Gloria R.; Fontana, Diego; Micheloud, Juan Francisco; Prieto, Claudio; Delgado, Fernando Oscar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
Instituto de Patobiología
Fil: Jauregui, Gloria R. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fontana, Diego. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Universidad del Salvador. Carrera de Veterinaria; Argentina
Fuente
Brazilian journal of veterinary pathology 12 (2) : 27–32.(Julio 2019)
Materia
Rabies Virus
Immunohistochemistry
Diagnostic Techniques
Cattle
Virus de la Rabia
Inmunohistoquímica
Técnicas de Diagnosis
Ganado Bovino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samplesJauregui, Gloria R.Fontana, DiegoMicheloud, Juan FranciscoPrieto, ClaudioDelgado, Fernando OscarRabies VirusImmunohistochemistryDiagnostic TechniquesCattleVirus de la RabiaInmunohistoquímicaTécnicas de DiagnosisGanado BovinoRabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.Instituto de PatobiologíaFil: Jauregui, Gloria R. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fontana, Diego. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo BiotecnológicoFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; ArgentinaFil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo BiotecnológicoFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Universidad del Salvador. Carrera de Veterinaria; ArgentinaBrazilian Association of Veterinary Pathology2019-11-07T17:18:37Z2019-11-07T17:18:37Z2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdfhttp://hdl.handle.net/20.500.12123/63111983-0246Brazilian journal of veterinary pathology 12 (2) : 27–32.(Julio 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:49Zoai:localhost:20.500.12123/6311instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:49.892INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
spellingShingle Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
Jauregui, Gloria R.
Rabies Virus
Immunohistochemistry
Diagnostic Techniques
Cattle
Virus de la Rabia
Inmunohistoquímica
Técnicas de Diagnosis
Ganado Bovino
title_short Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_full Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_fullStr Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_full_unstemmed Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_sort Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
dc.creator.none.fl_str_mv Jauregui, Gloria R.
Fontana, Diego
Micheloud, Juan Francisco
Prieto, Claudio
Delgado, Fernando Oscar
author Jauregui, Gloria R.
author_facet Jauregui, Gloria R.
Fontana, Diego
Micheloud, Juan Francisco
Prieto, Claudio
Delgado, Fernando Oscar
author_role author
author2 Fontana, Diego
Micheloud, Juan Francisco
Prieto, Claudio
Delgado, Fernando Oscar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Rabies Virus
Immunohistochemistry
Diagnostic Techniques
Cattle
Virus de la Rabia
Inmunohistoquímica
Técnicas de Diagnosis
Ganado Bovino
topic Rabies Virus
Immunohistochemistry
Diagnostic Techniques
Cattle
Virus de la Rabia
Inmunohistoquímica
Técnicas de Diagnosis
Ganado Bovino
dc.description.none.fl_txt_mv Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
Instituto de Patobiología
Fil: Jauregui, Gloria R. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fontana, Diego. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta. Grupo de Trabajo de Patología, Epidemiología e Investigación Diagnóstica. Área de Sanidad Animal; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Biotecnológico
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Universidad del Salvador. Carrera de Veterinaria; Argentina
description Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-07T17:18:37Z
2019-11-07T17:18:37Z
2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdf
http://hdl.handle.net/20.500.12123/6311
1983-0246
url https://bjvp.org.br/wp-content/uploads/2019/07/v12-n2-1.pdf
http://hdl.handle.net/20.500.12123/6311
identifier_str_mv 1983-0246
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA/1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Association of Veterinary Pathology
publisher.none.fl_str_mv Brazilian Association of Veterinary Pathology
dc.source.none.fl_str_mv Brazilian journal of veterinary pathology 12 (2) : 27–32.(Julio 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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