Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples

Autores
Jauregui, Gloria Rosa; Fontana, Diego Sebastian; Micheloud, Juan Francisco; Prieto, Claudio; Delgado, Fernando Oscar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
Fil: Jauregui, Gloria Rosa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Fontana, Diego Sebastian. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Desarrollo Biotecnologico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Desarrollo Biotecnologico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Materia
RABIES
CATTLE
DIAGNOSTIC
IMMUNOHISTOCHEMESTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/118695
Acceder
id CONICETDig_35d76bb843c372be413f7e42626846b3
oai_identifier_str oai:ri.conicet.gov.ar:11336/118695
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samplesJauregui, Gloria RosaFontana, Diego SebastianMicheloud, Juan FranciscoPrieto, ClaudioDelgado, Fernando OscarRABIESCATTLEDIAGNOSTICIMMUNOHISTOCHEMESTRYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.Fil: Jauregui, Gloria Rosa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Fontana, Diego Sebastian. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Desarrollo Biotecnologico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Desarrollo Biotecnologico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaBrazilian Association of Veterinary Pathology2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/118695Jauregui, Gloria Rosa; Fontana, Diego Sebastian; Micheloud, Juan Francisco; Prieto, Claudio; Delgado, Fernando Oscar; Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples; Brazilian Association of Veterinary Pathology; Brazilian Journal of Veterinary Pathology; 12; 2; 5-2019; 27-321983-0246CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bjvp.org.br/bjvp-archive/2019/07/vol-12-n-2-july-2019info:eu-repo/semantics/altIdentifier/doi/10.24070/bjvp.1983-0246.v12i2p27-32info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:12Zoai:ri.conicet.gov.ar:11336/118695instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:12.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
spellingShingle Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
Jauregui, Gloria Rosa
RABIES
CATTLE
DIAGNOSTIC
IMMUNOHISTOCHEMESTRY
title_short Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_full Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_fullStr Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_full_unstemmed Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
title_sort Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples
dc.creator.none.fl_str_mv Jauregui, Gloria Rosa
Fontana, Diego Sebastian
Micheloud, Juan Francisco
Prieto, Claudio
Delgado, Fernando Oscar
author Jauregui, Gloria Rosa
author_facet Jauregui, Gloria Rosa
Fontana, Diego Sebastian
Micheloud, Juan Francisco
Prieto, Claudio
Delgado, Fernando Oscar
author_role author
author2 Fontana, Diego Sebastian
Micheloud, Juan Francisco
Prieto, Claudio
Delgado, Fernando Oscar
author2_role author
author
author
author
dc.subject.none.fl_str_mv RABIES
CATTLE
DIAGNOSTIC
IMMUNOHISTOCHEMESTRY
topic RABIES
CATTLE
DIAGNOSTIC
IMMUNOHISTOCHEMESTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
Fil: Jauregui, Gloria Rosa. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Fontana, Diego Sebastian. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Desarrollo Biotecnologico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Prieto, Claudio. Universidad Nacional del Litoral. Facultad de Bioquimica y Ciencias Biologicas. Laboratorio de Desarrollo Biotecnologico.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
description Rabies is an important zoonosis with impact on livestock production. The diagnosis is usually based on the rabies virus detection in fresh or refrigerated brain samples by direct immunofluorescence (IF) performed on fixed brain smears. The aim of this study was to evaluate the performance of 6 monoclonal and 1 rabbit-polyclonal new antibodies for rabies diagnostic by using immunohistochemistry (IHC), which detects the agent on formalin fixed paraffin embedded tissues samples. Tested with 2 positive and 2 negative cows for rabies at dilutions 1/200 and 1/1000, obtained immunostaining was strong for one monoclonal and weak for the polyclonal at both dilutions. For 3 monoclonals, the immunostaining was weaker at 1/1000 and was negative at both dilutions for 2 monoclonals. Unwanted background was absent and negative samples remained clear for all antibodies. When the first monoclonal was applied on sections of brain of 19 cows with Rabies and 41 control cows at 1/1000 dilution, immunohistochemistry recognized all positive samples and was negative for all control cows. The number of cases analyzed did not allow estimating sensitivity and specificity of the tested assay, but the correlation observed between IF and IHC in both positive and negative samples suggested that accuracy of the test might be good. The results indicated that the tested antibody can detect the rabies virus on formalin fixed tissue samples, and that immunohistochemistry can complement other confirmatory tests when those cannot be performed.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/118695
Jauregui, Gloria Rosa; Fontana, Diego Sebastian; Micheloud, Juan Francisco; Prieto, Claudio; Delgado, Fernando Oscar; Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples; Brazilian Association of Veterinary Pathology; Brazilian Journal of Veterinary Pathology; 12; 2; 5-2019; 27-32
1983-0246
CONICET Digital
CONICET
url http://hdl.handle.net/11336/118695
identifier_str_mv Jauregui, Gloria Rosa; Fontana, Diego Sebastian; Micheloud, Juan Francisco; Prieto, Claudio; Delgado, Fernando Oscar; Evaluation of new antibodies for the detection of rabies virus in formalin fixed brain tissue samples; Brazilian Association of Veterinary Pathology; Brazilian Journal of Veterinary Pathology; 12; 2; 5-2019; 27-32
1983-0246
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bjvp.org.br/bjvp-archive/2019/07/vol-12-n-2-july-2019
info:eu-repo/semantics/altIdentifier/doi/10.24070/bjvp.1983-0246.v12i2p27-32
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Brazilian Association of Veterinary Pathology
publisher.none.fl_str_mv Brazilian Association of Veterinary Pathology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614180086218752
score 13.070432

Publicaciones similares