Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance

Autores
Raineri, Jesica; Caraballo, Luciano; Rigalli, Nicolás; Portapila, Margarita; Otegui, María Elena; Lía Chan, Raquel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performance under waterlogging, both in greenhouse and field trials carried out during three growth cycles. Transgenic plants had increased chlorophyll content, wider stems, more nodal roots, greater total aerial biomass, a higher harvest index, and increased plant grain yield. Under severe defoliation caused by a windstorm during flowering, transgenic genotypes were able to set more grains than controls. This response was confirmed in controlled defoliation assays. Hybrids generated by crossing B73 HaHB11 lines with the contrasting Mo17 lines were also tested in the field and exhibited the same beneficial traits as the parental lines, compared with their respective controls. Moreover, they were less penalized by stress than commercial hybrids. Waterlogging tolerance increased via improvement of the root system, including more xylem vessels, reduced tissue damage, less superoxide accumulation, and altered carbohydrate metabolism. Multivariate analyses corroborated the robustness of the differential traits observed. Furthermore, canopy spectral reflectance data, computing 29 vegetation indices associated with biomass, chlorophyll, and abiotic stress, helped to distinguish genotypes as well as their growing conditions. Altogether the results reported here indicate that this sunflower gene constitutes a suitable tool to improve maize plants for environments prone to waterlogging and/or wind defoliation.
EEA Pergamino
Fil: Raineri, Jesica. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Caraballo, Luciano. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Rigalli, Nicolás. Universidad Nacional de Rosario—CONICET. CIFASIS; Argentina
Fil: Portapila, Margarita. Universidad Nacional de Rosario—CONICET. CIFASIS; Argentina
Fil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina
Fil: Otegui, María E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Otegui, María E. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; Argentina
Fil: Lía Chan, Raquel. Universidad Nacional del Litoral—CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fuente
Plant Physiology 189 (1) : 230-247. (May 2022)
Materia
Girasol
Maíz
Rendimiento de Cultivos
Mejoramiento Genético
Genetic Improvement
Defoliación
Genotipos
Sunflowers
Maize
Crop Yield
Defoliation
Genotypes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12217
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12217
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation toleranceRaineri, JesicaCaraballo, LucianoRigalli, NicolásPortapila, MargaritaOtegui, María ElenaLía Chan, RaquelGirasolMaízRendimiento de CultivosMejoramiento GenéticoGenetic ImprovementDefoliaciónGenotiposSunflowersMaizeCrop YieldDefoliationGenotypesThe sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performance under waterlogging, both in greenhouse and field trials carried out during three growth cycles. Transgenic plants had increased chlorophyll content, wider stems, more nodal roots, greater total aerial biomass, a higher harvest index, and increased plant grain yield. Under severe defoliation caused by a windstorm during flowering, transgenic genotypes were able to set more grains than controls. This response was confirmed in controlled defoliation assays. Hybrids generated by crossing B73 HaHB11 lines with the contrasting Mo17 lines were also tested in the field and exhibited the same beneficial traits as the parental lines, compared with their respective controls. Moreover, they were less penalized by stress than commercial hybrids. Waterlogging tolerance increased via improvement of the root system, including more xylem vessels, reduced tissue damage, less superoxide accumulation, and altered carbohydrate metabolism. Multivariate analyses corroborated the robustness of the differential traits observed. Furthermore, canopy spectral reflectance data, computing 29 vegetation indices associated with biomass, chlorophyll, and abiotic stress, helped to distinguish genotypes as well as their growing conditions. Altogether the results reported here indicate that this sunflower gene constitutes a suitable tool to improve maize plants for environments prone to waterlogging and/or wind defoliation.EEA PergaminoFil: Raineri, Jesica. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Caraballo, Luciano. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Rigalli, Nicolás. Universidad Nacional de Rosario—CONICET. CIFASIS; ArgentinaFil: Portapila, Margarita. Universidad Nacional de Rosario—CONICET. CIFASIS; ArgentinaFil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; ArgentinaFil: Otegui, María E. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Otegui, María E. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; ArgentinaFil: Lía Chan, Raquel. Universidad Nacional del Litoral—CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaOxford University Press2022-07-01T15:46:04Z2022-07-01T15:46:04Z2022-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12217https://academic.oup.com/plphys/article-abstract/189/1/230/65272170032-08891532-2548 (online)https://doi.org/10.1093/plphys/kiac054Plant Physiology 189 (1) : 230-247. (May 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-06T09:41:15Zoai:localhost:20.500.12123/12217instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:41:15.82INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
title Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
spellingShingle Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
Raineri, Jesica
Girasol
Maíz
Rendimiento de Cultivos
Mejoramiento Genético
Genetic Improvement
Defoliación
Genotipos
Sunflowers
Maize
Crop Yield
Defoliation
Genotypes
title_short Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
title_full Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
title_fullStr Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
title_full_unstemmed Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
title_sort Expressing the sunflower transcription factor HaHB11 in maize improves waterlogging and defoliation tolerance
dc.creator.none.fl_str_mv Raineri, Jesica
Caraballo, Luciano
Rigalli, Nicolás
Portapila, Margarita
Otegui, María Elena
Lía Chan, Raquel
author Raineri, Jesica
author_facet Raineri, Jesica
Caraballo, Luciano
Rigalli, Nicolás
Portapila, Margarita
Otegui, María Elena
Lía Chan, Raquel
author_role author
author2 Caraballo, Luciano
Rigalli, Nicolás
Portapila, Margarita
Otegui, María Elena
Lía Chan, Raquel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Girasol
Maíz
Rendimiento de Cultivos
Mejoramiento Genético
Genetic Improvement
Defoliación
Genotipos
Sunflowers
Maize
Crop Yield
Defoliation
Genotypes
topic Girasol
Maíz
Rendimiento de Cultivos
Mejoramiento Genético
Genetic Improvement
Defoliación
Genotipos
Sunflowers
Maize
Crop Yield
Defoliation
Genotypes
dc.description.none.fl_txt_mv The sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performance under waterlogging, both in greenhouse and field trials carried out during three growth cycles. Transgenic plants had increased chlorophyll content, wider stems, more nodal roots, greater total aerial biomass, a higher harvest index, and increased plant grain yield. Under severe defoliation caused by a windstorm during flowering, transgenic genotypes were able to set more grains than controls. This response was confirmed in controlled defoliation assays. Hybrids generated by crossing B73 HaHB11 lines with the contrasting Mo17 lines were also tested in the field and exhibited the same beneficial traits as the parental lines, compared with their respective controls. Moreover, they were less penalized by stress than commercial hybrids. Waterlogging tolerance increased via improvement of the root system, including more xylem vessels, reduced tissue damage, less superoxide accumulation, and altered carbohydrate metabolism. Multivariate analyses corroborated the robustness of the differential traits observed. Furthermore, canopy spectral reflectance data, computing 29 vegetation indices associated with biomass, chlorophyll, and abiotic stress, helped to distinguish genotypes as well as their growing conditions. Altogether the results reported here indicate that this sunflower gene constitutes a suitable tool to improve maize plants for environments prone to waterlogging and/or wind defoliation.
EEA Pergamino
Fil: Raineri, Jesica. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Caraballo, Luciano. Universidad Nacional del Litoral-CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Rigalli, Nicolás. Universidad Nacional de Rosario—CONICET. CIFASIS; Argentina
Fil: Portapila, Margarita. Universidad Nacional de Rosario—CONICET. CIFASIS; Argentina
Fil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina
Fil: Otegui, María E. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Otegui, María E. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal; Argentina
Fil: Lía Chan, Raquel. Universidad Nacional del Litoral—CONICET. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
description The sunflower (Helianthus annuus) transcription factor HaHB11 (H. annuus Homeobox 11) belongs to the homeodomain-leucine zipper family and confers improved yield to maize (Zea mays) hybrids (HiII × B73) and lines. Here we report that transgenic maize lines expressing HaHB11 exhibited better performance under waterlogging, both in greenhouse and field trials carried out during three growth cycles. Transgenic plants had increased chlorophyll content, wider stems, more nodal roots, greater total aerial biomass, a higher harvest index, and increased plant grain yield. Under severe defoliation caused by a windstorm during flowering, transgenic genotypes were able to set more grains than controls. This response was confirmed in controlled defoliation assays. Hybrids generated by crossing B73 HaHB11 lines with the contrasting Mo17 lines were also tested in the field and exhibited the same beneficial traits as the parental lines, compared with their respective controls. Moreover, they were less penalized by stress than commercial hybrids. Waterlogging tolerance increased via improvement of the root system, including more xylem vessels, reduced tissue damage, less superoxide accumulation, and altered carbohydrate metabolism. Multivariate analyses corroborated the robustness of the differential traits observed. Furthermore, canopy spectral reflectance data, computing 29 vegetation indices associated with biomass, chlorophyll, and abiotic stress, helped to distinguish genotypes as well as their growing conditions. Altogether the results reported here indicate that this sunflower gene constitutes a suitable tool to improve maize plants for environments prone to waterlogging and/or wind defoliation.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-01T15:46:04Z
2022-07-01T15:46:04Z
2022-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12217
https://academic.oup.com/plphys/article-abstract/189/1/230/6527217
0032-0889
1532-2548 (online)
https://doi.org/10.1093/plphys/kiac054
url http://hdl.handle.net/20.500.12123/12217
https://academic.oup.com/plphys/article-abstract/189/1/230/6527217
https://doi.org/10.1093/plphys/kiac054
identifier_str_mv 0032-0889
1532-2548 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Plant Physiology 189 (1) : 230-247. (May 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.084122

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