Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type

Autores
Gonzalez, Fernanda Gabriela; Capella, Matías; Ribichich, Karina Fabiana; Curín, Facundo; Giacomelli, Jorge Ignacio; Ayala, Francisco; Watson, Gerónimo; Otegui, María Elena; Lía Chan, Raquel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts.
EEA Pergamino
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento Ecofisiología; Argentina.CONICET-UNNOBA.CITNOBA; Argentina
Fil: Capella, Matías. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ribichich, Karina Fabiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Curín, Facundo. CONICET-UNNOBA. CITNOBA, (Pergamino); Argentina
Fil: Giacomelli, Jorge Ignacio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ayala, Francisco. INDER/BIOCRES (Rosario); Argentina
Fil: Watson, Gerónimo. INDEAR/BIOCERES (Rosario); Argentina
Fil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Lía Chan, Raquel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fuente
Journal of Experimental Botany 70 (5) : 1669–1681 (February 2019)
Materia
Helianthus annuus
Genes
Rendimiento
Semilla
Uso del Agua
Transgénicos
Ensayo de Semillas
Sunflower
Yields
Seeds
Water Use
Transgenics
Seed Testing
Girasol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4784
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4784
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild typeGonzalez, Fernanda GabrielaCapella, MatíasRibichich, Karina FabianaCurín, FacundoGiacomelli, Jorge IgnacioAyala, FranciscoWatson, GerónimoOtegui, María ElenaLía Chan, RaquelHelianthus annuusGenesRendimientoSemillaUso del AguaTransgénicosEnsayo de SemillasSunflowerYieldsSeedsWater UseTransgenicsSeed TestingGirasolHaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts.EEA PergaminoFil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento Ecofisiología; Argentina.CONICET-UNNOBA.CITNOBA; ArgentinaFil: Capella, Matías. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Ribichich, Karina Fabiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Curín, Facundo. CONICET-UNNOBA. CITNOBA, (Pergamino); ArgentinaFil: Giacomelli, Jorge Ignacio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Ayala, Francisco. INDER/BIOCRES (Rosario); ArgentinaFil: Watson, Gerónimo. INDEAR/BIOCERES (Rosario); ArgentinaFil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Lía Chan, Raquel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina2019-03-29T17:16:19Z2019-03-29T17:16:19Z2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://academic.oup.com/jxb/article/70/5/1669/5307521http://hdl.handle.net/20.500.12123/47840022-09571460-2431 (digital)https://doi.org/10.1093/jxb/erz037Journal of Experimental Botany 70 (5) : 1669–1681 (February 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:29Zoai:localhost:20.500.12123/4784instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:29.658INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
spellingShingle Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
Gonzalez, Fernanda Gabriela
Helianthus annuus
Genes
Rendimiento
Semilla
Uso del Agua
Transgénicos
Ensayo de Semillas
Sunflower
Yields
Seeds
Water Use
Transgenics
Seed Testing
Girasol
title_short Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_full Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_fullStr Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_full_unstemmed Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
title_sort Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type
dc.creator.none.fl_str_mv Gonzalez, Fernanda Gabriela
Capella, Matías
Ribichich, Karina Fabiana
Curín, Facundo
Giacomelli, Jorge Ignacio
Ayala, Francisco
Watson, Gerónimo
Otegui, María Elena
Lía Chan, Raquel
author Gonzalez, Fernanda Gabriela
author_facet Gonzalez, Fernanda Gabriela
Capella, Matías
Ribichich, Karina Fabiana
Curín, Facundo
Giacomelli, Jorge Ignacio
Ayala, Francisco
Watson, Gerónimo
Otegui, María Elena
Lía Chan, Raquel
author_role author
author2 Capella, Matías
Ribichich, Karina Fabiana
Curín, Facundo
Giacomelli, Jorge Ignacio
Ayala, Francisco
Watson, Gerónimo
Otegui, María Elena
Lía Chan, Raquel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Helianthus annuus
Genes
Rendimiento
Semilla
Uso del Agua
Transgénicos
Ensayo de Semillas
Sunflower
Yields
Seeds
Water Use
Transgenics
Seed Testing
Girasol
topic Helianthus annuus
Genes
Rendimiento
Semilla
Uso del Agua
Transgénicos
Ensayo de Semillas
Sunflower
Yields
Seeds
Water Use
Transgenics
Seed Testing
Girasol
dc.description.none.fl_txt_mv HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts.
EEA Pergamino
Fil: González, Fernanda Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento Ecofisiología; Argentina.CONICET-UNNOBA.CITNOBA; Argentina
Fil: Capella, Matías. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ribichich, Karina Fabiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Curín, Facundo. CONICET-UNNOBA. CITNOBA, (Pergamino); Argentina
Fil: Giacomelli, Jorge Ignacio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Ayala, Francisco. INDER/BIOCRES (Rosario); Argentina
Fil: Watson, Gerónimo. INDEAR/BIOCERES (Rosario); Argentina
Fil: Otegui, María Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Lía Chan, Raquel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Instituto de Agrobiotecnología del Litoral; Argentina
description HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4’s action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-29T17:16:19Z
2019-03-29T17:16:19Z
2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://academic.oup.com/jxb/article/70/5/1669/5307521
http://hdl.handle.net/20.500.12123/4784
0022-0957
1460-2431 (digital)
https://doi.org/10.1093/jxb/erz037
url https://academic.oup.com/jxb/article/70/5/1669/5307521
http://hdl.handle.net/20.500.12123/4784
https://doi.org/10.1093/jxb/erz037
identifier_str_mv 0022-0957
1460-2431 (digital)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Experimental Botany 70 (5) : 1669–1681 (February 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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