Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments

Autores
Dominguez, Johanna Elizabeth; Lovera, Rosario; Sánchez, Juliana; Redondo, Leandro Martin; Chacana, Pablo; Cavia, Regino; Fernandez Miyakawa, Mariano Enrique
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long-term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR). Farm environments serve as AMR sources, resulting in the transmission of antimicrobial-resistant bacteria and their AMR genes of livestock origin into wildlife. This study aimed to analyse the profile of enterobacteria carrying AMR determinants in rats captured in livestock farms to determine their potential vectors as for the spread of AMR. To this end, 56 rats (52 R. norvegicus and 4 R. rattus) were live-trapped on 11 farms (pig, dairy, poultry and mixed farms) located in central Argentina, from spring 2016 to autumn 2017. From 50 of the R. norvegicus individuals and three of the R. rattus individuals found in 10 of the farms, we isolated 53 Escherichia coli and five Salmonella strains. Susceptibility to antimicrobials, genotypic profiles, minimal inhibitory concentration of colistin and the presence of mcr-1 and genes encoding extended-spectrum β-lactamase (ESBL) were determined. Of the 58 isolates not susceptible to different antimicrobial classes, 28 of the E. coli strains and two of the Salmonella strains were defined as multi-drug resistant (MDR). S. Westhampton and S. Newport recovered were not susceptible to ampicillin or all the cephems tested. One of the E. coli obtained showed resistance to colistin and harboured the mcr-1 gene, demonstrated by PCR and conjugation. In two ESBL-producing Salmonella isolated from rats, CTX-M-2 genes were responsible for the observed resistance to third-generation cephalosporins. The MDR E. coli isolates showed several different resistance patterns (23), although some of them were the same in different individuals and different farms, with six resistance patterns, evidencing the dispersion of strains. These findings suggest that rats play a role in the dissemination of AMR determinants between animal, humans and environmental reservoirs.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Sánchez, Juliana. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Centro de Bioinvestigaciones-CeBio. Laboratorio de Investigación y Desarrollo en Agrobiología; Argentina
Fil: Redondo, Leandro Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Redondo, Leandro Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Zoonoses and Public Health : 1-9 (First published: 04 July 2023)
Materia
Enterobacteriaceae
Livestock
Drug Resistance
Rats
Antimicrobial Resistance
Vectors
Ganado
Resistencia a Medicamentos
Rata
Resistencia a los Antimicrobianos
Vectores
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14786
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14786
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environmentsDominguez, Johanna ElizabethLovera, RosarioSánchez, JulianaRedondo, Leandro MartinChacana, PabloCavia, ReginoFernandez Miyakawa, Mariano EnriqueEnterobacteriaceaeLivestockDrug ResistanceRatsAntimicrobial ResistanceVectorsGanadoResistencia a MedicamentosRataResistencia a los AntimicrobianosVectoresRattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long-term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR). Farm environments serve as AMR sources, resulting in the transmission of antimicrobial-resistant bacteria and their AMR genes of livestock origin into wildlife. This study aimed to analyse the profile of enterobacteria carrying AMR determinants in rats captured in livestock farms to determine their potential vectors as for the spread of AMR. To this end, 56 rats (52 R. norvegicus and 4 R. rattus) were live-trapped on 11 farms (pig, dairy, poultry and mixed farms) located in central Argentina, from spring 2016 to autumn 2017. From 50 of the R. norvegicus individuals and three of the R. rattus individuals found in 10 of the farms, we isolated 53 Escherichia coli and five Salmonella strains. Susceptibility to antimicrobials, genotypic profiles, minimal inhibitory concentration of colistin and the presence of mcr-1 and genes encoding extended-spectrum β-lactamase (ESBL) were determined. Of the 58 isolates not susceptible to different antimicrobial classes, 28 of the E. coli strains and two of the Salmonella strains were defined as multi-drug resistant (MDR). S. Westhampton and S. Newport recovered were not susceptible to ampicillin or all the cephems tested. One of the E. coli obtained showed resistance to colistin and harboured the mcr-1 gene, demonstrated by PCR and conjugation. In two ESBL-producing Salmonella isolated from rats, CTX-M-2 genes were responsible for the observed resistance to third-generation cephalosporins. The MDR E. coli isolates showed several different resistance patterns (23), although some of them were the same in different individuals and different farms, with six resistance patterns, evidencing the dispersion of strains. These findings suggest that rats play a role in the dissemination of AMR determinants between animal, humans and environmental reservoirs.Instituto de PatobiologíaFil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Sánchez, Juliana. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Centro de Bioinvestigaciones-CeBio. Laboratorio de Investigación y Desarrollo en Agrobiología; ArgentinaFil: Redondo, Leandro Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Redondo, Leandro Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2023-07-20T13:31:27Z2023-07-20T13:31:27Z2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14786https://onlinelibrary.wiley.com/doi/10.1111/zph.130711863-2378https://doi.org/10.1111/zph.13071Zoonoses and Public Health : 1-9 (First published: 04 July 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las aves.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:00Zoai:localhost:20.500.12123/14786instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:01.105INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
title Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
spellingShingle Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
Dominguez, Johanna Elizabeth
Enterobacteriaceae
Livestock
Drug Resistance
Rats
Antimicrobial Resistance
Vectors
Ganado
Resistencia a Medicamentos
Rata
Resistencia a los Antimicrobianos
Vectores
title_short Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
title_full Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
title_fullStr Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
title_full_unstemmed Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
title_sort Rats as sources of multidrug-resistant Enterobacteriaceae in animal production environments
dc.creator.none.fl_str_mv Dominguez, Johanna Elizabeth
Lovera, Rosario
Sánchez, Juliana
Redondo, Leandro Martin
Chacana, Pablo
Cavia, Regino
Fernandez Miyakawa, Mariano Enrique
author Dominguez, Johanna Elizabeth
author_facet Dominguez, Johanna Elizabeth
Lovera, Rosario
Sánchez, Juliana
Redondo, Leandro Martin
Chacana, Pablo
Cavia, Regino
Fernandez Miyakawa, Mariano Enrique
author_role author
author2 Lovera, Rosario
Sánchez, Juliana
Redondo, Leandro Martin
Chacana, Pablo
Cavia, Regino
Fernandez Miyakawa, Mariano Enrique
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Enterobacteriaceae
Livestock
Drug Resistance
Rats
Antimicrobial Resistance
Vectors
Ganado
Resistencia a Medicamentos
Rata
Resistencia a los Antimicrobianos
Vectores
topic Enterobacteriaceae
Livestock
Drug Resistance
Rats
Antimicrobial Resistance
Vectors
Ganado
Resistencia a Medicamentos
Rata
Resistencia a los Antimicrobianos
Vectores
dc.description.none.fl_txt_mv Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long-term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR). Farm environments serve as AMR sources, resulting in the transmission of antimicrobial-resistant bacteria and their AMR genes of livestock origin into wildlife. This study aimed to analyse the profile of enterobacteria carrying AMR determinants in rats captured in livestock farms to determine their potential vectors as for the spread of AMR. To this end, 56 rats (52 R. norvegicus and 4 R. rattus) were live-trapped on 11 farms (pig, dairy, poultry and mixed farms) located in central Argentina, from spring 2016 to autumn 2017. From 50 of the R. norvegicus individuals and three of the R. rattus individuals found in 10 of the farms, we isolated 53 Escherichia coli and five Salmonella strains. Susceptibility to antimicrobials, genotypic profiles, minimal inhibitory concentration of colistin and the presence of mcr-1 and genes encoding extended-spectrum β-lactamase (ESBL) were determined. Of the 58 isolates not susceptible to different antimicrobial classes, 28 of the E. coli strains and two of the Salmonella strains were defined as multi-drug resistant (MDR). S. Westhampton and S. Newport recovered were not susceptible to ampicillin or all the cephems tested. One of the E. coli obtained showed resistance to colistin and harboured the mcr-1 gene, demonstrated by PCR and conjugation. In two ESBL-producing Salmonella isolated from rats, CTX-M-2 genes were responsible for the observed resistance to third-generation cephalosporins. The MDR E. coli isolates showed several different resistance patterns (23), although some of them were the same in different individuals and different farms, with six resistance patterns, evidencing the dispersion of strains. These findings suggest that rats play a role in the dissemination of AMR determinants between animal, humans and environmental reservoirs.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lovera, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Lovera, Rosario. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Sánchez, Juliana. Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires. Centro de Bioinvestigaciones-CeBio. Laboratorio de Investigación y Desarrollo en Agrobiología; Argentina
Fil: Redondo, Leandro Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Redondo, Leandro Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Cavia, Regino. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Fernandez Miyakawa, Mariano Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Rattus norvegicus and Rattus rattus are commensal pest rodents, considered reservoirs and vectors of zoonotic pathogens. In livestock farms, the wide use of antimicrobials and their release into the environment lead to high long-term residual concentrations, which may in turn lead to the occurrence of antimicrobial resistance (AMR). Farm environments serve as AMR sources, resulting in the transmission of antimicrobial-resistant bacteria and their AMR genes of livestock origin into wildlife. This study aimed to analyse the profile of enterobacteria carrying AMR determinants in rats captured in livestock farms to determine their potential vectors as for the spread of AMR. To this end, 56 rats (52 R. norvegicus and 4 R. rattus) were live-trapped on 11 farms (pig, dairy, poultry and mixed farms) located in central Argentina, from spring 2016 to autumn 2017. From 50 of the R. norvegicus individuals and three of the R. rattus individuals found in 10 of the farms, we isolated 53 Escherichia coli and five Salmonella strains. Susceptibility to antimicrobials, genotypic profiles, minimal inhibitory concentration of colistin and the presence of mcr-1 and genes encoding extended-spectrum β-lactamase (ESBL) were determined. Of the 58 isolates not susceptible to different antimicrobial classes, 28 of the E. coli strains and two of the Salmonella strains were defined as multi-drug resistant (MDR). S. Westhampton and S. Newport recovered were not susceptible to ampicillin or all the cephems tested. One of the E. coli obtained showed resistance to colistin and harboured the mcr-1 gene, demonstrated by PCR and conjugation. In two ESBL-producing Salmonella isolated from rats, CTX-M-2 genes were responsible for the observed resistance to third-generation cephalosporins. The MDR E. coli isolates showed several different resistance patterns (23), although some of them were the same in different individuals and different farms, with six resistance patterns, evidencing the dispersion of strains. These findings suggest that rats play a role in the dissemination of AMR determinants between animal, humans and environmental reservoirs.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-20T13:31:27Z
2023-07-20T13:31:27Z
2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14786
https://onlinelibrary.wiley.com/doi/10.1111/zph.13071
1863-2378
https://doi.org/10.1111/zph.13071
url http://hdl.handle.net/20.500.12123/14786
https://onlinelibrary.wiley.com/doi/10.1111/zph.13071
https://doi.org/10.1111/zph.13071
identifier_str_mv 1863-2378
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA-1115056/AR./Enfermedades infecciosas de las aves.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Zoonoses and Public Health : 1-9 (First published: 04 July 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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