Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus

Autores
Morrell, Eleonora Lidia; Barbeito, Claudio Gustavo; Odeon, Anselmo Carlos; Gimeno, Eduardo Juan; Campero, Carlos Manuel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC.
EEA Balcarce
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fuente
Reproduction in Domestic Animals 46 (2) : 309-315 (April 2011)
Materia
Ganado Bovino
Enfermedades de los Animales
Campylobacter fetus
Aborto
Histopatología
Inmunohistoquímica
PCR
Diagnóstico
Cattle
Animal Diseases
Abortion
Histopathology
Immunohistochemistry
Diagnosis
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6030
Acceder
id INTADig_7aea2e0df2d1d6129bfc49dc6898238b
oai_identifier_str oai:localhost:20.500.12123/6030
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetusMorrell, Eleonora LidiaBarbeito, Claudio GustavoOdeon, Anselmo CarlosGimeno, Eduardo JuanCampero, Carlos ManuelGanado BovinoEnfermedades de los AnimalesCampylobacter fetusAbortoHistopatologíaInmunohistoquímicaPCRDiagnósticoCattleAnimal DiseasesAbortionHistopathologyImmunohistochemistryDiagnosisReacción en Cadena de la PolimerasaPolymerase Chain ReactionBovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC.EEA BalcarceFil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; ArgentinaFil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaWiley2019-10-01T14:33:38Z2019-10-01T14:33:38Z2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.xhttp://hdl.handle.net/20.500.12123/60300936-67681439-0531https://doi.org/10.1111/j.1439-0531.2010.01668.xReproduction in Domestic Animals 46 (2) : 309-315 (April 2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:39Zoai:localhost:20.500.12123/6030instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:39.389INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
spellingShingle Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
Morrell, Eleonora Lidia
Ganado Bovino
Enfermedades de los Animales
Campylobacter fetus
Aborto
Histopatología
Inmunohistoquímica
PCR
Diagnóstico
Cattle
Animal Diseases
Abortion
Histopathology
Immunohistochemistry
Diagnosis
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
title_short Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_full Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_fullStr Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_full_unstemmed Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_sort Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
dc.creator.none.fl_str_mv Morrell, Eleonora Lidia
Barbeito, Claudio Gustavo
Odeon, Anselmo Carlos
Gimeno, Eduardo Juan
Campero, Carlos Manuel
author Morrell, Eleonora Lidia
author_facet Morrell, Eleonora Lidia
Barbeito, Claudio Gustavo
Odeon, Anselmo Carlos
Gimeno, Eduardo Juan
Campero, Carlos Manuel
author_role author
author2 Barbeito, Claudio Gustavo
Odeon, Anselmo Carlos
Gimeno, Eduardo Juan
Campero, Carlos Manuel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Campylobacter fetus
Aborto
Histopatología
Inmunohistoquímica
PCR
Diagnóstico
Cattle
Animal Diseases
Abortion
Histopathology
Immunohistochemistry
Diagnosis
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
topic Ganado Bovino
Enfermedades de los Animales
Campylobacter fetus
Aborto
Histopatología
Inmunohistoquímica
PCR
Diagnóstico
Cattle
Animal Diseases
Abortion
Histopathology
Immunohistochemistry
Diagnosis
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
dc.description.none.fl_txt_mv Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC.
EEA Balcarce
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Histología y Embriología e Instituto de Patología; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
description Bovine campylobacteriosis (BC) is a venereal disease caused by Campylobacter fetus characterized by temporary infertility with mild endometritis, early embryonic death and occasional abortions. The objectives of this study were to describe and identify C. fetus in spontaneous bovine abortion on the basis of histopathological, immunohistochemical, lectinhistochemical and polymerase chain reaction (PCR) techniques. The most frequent foetal lesion was neutrophilic bronchopneumonia and interstitial pneumonia. Other commonly observed lesions included non‐suppurative interstitial enteritis, hepatitis, pericarditis, myositis, myocarditis, and meningitis. In this study, C. fetus fetus was phenotypically classified in all bovine foetuses from lungs and abomasal fluids. Immunohistochemistry (IHC) staining revealed positive stained Campylobacter organisms with typical morphology. Lectin binding patterns not showed great differences between the infected and the non‐infected groups. The most important changes were a minor peanut agglutinin (PNA) and DBA binding in the alveolar cells of the lungs and DBA globet cells in some of the C. fetus–positive foetuses. Individual variations in each lectin binding pattern complicate the evaluation of the lectins results. All foetuses positive to IHC were positive by PCR. Better efficiency of PCR was obtained from abomasal fluids than from lung tissues. The association of culture and phenotypic techniques with histopathology, IHC and PCR allowed a better characterization and description of BC.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
2019-10-01T14:33:38Z
2019-10-01T14:33:38Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x
http://hdl.handle.net/20.500.12123/6030
0936-6768
1439-0531
https://doi.org/10.1111/j.1439-0531.2010.01668.x
url https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x
http://hdl.handle.net/20.500.12123/6030
https://doi.org/10.1111/j.1439-0531.2010.01668.x
identifier_str_mv 0936-6768
1439-0531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Reproduction in Domestic Animals 46 (2) : 309-315 (April 2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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