Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus

Autores
Morrell, E. L.; Barbeito, Claudio Gustavo; Odeón, C. A.; Gimeno, Eduardo Juan; Campero, C. M.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine campylobacteriosis (BC) is a venereal disease caused byCampylobacter fetus characterized by temporary infertilitywith mild endometritis, early embryonic death and occasionalabortions. The objectives of this study were to describe andidentify C. fetus in spontaneous bovine abortion on the basisof histopathological, immunohistochemical, lectinhistochemicaland polymerase chain reaction (PCR) techniques. The mostfrequent foetal lesion was neutrophilic bronchopneumonia andinterstitial pneumonia. Other commonly observed lesionsincluded non-suppurative interstitial enteritis, hepatitis, pericarditis,myositis, myocarditis, and meningitis. In this study,C. fetus fetus was phenotypically classified in all bovinefoetuses from lungs and abomasal fluids. Immunohistochemistry(IHC) staining revealed positive stained Campylobacterorganisms with typical morphology. Lectin binding patternsnot showed great differences between the infected and the noninfectedgroups. The most important changes were a minorpeanut agglutinin (PNA) and DBA binding in the alveolarcells of the lungs and DBA globet cells in some of the C. fetus?positive foetuses. Individual variations in each lectin bindingpattern complicate the evaluation of the lectins results. Allfoetuses positive to IHC were positive by PCR. Betterefficiency of PCR was obtained from abomasal fluids thanfrom lung tissues. The association of culture and phenotypictechniques with histopathology, IHC and PCR allowed abetter characterization and description of BC.
Fil: Morrell, E. L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Odeón, C. A.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Campero, C. M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Materia
Venereal disease
Carbohydrates
fetal pathology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196184
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/196184
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetusMorrell, E. L.Barbeito, Claudio GustavoOdeón, C. A.Gimeno, Eduardo JuanCampero, C. M.Venereal diseaseCarbohydratesfetal pathologyhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bovine campylobacteriosis (BC) is a venereal disease caused byCampylobacter fetus characterized by temporary infertilitywith mild endometritis, early embryonic death and occasionalabortions. The objectives of this study were to describe andidentify C. fetus in spontaneous bovine abortion on the basisof histopathological, immunohistochemical, lectinhistochemicaland polymerase chain reaction (PCR) techniques. The mostfrequent foetal lesion was neutrophilic bronchopneumonia andinterstitial pneumonia. Other commonly observed lesionsincluded non-suppurative interstitial enteritis, hepatitis, pericarditis,myositis, myocarditis, and meningitis. In this study,C. fetus fetus was phenotypically classified in all bovinefoetuses from lungs and abomasal fluids. Immunohistochemistry(IHC) staining revealed positive stained Campylobacterorganisms with typical morphology. Lectin binding patternsnot showed great differences between the infected and the noninfectedgroups. The most important changes were a minorpeanut agglutinin (PNA) and DBA binding in the alveolarcells of the lungs and DBA globet cells in some of the C. fetus?positive foetuses. Individual variations in each lectin bindingpattern complicate the evaluation of the lectins results. Allfoetuses positive to IHC were positive by PCR. Betterefficiency of PCR was obtained from abomasal fluids thanfrom lung tissues. The association of culture and phenotypictechniques with histopathology, IHC and PCR allowed abetter characterization and description of BC.Fil: Morrell, E. L.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Odeón, C. A.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata; ArgentinaFil: Campero, C. M.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaWiley Blackwell Publishing, Inc2011-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196184Morrell, E. L.; Barbeito, Claudio Gustavo; Odeón, C. A.; Gimeno, Eduardo Juan; Campero, C. M.; Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 2; 1-2011; 309-3150936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2010.01668.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:23Zoai:ri.conicet.gov.ar:11336/196184instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:23.981CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
spellingShingle Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
Morrell, E. L.
Venereal disease
Carbohydrates
fetal pathology
title_short Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_full Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_fullStr Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_full_unstemmed Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
title_sort Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus
dc.creator.none.fl_str_mv Morrell, E. L.
Barbeito, Claudio Gustavo
Odeón, C. A.
Gimeno, Eduardo Juan
Campero, C. M.
author Morrell, E. L.
author_facet Morrell, E. L.
Barbeito, Claudio Gustavo
Odeón, C. A.
Gimeno, Eduardo Juan
Campero, C. M.
author_role author
author2 Barbeito, Claudio Gustavo
Odeón, C. A.
Gimeno, Eduardo Juan
Campero, C. M.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Venereal disease
Carbohydrates
fetal pathology
topic Venereal disease
Carbohydrates
fetal pathology
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bovine campylobacteriosis (BC) is a venereal disease caused byCampylobacter fetus characterized by temporary infertilitywith mild endometritis, early embryonic death and occasionalabortions. The objectives of this study were to describe andidentify C. fetus in spontaneous bovine abortion on the basisof histopathological, immunohistochemical, lectinhistochemicaland polymerase chain reaction (PCR) techniques. The mostfrequent foetal lesion was neutrophilic bronchopneumonia andinterstitial pneumonia. Other commonly observed lesionsincluded non-suppurative interstitial enteritis, hepatitis, pericarditis,myositis, myocarditis, and meningitis. In this study,C. fetus fetus was phenotypically classified in all bovinefoetuses from lungs and abomasal fluids. Immunohistochemistry(IHC) staining revealed positive stained Campylobacterorganisms with typical morphology. Lectin binding patternsnot showed great differences between the infected and the noninfectedgroups. The most important changes were a minorpeanut agglutinin (PNA) and DBA binding in the alveolarcells of the lungs and DBA globet cells in some of the C. fetus?positive foetuses. Individual variations in each lectin bindingpattern complicate the evaluation of the lectins results. Allfoetuses positive to IHC were positive by PCR. Betterefficiency of PCR was obtained from abomasal fluids thanfrom lung tissues. The association of culture and phenotypictechniques with histopathology, IHC and PCR allowed abetter characterization and description of BC.
Fil: Morrell, E. L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Odeón, C. A.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata; Argentina
Fil: Campero, C. M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
description Bovine campylobacteriosis (BC) is a venereal disease caused byCampylobacter fetus characterized by temporary infertilitywith mild endometritis, early embryonic death and occasionalabortions. The objectives of this study were to describe andidentify C. fetus in spontaneous bovine abortion on the basisof histopathological, immunohistochemical, lectinhistochemicaland polymerase chain reaction (PCR) techniques. The mostfrequent foetal lesion was neutrophilic bronchopneumonia andinterstitial pneumonia. Other commonly observed lesionsincluded non-suppurative interstitial enteritis, hepatitis, pericarditis,myositis, myocarditis, and meningitis. In this study,C. fetus fetus was phenotypically classified in all bovinefoetuses from lungs and abomasal fluids. Immunohistochemistry(IHC) staining revealed positive stained Campylobacterorganisms with typical morphology. Lectin binding patternsnot showed great differences between the infected and the noninfectedgroups. The most important changes were a minorpeanut agglutinin (PNA) and DBA binding in the alveolarcells of the lungs and DBA globet cells in some of the C. fetus?positive foetuses. Individual variations in each lectin bindingpattern complicate the evaluation of the lectins results. Allfoetuses positive to IHC were positive by PCR. Betterefficiency of PCR was obtained from abomasal fluids thanfrom lung tissues. The association of culture and phenotypictechniques with histopathology, IHC and PCR allowed abetter characterization and description of BC.
publishDate 2011
dc.date.none.fl_str_mv 2011-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196184
Morrell, E. L.; Barbeito, Claudio Gustavo; Odeón, C. A.; Gimeno, Eduardo Juan; Campero, C. M.; Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 2; 1-2011; 309-315
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196184
identifier_str_mv Morrell, E. L.; Barbeito, Claudio Gustavo; Odeón, C. A.; Gimeno, Eduardo Juan; Campero, C. M.; Histopathological, Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus; Wiley Blackwell Publishing, Inc; Reproduction in Domestic Animals; 46; 2; 1-2011; 309-315
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0531.2010.01668.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0531.2010.01668.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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