Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding

Autores
Armando, Lorena Vanesa; Almada, Pamela Estefania; Tomas, Maria Andrea
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Success in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to increase production in agricultural degraded areas. Its two varieties have high genetic polymorphism and morphological overlapping. The goal of the present study was to assess the genetic diversity and intraspecific structure of a collection of P. coloratum using ISSR molecular markers. Eight accessions of var. makarikariense Gooss. and ten from var. coloratum, plus two commercial materials were analyzed. Differences in variability between varieties were evident: polymorphism and percent of variety specific loci were lower (79%P and 10.3%) in var. makarikariense than in var. coloratum (93%P and 30.9%). Analysis of molecular variation (AMOVA) revealed 13.7% (p = 0.001) of variation was attributable to differences between varieties, and 43.8% remained within the accessions. Individuals of var. coloratum showed higher mean genetic distance among them than the ones of var. makarikariense. The PCO biplot and the dendrogram showed some distinction between varieties, although some accessions of var. coloratum were close to var. makarikariense. Variation in var. coloratum agreed with an allogamous reproductive behavior. Alternatively, less variable closely grouped individuals in var. makarikariense, concur with frequent vegetative reproduction by rhizomes of a reduced number of genotypes. Our study showed that genetic variation had different structure between varieties. This difference should be taken into account when planning germplasm management and conservation and in designing breeding programs.
EEA Rafaela
Fil: Armando, Lorena Vanesa. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Almada, Pamela Estefania. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Tomas, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tomas, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Genetic Resources and Crop Evolution : 1-14. (Published: 02 February 2023)
Materia
Genetic Markers
Intraspecific Relationships
Germplasm
Marcadores Genéticos
Panicum coloratum
Relaciones Intraespecíficas
Germoplasma
Genetic Diversity
Molecular Markers
Perennial Grass
Diversidad Genética
Marcadores Moleculares
Pastura Perenne
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breedingArmando, Lorena VanesaAlmada, Pamela EstefaniaTomas, Maria AndreaGenetic MarkersIntraspecific RelationshipsGermplasmMarcadores GenéticosPanicum coloratumRelaciones IntraespecíficasGermoplasmaGenetic DiversityMolecular MarkersPerennial GrassDiversidad GenéticaMarcadores MolecularesPastura PerenneSuccess in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to increase production in agricultural degraded areas. Its two varieties have high genetic polymorphism and morphological overlapping. The goal of the present study was to assess the genetic diversity and intraspecific structure of a collection of P. coloratum using ISSR molecular markers. Eight accessions of var. makarikariense Gooss. and ten from var. coloratum, plus two commercial materials were analyzed. Differences in variability between varieties were evident: polymorphism and percent of variety specific loci were lower (79%P and 10.3%) in var. makarikariense than in var. coloratum (93%P and 30.9%). Analysis of molecular variation (AMOVA) revealed 13.7% (p = 0.001) of variation was attributable to differences between varieties, and 43.8% remained within the accessions. Individuals of var. coloratum showed higher mean genetic distance among them than the ones of var. makarikariense. The PCO biplot and the dendrogram showed some distinction between varieties, although some accessions of var. coloratum were close to var. makarikariense. Variation in var. coloratum agreed with an allogamous reproductive behavior. Alternatively, less variable closely grouped individuals in var. makarikariense, concur with frequent vegetative reproduction by rhizomes of a reduced number of genotypes. Our study showed that genetic variation had different structure between varieties. This difference should be taken into account when planning germplasm management and conservation and in designing breeding programs.EEA RafaelaFil: Armando, Lorena Vanesa. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Almada, Pamela Estefania. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Tomas, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Tomas, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaSpringer2023-04-26T18:18:01Z2023-04-26T18:18:01Z2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14588https://link.springer.com/article/10.1007/s10722-022-01530-3Armando, L.V., Almada, P.E. & Tomás, M.A. Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding. Genet Resour Crop Evol (2023). https://doi.org/10.1007/s10722-022-01530-31573-5109 (electronic)0925-9864 (print)https://doi.org/10.1007/s10722-022-01530-3Genetic Resources and Crop Evolution : 1-14. (Published: 02 February 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I142-001/2019-PE-E6-I142-001/AR./Mejoramiento genético de leguminosas y gramíneas forrajeras para incrementar la productividad y la sustentabilidad de los sistemas agropecuarios de la Argentinainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:21Zoai:localhost:20.500.12123/14588instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:21.924INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
title Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
spellingShingle Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
Armando, Lorena Vanesa
Genetic Markers
Intraspecific Relationships
Germplasm
Marcadores Genéticos
Panicum coloratum
Relaciones Intraespecíficas
Germoplasma
Genetic Diversity
Molecular Markers
Perennial Grass
Diversidad Genética
Marcadores Moleculares
Pastura Perenne
title_short Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
title_full Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
title_fullStr Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
title_full_unstemmed Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
title_sort Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding
dc.creator.none.fl_str_mv Armando, Lorena Vanesa
Almada, Pamela Estefania
Tomas, Maria Andrea
author Armando, Lorena Vanesa
author_facet Armando, Lorena Vanesa
Almada, Pamela Estefania
Tomas, Maria Andrea
author_role author
author2 Almada, Pamela Estefania
Tomas, Maria Andrea
author2_role author
author
dc.subject.none.fl_str_mv Genetic Markers
Intraspecific Relationships
Germplasm
Marcadores Genéticos
Panicum coloratum
Relaciones Intraespecíficas
Germoplasma
Genetic Diversity
Molecular Markers
Perennial Grass
Diversidad Genética
Marcadores Moleculares
Pastura Perenne
topic Genetic Markers
Intraspecific Relationships
Germplasm
Marcadores Genéticos
Panicum coloratum
Relaciones Intraespecíficas
Germoplasma
Genetic Diversity
Molecular Markers
Perennial Grass
Diversidad Genética
Marcadores Moleculares
Pastura Perenne
dc.description.none.fl_txt_mv Success in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to increase production in agricultural degraded areas. Its two varieties have high genetic polymorphism and morphological overlapping. The goal of the present study was to assess the genetic diversity and intraspecific structure of a collection of P. coloratum using ISSR molecular markers. Eight accessions of var. makarikariense Gooss. and ten from var. coloratum, plus two commercial materials were analyzed. Differences in variability between varieties were evident: polymorphism and percent of variety specific loci were lower (79%P and 10.3%) in var. makarikariense than in var. coloratum (93%P and 30.9%). Analysis of molecular variation (AMOVA) revealed 13.7% (p = 0.001) of variation was attributable to differences between varieties, and 43.8% remained within the accessions. Individuals of var. coloratum showed higher mean genetic distance among them than the ones of var. makarikariense. The PCO biplot and the dendrogram showed some distinction between varieties, although some accessions of var. coloratum were close to var. makarikariense. Variation in var. coloratum agreed with an allogamous reproductive behavior. Alternatively, less variable closely grouped individuals in var. makarikariense, concur with frequent vegetative reproduction by rhizomes of a reduced number of genotypes. Our study showed that genetic variation had different structure between varieties. This difference should be taken into account when planning germplasm management and conservation and in designing breeding programs.
EEA Rafaela
Fil: Armando, Lorena Vanesa. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Almada, Pamela Estefania. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Tomas, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tomas, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description Success in a breeding program is highly dependent on the information regarding genetic variability and its structure of the available germplasm. Particularly in poorly domesticated species, such information is generally scarce. Panicum coloratum L. is a warm-season perennial grass with potential to increase production in agricultural degraded areas. Its two varieties have high genetic polymorphism and morphological overlapping. The goal of the present study was to assess the genetic diversity and intraspecific structure of a collection of P. coloratum using ISSR molecular markers. Eight accessions of var. makarikariense Gooss. and ten from var. coloratum, plus two commercial materials were analyzed. Differences in variability between varieties were evident: polymorphism and percent of variety specific loci were lower (79%P and 10.3%) in var. makarikariense than in var. coloratum (93%P and 30.9%). Analysis of molecular variation (AMOVA) revealed 13.7% (p = 0.001) of variation was attributable to differences between varieties, and 43.8% remained within the accessions. Individuals of var. coloratum showed higher mean genetic distance among them than the ones of var. makarikariense. The PCO biplot and the dendrogram showed some distinction between varieties, although some accessions of var. coloratum were close to var. makarikariense. Variation in var. coloratum agreed with an allogamous reproductive behavior. Alternatively, less variable closely grouped individuals in var. makarikariense, concur with frequent vegetative reproduction by rhizomes of a reduced number of genotypes. Our study showed that genetic variation had different structure between varieties. This difference should be taken into account when planning germplasm management and conservation and in designing breeding programs.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-26T18:18:01Z
2023-04-26T18:18:01Z
2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14588
https://link.springer.com/article/10.1007/s10722-022-01530-3
Armando, L.V., Almada, P.E. & Tomás, M.A. Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding. Genet Resour Crop Evol (2023). https://doi.org/10.1007/s10722-022-01530-3
1573-5109 (electronic)
0925-9864 (print)
https://doi.org/10.1007/s10722-022-01530-3
url http://hdl.handle.net/20.500.12123/14588
https://link.springer.com/article/10.1007/s10722-022-01530-3
https://doi.org/10.1007/s10722-022-01530-3
identifier_str_mv Armando, L.V., Almada, P.E. & Tomás, M.A. Intraspecific differentiation in genetic structure in Panicum coloratum L.: importance for germplasm conservation and breeding. Genet Resour Crop Evol (2023). https://doi.org/10.1007/s10722-022-01530-3
1573-5109 (electronic)
0925-9864 (print)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I142-001/2019-PE-E6-I142-001/AR./Mejoramiento genético de leguminosas y gramíneas forrajeras para incrementar la productividad y la sustentabilidad de los sistemas agropecuarios de la Argentina
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Genetic Resources and Crop Evolution : 1-14. (Published: 02 February 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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