Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia

Autores
Kazimirová, Mária; Hamšíkova, Zuzana; Spitalska, Eva; Minichova, Lenka; Mahrikova, Lenka; Caban, Radoslav; Sprong, Hein; Fonville, Manoj; Schnittger, Leonhard; Kocianova, Elena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Free-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central Europe and many regions around the world. Tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western Slovakia. DNA isolated from these samples was screened for the presence of tick-borne microorganisms by PCR-based methods. Results: Ticks were found to infest all examined ungulate species. The principal infesting tick was Ixodes ricinus, identified on 90.4% of wildlife, and included all developmental stages. Larvae and nymphs of Haemaphysalis concinna were feeding on 9.6% of wildlife. Two specimens of Dermacentor reticulatus were also identified. Ungulates were positive for A. phagocytophilum and Theileria spp. Anaplasma phagocytophilum was found to infect 96.1% of cervids, 88.9% of mouflon, and 28.2% of wild boar, whereas Theileria spp. was detected only in cervids (94.6%). Importantly, a high rate of cervids (89%) showed mixed infections with both these microorganisms. In addition to A. phagocytophilum and Theileria spp., Rickettsia helvetica, R. monacensis, unidentified Rickettsia sp., Coxiella burnetii, “Candidatus Neoehrlichia mikurensis”, Borrelia burgdorferi (s.l.) and Babesia venatorum were identified in engorged I. ricinus. Furthermore, A. phagocytophilum, Babesia spp. and Theileria spp. were detected in engorged H. concinna. Analysis of 16S rRNA and groEL gene sequences revealed the presence of five and two A. phagocytophilum variants, respectively, among which sequences identified in wild boar showed identity to the sequence of the causative agent of human granulocytic anaplasmosis (HGA). Phylogenetic analysis of Theileria 18S rRNA gene sequences amplified from cervids and engorged I. ricinus ticks segregated jointly with sequences of T. capreoli isolates into a moderately supported monophyletic clade. Conclusions: The findings indicate that free-living ungulates are reservoirs for A. phagocytophilum and Theileria spp. and engorged ixodid ticks attached to ungulates are good sentinels for the presence of agents of public and veterinary concern. Further analyses of the A. phagocytophilum genetic variants and Theileria species and their associations with vector ticks and free-living ungulates are required.
Instituto de Patobiología
Fil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Hamsikova, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Spitalska, Eva. Slovak Academy of Sciences. Institute of Virology; Eslovaquia
Fil: Minichova, Lenka. Slovak Academy of Sciences. Institute of Virology; Eslovaquia
Fil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Caban, Radoslav. Bratislava. Široká; Eslovaquia
Fil: Sprong, Hein. National Institute for Public Health and Environment. Laboratory for Zoonoses and Environmental Microbiology; Países Bajos
Fil: Fonville, Manoj. National Institute for Public Health and Environment. Laboratory for Zoonoses and Environmental Microbiology; Países Bajos
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
Fuente
Parasites & Vectors 11 (1) : 495 (Septiembre 2018)
Materia
Wildlife
Tickborne Diseases
Slovak Republic
Vida Silvestre
Enfermedades Transmitidas por Garrapatas
Anaplasma phagocytophilum
Theileria
República Eslovaca
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3773

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spelling Diverse tick-borne microorganisms identified in free-living ungulates in SlovakiaKazimirová, MáriaHamšíkova, ZuzanaSpitalska, EvaMinichova, LenkaMahrikova, LenkaCaban, RadoslavSprong, HeinFonville, ManojSchnittger, LeonhardKocianova, ElenaWildlifeTickborne DiseasesSlovak RepublicVida SilvestreEnfermedades Transmitidas por GarrapatasAnaplasma phagocytophilumTheileriaRepública EslovacaBackground: Free-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central Europe and many regions around the world. Tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western Slovakia. DNA isolated from these samples was screened for the presence of tick-borne microorganisms by PCR-based methods. Results: Ticks were found to infest all examined ungulate species. The principal infesting tick was Ixodes ricinus, identified on 90.4% of wildlife, and included all developmental stages. Larvae and nymphs of Haemaphysalis concinna were feeding on 9.6% of wildlife. Two specimens of Dermacentor reticulatus were also identified. Ungulates were positive for A. phagocytophilum and Theileria spp. Anaplasma phagocytophilum was found to infect 96.1% of cervids, 88.9% of mouflon, and 28.2% of wild boar, whereas Theileria spp. was detected only in cervids (94.6%). Importantly, a high rate of cervids (89%) showed mixed infections with both these microorganisms. In addition to A. phagocytophilum and Theileria spp., Rickettsia helvetica, R. monacensis, unidentified Rickettsia sp., Coxiella burnetii, “Candidatus Neoehrlichia mikurensis”, Borrelia burgdorferi (s.l.) and Babesia venatorum were identified in engorged I. ricinus. Furthermore, A. phagocytophilum, Babesia spp. and Theileria spp. were detected in engorged H. concinna. Analysis of 16S rRNA and groEL gene sequences revealed the presence of five and two A. phagocytophilum variants, respectively, among which sequences identified in wild boar showed identity to the sequence of the causative agent of human granulocytic anaplasmosis (HGA). Phylogenetic analysis of Theileria 18S rRNA gene sequences amplified from cervids and engorged I. ricinus ticks segregated jointly with sequences of T. capreoli isolates into a moderately supported monophyletic clade. Conclusions: The findings indicate that free-living ungulates are reservoirs for A. phagocytophilum and Theileria spp. and engorged ixodid ticks attached to ungulates are good sentinels for the presence of agents of public and veterinary concern. Further analyses of the A. phagocytophilum genetic variants and Theileria species and their associations with vector ticks and free-living ungulates are required.Instituto de PatobiologíaFil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Hamsikova, Zuzana. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Spitalska, Eva. Slovak Academy of Sciences. Institute of Virology; EslovaquiaFil: Minichova, Lenka. Slovak Academy of Sciences. Institute of Virology; EslovaquiaFil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Caban, Radoslav. Bratislava. Široká; EslovaquiaFil: Sprong, Hein. National Institute for Public Health and Environment. Laboratory for Zoonoses and Environmental Microbiology; Países BajosFil: Fonville, Manoj. National Institute for Public Health and Environment. Laboratory for Zoonoses and Environmental Microbiology; Países BajosFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; EslovaquiaBMC2018-11-02T15:35:22Z2018-11-02T15:35:22Z2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-018-3068-1http://hdl.handle.net/20.500.12123/37731756-3305https://doi.org/10.1186/s13071-018-3068-1Parasites & Vectors 11 (1) : 495 (Septiembre 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:28Zoai:localhost:20.500.12123/3773instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:29.308INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
title Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
spellingShingle Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
Kazimirová, Mária
Wildlife
Tickborne Diseases
Slovak Republic
Vida Silvestre
Enfermedades Transmitidas por Garrapatas
Anaplasma phagocytophilum
Theileria
República Eslovaca
title_short Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
title_full Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
title_fullStr Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
title_full_unstemmed Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
title_sort Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia
dc.creator.none.fl_str_mv Kazimirová, Mária
Hamšíkova, Zuzana
Spitalska, Eva
Minichova, Lenka
Mahrikova, Lenka
Caban, Radoslav
Sprong, Hein
Fonville, Manoj
Schnittger, Leonhard
Kocianova, Elena
author Kazimirová, Mária
author_facet Kazimirová, Mária
Hamšíkova, Zuzana
Spitalska, Eva
Minichova, Lenka
Mahrikova, Lenka
Caban, Radoslav
Sprong, Hein
Fonville, Manoj
Schnittger, Leonhard
Kocianova, Elena
author_role author
author2 Hamšíkova, Zuzana
Spitalska, Eva
Minichova, Lenka
Mahrikova, Lenka
Caban, Radoslav
Sprong, Hein
Fonville, Manoj
Schnittger, Leonhard
Kocianova, Elena
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Wildlife
Tickborne Diseases
Slovak Republic
Vida Silvestre
Enfermedades Transmitidas por Garrapatas
Anaplasma phagocytophilum
Theileria
República Eslovaca
topic Wildlife
Tickborne Diseases
Slovak Republic
Vida Silvestre
Enfermedades Transmitidas por Garrapatas
Anaplasma phagocytophilum
Theileria
República Eslovaca
dc.description.none.fl_txt_mv Background: Free-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central Europe and many regions around the world. Tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western Slovakia. DNA isolated from these samples was screened for the presence of tick-borne microorganisms by PCR-based methods. Results: Ticks were found to infest all examined ungulate species. The principal infesting tick was Ixodes ricinus, identified on 90.4% of wildlife, and included all developmental stages. Larvae and nymphs of Haemaphysalis concinna were feeding on 9.6% of wildlife. Two specimens of Dermacentor reticulatus were also identified. Ungulates were positive for A. phagocytophilum and Theileria spp. Anaplasma phagocytophilum was found to infect 96.1% of cervids, 88.9% of mouflon, and 28.2% of wild boar, whereas Theileria spp. was detected only in cervids (94.6%). Importantly, a high rate of cervids (89%) showed mixed infections with both these microorganisms. In addition to A. phagocytophilum and Theileria spp., Rickettsia helvetica, R. monacensis, unidentified Rickettsia sp., Coxiella burnetii, “Candidatus Neoehrlichia mikurensis”, Borrelia burgdorferi (s.l.) and Babesia venatorum were identified in engorged I. ricinus. Furthermore, A. phagocytophilum, Babesia spp. and Theileria spp. were detected in engorged H. concinna. Analysis of 16S rRNA and groEL gene sequences revealed the presence of five and two A. phagocytophilum variants, respectively, among which sequences identified in wild boar showed identity to the sequence of the causative agent of human granulocytic anaplasmosis (HGA). Phylogenetic analysis of Theileria 18S rRNA gene sequences amplified from cervids and engorged I. ricinus ticks segregated jointly with sequences of T. capreoli isolates into a moderately supported monophyletic clade. Conclusions: The findings indicate that free-living ungulates are reservoirs for A. phagocytophilum and Theileria spp. and engorged ixodid ticks attached to ungulates are good sentinels for the presence of agents of public and veterinary concern. Further analyses of the A. phagocytophilum genetic variants and Theileria species and their associations with vector ticks and free-living ungulates are required.
Instituto de Patobiología
Fil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Hamsikova, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Spitalska, Eva. Slovak Academy of Sciences. Institute of Virology; Eslovaquia
Fil: Minichova, Lenka. Slovak Academy of Sciences. Institute of Virology; Eslovaquia
Fil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia
Fil: Caban, Radoslav. Bratislava. Široká; Eslovaquia
Fil: Sprong, Hein. National Institute for Public Health and Environment. Laboratory for Zoonoses and Environmental Microbiology; Países Bajos
Fil: Fonville, Manoj. National Institute for Public Health and Environment. Laboratory for Zoonoses and Environmental Microbiology; Países Bajos
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia
description Background: Free-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central Europe and many regions around the world. Tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western Slovakia. DNA isolated from these samples was screened for the presence of tick-borne microorganisms by PCR-based methods. Results: Ticks were found to infest all examined ungulate species. The principal infesting tick was Ixodes ricinus, identified on 90.4% of wildlife, and included all developmental stages. Larvae and nymphs of Haemaphysalis concinna were feeding on 9.6% of wildlife. Two specimens of Dermacentor reticulatus were also identified. Ungulates were positive for A. phagocytophilum and Theileria spp. Anaplasma phagocytophilum was found to infect 96.1% of cervids, 88.9% of mouflon, and 28.2% of wild boar, whereas Theileria spp. was detected only in cervids (94.6%). Importantly, a high rate of cervids (89%) showed mixed infections with both these microorganisms. In addition to A. phagocytophilum and Theileria spp., Rickettsia helvetica, R. monacensis, unidentified Rickettsia sp., Coxiella burnetii, “Candidatus Neoehrlichia mikurensis”, Borrelia burgdorferi (s.l.) and Babesia venatorum were identified in engorged I. ricinus. Furthermore, A. phagocytophilum, Babesia spp. and Theileria spp. were detected in engorged H. concinna. Analysis of 16S rRNA and groEL gene sequences revealed the presence of five and two A. phagocytophilum variants, respectively, among which sequences identified in wild boar showed identity to the sequence of the causative agent of human granulocytic anaplasmosis (HGA). Phylogenetic analysis of Theileria 18S rRNA gene sequences amplified from cervids and engorged I. ricinus ticks segregated jointly with sequences of T. capreoli isolates into a moderately supported monophyletic clade. Conclusions: The findings indicate that free-living ungulates are reservoirs for A. phagocytophilum and Theileria spp. and engorged ixodid ticks attached to ungulates are good sentinels for the presence of agents of public and veterinary concern. Further analyses of the A. phagocytophilum genetic variants and Theileria species and their associations with vector ticks and free-living ungulates are required.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-02T15:35:22Z
2018-11-02T15:35:22Z
2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-018-3068-1
http://hdl.handle.net/20.500.12123/3773
1756-3305
https://doi.org/10.1186/s13071-018-3068-1
url https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-018-3068-1
http://hdl.handle.net/20.500.12123/3773
https://doi.org/10.1186/s13071-018-3068-1
identifier_str_mv 1756-3305
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Parasites & Vectors 11 (1) : 495 (Septiembre 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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