Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy

Autores
Menzano, Arianna; Tizzani, Paolo; Farber, Marisa Diana; Garcia-Vozmediano, Aitor; Martinelli, Laura; Rossi, Luca; Tomassone, Laura
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the Maritime Alps (northwestern Italy), we collected ticks from vegetation and Alpine ibex (Capra ibex). Ixodes ricinus was the most abundant species in the study area, questing up to 1824 m a.s.l. and infesting 28 out of 72 ibexes. Haemaphysalis punctata, H. sulcata and Dermacentor marginatus were also collected. The abundance of questing ticks significantly decreased with altitude, with beechwoods being the preferred habitat. By PCR, we identified Borrelia burgdorferi s.l. in questing I. ricinus (28.3%; 95%CI: 19.4–38.6) but not in specimens collected from animals. Rickettsia spp. infected both questing (20.6%; 95%CI: 12.9–30.3) and on-host (30.2%; 95%CI: 21.2–40.4) I. ricinus. Anaplasma phagocytophilum was detected in 4.3% (95%CI: 1.2–10.8) of questing I. ricinus and in 45.3% (95%CI: 34.6–56.4) of I. ricinus collected from ibex. Female I. ricinus collected on animals were significantly more infected with A. phagocytophilum than females collected from vegetation (OR = 11.7; 95%CI: 3.8–48.1). By amplifying and sequencing a fragment of the groEL gene, we identified 13 groEL haplotypes, clustering with ecotypes I and II; ecotype I, prevalent in our sample, is considered zoonotic. Our study demonstrates the presence of different tick-borne zoonotic agents in the study area, encompassing a wide altitudinal range, as confirmed by the ticks found on ibex, a typical mountain-dwelling mammal. The results also confirm the altitudinal range expansion of ticks and associated pathogens in the Alps and suggest that Alpine ibex may act as a reservoir for A. phagocytophilum, as do other wild ungulate species.
Instituto de Biotecnología
Fil: Menzano, Arianna. Ente di Gestione delle Aree Protette delle Alpi Marittime; Italia
Fil: Tizzani, Paolo. University of Turin. Department of Veterinary Sciences; Italia
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia-Vozmediano, Aitor. University of Turin. Department of Veterinary Sciences; Italia
Fil: Martinelli, Laura. Ente di Gestione delle Aree Protette delle Alpi Marittime; Italia
Fil: Rossi, Luca. University of Turin. Department of Veterinary Sciences; Italia
Fil: Tomassone, Laura. University of Turin. Department of Veterinary Sciences; Italia
Fuente
Animals 14 (15) : 2251 (Agosto 2024)
Materia
Metastigmata
Anaplasma phagocytophilum
Borrelia burgdorferi
Zoonoses
Tick-borne Diseases
Pathogens
Alps
Italy
Zoonosis
Enfermedad transmitida por Garrapatas
Organismos Patógenos
Capra ibex
Alpes
Italia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/19061
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oai_identifier_str oai:localhost:20.500.12123/19061
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, ItalyMenzano, AriannaTizzani, PaoloFarber, Marisa DianaGarcia-Vozmediano, AitorMartinelli, LauraRossi, LucaTomassone, LauraMetastigmataAnaplasma phagocytophilumBorrelia burgdorferiZoonosesTick-borne DiseasesPathogensAlpsItalyZoonosisEnfermedad transmitida por GarrapatasOrganismos PatógenosCapra ibexAlpesItaliaIn the Maritime Alps (northwestern Italy), we collected ticks from vegetation and Alpine ibex (Capra ibex). Ixodes ricinus was the most abundant species in the study area, questing up to 1824 m a.s.l. and infesting 28 out of 72 ibexes. Haemaphysalis punctata, H. sulcata and Dermacentor marginatus were also collected. The abundance of questing ticks significantly decreased with altitude, with beechwoods being the preferred habitat. By PCR, we identified Borrelia burgdorferi s.l. in questing I. ricinus (28.3%; 95%CI: 19.4–38.6) but not in specimens collected from animals. Rickettsia spp. infected both questing (20.6%; 95%CI: 12.9–30.3) and on-host (30.2%; 95%CI: 21.2–40.4) I. ricinus. Anaplasma phagocytophilum was detected in 4.3% (95%CI: 1.2–10.8) of questing I. ricinus and in 45.3% (95%CI: 34.6–56.4) of I. ricinus collected from ibex. Female I. ricinus collected on animals were significantly more infected with A. phagocytophilum than females collected from vegetation (OR = 11.7; 95%CI: 3.8–48.1). By amplifying and sequencing a fragment of the groEL gene, we identified 13 groEL haplotypes, clustering with ecotypes I and II; ecotype I, prevalent in our sample, is considered zoonotic. Our study demonstrates the presence of different tick-borne zoonotic agents in the study area, encompassing a wide altitudinal range, as confirmed by the ticks found on ibex, a typical mountain-dwelling mammal. The results also confirm the altitudinal range expansion of ticks and associated pathogens in the Alps and suggest that Alpine ibex may act as a reservoir for A. phagocytophilum, as do other wild ungulate species.Instituto de BiotecnologíaFil: Menzano, Arianna. Ente di Gestione delle Aree Protette delle Alpi Marittime; ItaliaFil: Tizzani, Paolo. University of Turin. Department of Veterinary Sciences; ItaliaFil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia-Vozmediano, Aitor. University of Turin. Department of Veterinary Sciences; ItaliaFil: Martinelli, Laura. Ente di Gestione delle Aree Protette delle Alpi Marittime; ItaliaFil: Rossi, Luca. University of Turin. Department of Veterinary Sciences; ItaliaFil: Tomassone, Laura. University of Turin. Department of Veterinary Sciences; ItaliaMDPI2024-08-22T10:31:18Z2024-08-22T10:31:18Z2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/19061https://www.mdpi.com/2076-2615/14/15/22512076-2615https://doi.org/10.3390/ani14152251Animals 14 (15) : 2251 (Agosto 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:46Zoai:localhost:20.500.12123/19061instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:46.66INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
title Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
spellingShingle Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
Menzano, Arianna
Metastigmata
Anaplasma phagocytophilum
Borrelia burgdorferi
Zoonoses
Tick-borne Diseases
Pathogens
Alps
Italy
Zoonosis
Enfermedad transmitida por Garrapatas
Organismos Patógenos
Capra ibex
Alpes
Italia
title_short Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
title_full Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
title_fullStr Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
title_full_unstemmed Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
title_sort Zoonotic tick-borne pathogens in ticks from vegetation and alpine ibex (Capra ibex) in the maritime alps, Italy
dc.creator.none.fl_str_mv Menzano, Arianna
Tizzani, Paolo
Farber, Marisa Diana
Garcia-Vozmediano, Aitor
Martinelli, Laura
Rossi, Luca
Tomassone, Laura
author Menzano, Arianna
author_facet Menzano, Arianna
Tizzani, Paolo
Farber, Marisa Diana
Garcia-Vozmediano, Aitor
Martinelli, Laura
Rossi, Luca
Tomassone, Laura
author_role author
author2 Tizzani, Paolo
Farber, Marisa Diana
Garcia-Vozmediano, Aitor
Martinelli, Laura
Rossi, Luca
Tomassone, Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Metastigmata
Anaplasma phagocytophilum
Borrelia burgdorferi
Zoonoses
Tick-borne Diseases
Pathogens
Alps
Italy
Zoonosis
Enfermedad transmitida por Garrapatas
Organismos Patógenos
Capra ibex
Alpes
Italia
topic Metastigmata
Anaplasma phagocytophilum
Borrelia burgdorferi
Zoonoses
Tick-borne Diseases
Pathogens
Alps
Italy
Zoonosis
Enfermedad transmitida por Garrapatas
Organismos Patógenos
Capra ibex
Alpes
Italia
dc.description.none.fl_txt_mv In the Maritime Alps (northwestern Italy), we collected ticks from vegetation and Alpine ibex (Capra ibex). Ixodes ricinus was the most abundant species in the study area, questing up to 1824 m a.s.l. and infesting 28 out of 72 ibexes. Haemaphysalis punctata, H. sulcata and Dermacentor marginatus were also collected. The abundance of questing ticks significantly decreased with altitude, with beechwoods being the preferred habitat. By PCR, we identified Borrelia burgdorferi s.l. in questing I. ricinus (28.3%; 95%CI: 19.4–38.6) but not in specimens collected from animals. Rickettsia spp. infected both questing (20.6%; 95%CI: 12.9–30.3) and on-host (30.2%; 95%CI: 21.2–40.4) I. ricinus. Anaplasma phagocytophilum was detected in 4.3% (95%CI: 1.2–10.8) of questing I. ricinus and in 45.3% (95%CI: 34.6–56.4) of I. ricinus collected from ibex. Female I. ricinus collected on animals were significantly more infected with A. phagocytophilum than females collected from vegetation (OR = 11.7; 95%CI: 3.8–48.1). By amplifying and sequencing a fragment of the groEL gene, we identified 13 groEL haplotypes, clustering with ecotypes I and II; ecotype I, prevalent in our sample, is considered zoonotic. Our study demonstrates the presence of different tick-borne zoonotic agents in the study area, encompassing a wide altitudinal range, as confirmed by the ticks found on ibex, a typical mountain-dwelling mammal. The results also confirm the altitudinal range expansion of ticks and associated pathogens in the Alps and suggest that Alpine ibex may act as a reservoir for A. phagocytophilum, as do other wild ungulate species.
Instituto de Biotecnología
Fil: Menzano, Arianna. Ente di Gestione delle Aree Protette delle Alpi Marittime; Italia
Fil: Tizzani, Paolo. University of Turin. Department of Veterinary Sciences; Italia
Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia-Vozmediano, Aitor. University of Turin. Department of Veterinary Sciences; Italia
Fil: Martinelli, Laura. Ente di Gestione delle Aree Protette delle Alpi Marittime; Italia
Fil: Rossi, Luca. University of Turin. Department of Veterinary Sciences; Italia
Fil: Tomassone, Laura. University of Turin. Department of Veterinary Sciences; Italia
description In the Maritime Alps (northwestern Italy), we collected ticks from vegetation and Alpine ibex (Capra ibex). Ixodes ricinus was the most abundant species in the study area, questing up to 1824 m a.s.l. and infesting 28 out of 72 ibexes. Haemaphysalis punctata, H. sulcata and Dermacentor marginatus were also collected. The abundance of questing ticks significantly decreased with altitude, with beechwoods being the preferred habitat. By PCR, we identified Borrelia burgdorferi s.l. in questing I. ricinus (28.3%; 95%CI: 19.4–38.6) but not in specimens collected from animals. Rickettsia spp. infected both questing (20.6%; 95%CI: 12.9–30.3) and on-host (30.2%; 95%CI: 21.2–40.4) I. ricinus. Anaplasma phagocytophilum was detected in 4.3% (95%CI: 1.2–10.8) of questing I. ricinus and in 45.3% (95%CI: 34.6–56.4) of I. ricinus collected from ibex. Female I. ricinus collected on animals were significantly more infected with A. phagocytophilum than females collected from vegetation (OR = 11.7; 95%CI: 3.8–48.1). By amplifying and sequencing a fragment of the groEL gene, we identified 13 groEL haplotypes, clustering with ecotypes I and II; ecotype I, prevalent in our sample, is considered zoonotic. Our study demonstrates the presence of different tick-borne zoonotic agents in the study area, encompassing a wide altitudinal range, as confirmed by the ticks found on ibex, a typical mountain-dwelling mammal. The results also confirm the altitudinal range expansion of ticks and associated pathogens in the Alps and suggest that Alpine ibex may act as a reservoir for A. phagocytophilum, as do other wild ungulate species.
publishDate 2024
dc.date.none.fl_str_mv 2024-08-22T10:31:18Z
2024-08-22T10:31:18Z
2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/19061
https://www.mdpi.com/2076-2615/14/15/2251
2076-2615
https://doi.org/10.3390/ani14152251
url http://hdl.handle.net/20.500.12123/19061
https://www.mdpi.com/2076-2615/14/15/2251
https://doi.org/10.3390/ani14152251
identifier_str_mv 2076-2615
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Animals 14 (15) : 2251 (Agosto 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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