Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut

Autores
Arias, Renee S.; Cazon, Luis Ignacio; Massa, Alicia N.; Scheffler, Brian E.; Sobolev, Victor S.; Lamb, Marshall C.; Duke, Mary V.; Simpson, Sheron A.; Conforto, Erica Cinthia; Paredes, Juan Andrés; Soave, Juan H.; Buteler, Mario I.; Rago, Alejandro Mario
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci.
Instituto de Patología Vegetal
Fil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Soave, Juan H. El Carmen S.A. General Cabrera; Argentina
Fil: Buteler, Mario I. El Carmen S.A. General Cabrera; Argentina
Fil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Fungal Genomics & Biology 9 (160) : 1-8 (2019)
Materia
Pathogens
Groundnuts
Fungicides
Fungicidas
Organismos Patógenos
Cacahuete
Arachis hypogaea
Mitochondrial Genome
Carbón del Mani
Thecaphora Frezii
Maní
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10000
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10000
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut SmutArias, Renee S.Cazon, Luis IgnacioMassa, Alicia N.Scheffler, Brian E.Sobolev, Victor S.Lamb, Marshall C.Duke, Mary V.Simpson, Sheron A.Conforto, Erica CinthiaParedes, Juan AndrésSoave, Juan H.Buteler, Mario I.Rago, Alejandro MarioPathogensGroundnutsFungicidesFungicidasOrganismos PatógenosCacahueteArachis hypogaeaMitochondrial GenomeCarbón del ManiThecaphora FreziiManíBackground: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci.Instituto de Patología VegetalFil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados UnidosFil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados UnidosFil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados UnidosFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Soave, Juan H. El Carmen S.A. General Cabrera; ArgentinaFil: Buteler, Mario I. El Carmen S.A. General Cabrera; ArgentinaFil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaLongdom Publishing2021-08-11T11:08:42Z2021-08-11T11:08:42Z2019-08-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10000https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+2165-8056https://doi.org/10.35248/2165-8056.19.9.160Fungal Genomics & Biology 9 (160) : 1-8 (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:18Zoai:localhost:20.500.12123/10000instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:18.631INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
title Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
spellingShingle Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
Arias, Renee S.
Pathogens
Groundnuts
Fungicides
Fungicidas
Organismos Patógenos
Cacahuete
Arachis hypogaea
Mitochondrial Genome
Carbón del Mani
Thecaphora Frezii
Maní
title_short Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
title_full Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
title_fullStr Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
title_full_unstemmed Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
title_sort Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
dc.creator.none.fl_str_mv Arias, Renee S.
Cazon, Luis Ignacio
Massa, Alicia N.
Scheffler, Brian E.
Sobolev, Victor S.
Lamb, Marshall C.
Duke, Mary V.
Simpson, Sheron A.
Conforto, Erica Cinthia
Paredes, Juan Andrés
Soave, Juan H.
Buteler, Mario I.
Rago, Alejandro Mario
author Arias, Renee S.
author_facet Arias, Renee S.
Cazon, Luis Ignacio
Massa, Alicia N.
Scheffler, Brian E.
Sobolev, Victor S.
Lamb, Marshall C.
Duke, Mary V.
Simpson, Sheron A.
Conforto, Erica Cinthia
Paredes, Juan Andrés
Soave, Juan H.
Buteler, Mario I.
Rago, Alejandro Mario
author_role author
author2 Cazon, Luis Ignacio
Massa, Alicia N.
Scheffler, Brian E.
Sobolev, Victor S.
Lamb, Marshall C.
Duke, Mary V.
Simpson, Sheron A.
Conforto, Erica Cinthia
Paredes, Juan Andrés
Soave, Juan H.
Buteler, Mario I.
Rago, Alejandro Mario
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pathogens
Groundnuts
Fungicides
Fungicidas
Organismos Patógenos
Cacahuete
Arachis hypogaea
Mitochondrial Genome
Carbón del Mani
Thecaphora Frezii
Maní
topic Pathogens
Groundnuts
Fungicides
Fungicidas
Organismos Patógenos
Cacahuete
Arachis hypogaea
Mitochondrial Genome
Carbón del Mani
Thecaphora Frezii
Maní
dc.description.none.fl_txt_mv Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci.
Instituto de Patología Vegetal
Fil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Soave, Juan H. El Carmen S.A. General Cabrera; Argentina
Fil: Buteler, Mario I. El Carmen S.A. General Cabrera; Argentina
Fil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-28
2021-08-11T11:08:42Z
2021-08-11T11:08:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10000
https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+
2165-8056
https://doi.org/10.35248/2165-8056.19.9.160
url http://hdl.handle.net/20.500.12123/10000
https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+
https://doi.org/10.35248/2165-8056.19.9.160
identifier_str_mv 2165-8056
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Longdom Publishing
publisher.none.fl_str_mv Longdom Publishing
dc.source.none.fl_str_mv Fungal Genomics & Biology 9 (160) : 1-8 (2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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