Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut
- Autores
- Arias, Renee S.; Cazon, Luis Ignacio; Massa, Alicia N.; Scheffler, Brian E.; Sobolev, Victor S.; Lamb, Marshall C.; Duke, Mary V.; Simpson, Sheron A.; Conforto, Erica Cinthia; Paredes, Juan Andrés; Soave, Juan H.; Buteler, Mario I.; Rago, Alejandro Mario
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci.
Instituto de Patología Vegetal
Fil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados Unidos
Fil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Soave, Juan H. El Carmen S.A. General Cabrera; Argentina
Fil: Buteler, Mario I. El Carmen S.A. General Cabrera; Argentina
Fil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina - Fuente
- Fungal Genomics & Biology 9 (160) : 1-8 (2019)
- Materia
-
Pathogens
Groundnuts
Fungicides
Fungicidas
Organismos Patógenos
Cacahuete
Arachis hypogaea
Mitochondrial Genome
Carbón del Mani
Thecaphora Frezii
Maní - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/10000
Ver los metadatos del registro completo
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Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut SmutArias, Renee S.Cazon, Luis IgnacioMassa, Alicia N.Scheffler, Brian E.Sobolev, Victor S.Lamb, Marshall C.Duke, Mary V.Simpson, Sheron A.Conforto, Erica CinthiaParedes, Juan AndrésSoave, Juan H.Buteler, Mario I.Rago, Alejandro MarioPathogensGroundnutsFungicidesFungicidasOrganismos PatógenosCacahueteArachis hypogaeaMitochondrial GenomeCarbón del ManiThecaphora FreziiManíBackground: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci.Instituto de Patología VegetalFil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados UnidosFil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados UnidosFil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados UnidosFil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados UnidosFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Soave, Juan H. El Carmen S.A. General Cabrera; ArgentinaFil: Buteler, Mario I. El Carmen S.A. General Cabrera; ArgentinaFil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaLongdom Publishing2021-08-11T11:08:42Z2021-08-11T11:08:42Z2019-08-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10000https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+2165-8056https://doi.org/10.35248/2165-8056.19.9.160Fungal Genomics & Biology 9 (160) : 1-8 (2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:00Zoai:localhost:20.500.12123/10000instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:00.377INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
title |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
spellingShingle |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut Arias, Renee S. Pathogens Groundnuts Fungicides Fungicidas Organismos Patógenos Cacahuete Arachis hypogaea Mitochondrial Genome Carbón del Mani Thecaphora Frezii Maní |
title_short |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
title_full |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
title_fullStr |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
title_full_unstemmed |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
title_sort |
Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut |
dc.creator.none.fl_str_mv |
Arias, Renee S. Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario |
author |
Arias, Renee S. |
author_facet |
Arias, Renee S. Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario |
author_role |
author |
author2 |
Cazon, Luis Ignacio Massa, Alicia N. Scheffler, Brian E. Sobolev, Victor S. Lamb, Marshall C. Duke, Mary V. Simpson, Sheron A. Conforto, Erica Cinthia Paredes, Juan Andrés Soave, Juan H. Buteler, Mario I. Rago, Alejandro Mario |
author2_role |
author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Pathogens Groundnuts Fungicides Fungicidas Organismos Patógenos Cacahuete Arachis hypogaea Mitochondrial Genome Carbón del Mani Thecaphora Frezii Maní |
topic |
Pathogens Groundnuts Fungicides Fungicidas Organismos Patógenos Cacahuete Arachis hypogaea Mitochondrial Genome Carbón del Mani Thecaphora Frezii Maní |
dc.description.none.fl_txt_mv |
Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci. Instituto de Patología Vegetal Fil: Arias, Renee S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Cazon, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Massa, Alicia N. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Scheffler, Brian E. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Sobolev, Victor S. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Lamb, Marshall C. USDA-ARS. National Peanut Research Laboratory; Estados Unidos Fil: Duke, Mary V. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Simpson, Sheron A. USDA-ARS. Genomics and Bioinformatics Research Unit; Estados Unidos Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Paredes, Juan Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Soave, Juan H. El Carmen S.A. General Cabrera; Argentina Fil: Buteler, Mario I. El Carmen S.A. General Cabrera; Argentina Fil: Rago, Alejandro Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina |
description |
Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to 35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of that fungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was to provide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) and critical nuclear-encoded genes. Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14 genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomal RNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homing endonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b (cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis (Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidation inhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes in this particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides. Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T. frezii fungicide-target loci. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-28 2021-08-11T11:08:42Z 2021-08-11T11:08:42Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/10000 https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+ 2165-8056 https://doi.org/10.35248/2165-8056.19.9.160 |
url |
http://hdl.handle.net/20.500.12123/10000 https://www.longdom.org/search-results.php?keyword=Mitogenome+and+Nuclear-encoded+ https://doi.org/10.35248/2165-8056.19.9.160 |
identifier_str_mv |
2165-8056 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Longdom Publishing |
publisher.none.fl_str_mv |
Longdom Publishing |
dc.source.none.fl_str_mv |
Fungal Genomics & Biology 9 (160) : 1-8 (2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1842341389608681472 |
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12.623145 |