A simple method to select high superovulatory responder goats

Autores
Bruno Galarraga, María Macarena; Fernandez, Jimena; Lacau Megido, Isabel María; Gonzalez-Bulnes, Antonio; Gibbons, Alejandro Eduardo; Cueto, Marcela Isabel
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance of a high-cost hormonal treatment, as we have previously done in sheep. To this end, we evaluated the number of ovulations obtained in response to the administration of a one-shot eCG treatment and related it with the subsequent ovarian response to a multiple-dose pFSH treatment in 33 goats of the Criolla-Neuquina breed. Goats received a one-shot eCG treatment of 800 IU at the end of a 17-day progestational treatment; 9 days later, started a second 17-day progestational treatment and then received a multiple-dose pFSH treatment on days 15–17 (116 mg pFSH, in six decreasing doses). The number of corpora lutea (CL) per goat was recorded laparoscopically after both hormonal treatments. On day 8 after the second pessary removal, embryos were surgically recovered and classified by quality. Results showed a significant positive correlation between the number of CL obtained in response to the eCG and pFSH treatments (r = 0.41; y = 0.8352x + 6.9906; P < 0.05), although of limited value to select high ovulatory responding goats, due to its low correlation value. Then, goats were grouped into high and low ovulatory responders to the eCG (High ≥9; Low <9 CL) and pFSH treatments (High ≥13; Low <13 CL). After the eCG and pFSH treatments, 60% of the goats maintained their classification as high or low superovulatory responders (expressed as recurrence rate). Significant differences were found in the number of CL (18.2 ± 1.3 vs 9.9 ± 1.3), number of embryos + oocytes (13.5 ± 1.7 vs 7.6 ± 1.7), number of embryos (10.8 ± 1.4 vs 5.1 ± 1.4) and number of Grade 1 and Grade 2 embryos (8.8 ± 1.4 vs 4.3 ± 1.4) between high and low superovulatory responder goats (P < 0.05), while no differences were observed in the number of oocytes and in the recovery of embryos + oocytes, embryos, Grade 1 and Grade 2 embryos and fertilization rates (P > 0.05). In conclusion, the recurrence rate in ovarian response between the one-shot eCG treatment and the multiple-dose pFSH treatment would confirm the existence of an “individual or intrinsic factor” of the donor goat that would respond as a high or low ovulatory responder to superovulatory treatments.
EEA Bariloche
Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Bruno Galarraga, María Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; Argentina
Fil: Gonzalez-Bulnes, Antonio. Universidad Cardenal-Herrera. Facultad de Veterinaria. Departamento de Producción y Sanidad Animal; España
Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Gibbons, Alejandro Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Cueto, Marcela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fuente
Theriogenology 195 : 187-191. (January 2023)
Materia
Caprinos
Reproducción Animal
Superovulación
Métodos
Goats
Animal Reproduction
Superovulation
Methods
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14841
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14841
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A simple method to select high superovulatory responder goatsBruno Galarraga, María MacarenaFernandez, JimenaLacau Megido, Isabel MaríaGonzalez-Bulnes, AntonioGibbons, Alejandro EduardoCueto, Marcela IsabelCaprinosReproducción AnimalSuperovulaciónMétodosGoatsAnimal ReproductionSuperovulationMethodsIn most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance of a high-cost hormonal treatment, as we have previously done in sheep. To this end, we evaluated the number of ovulations obtained in response to the administration of a one-shot eCG treatment and related it with the subsequent ovarian response to a multiple-dose pFSH treatment in 33 goats of the Criolla-Neuquina breed. Goats received a one-shot eCG treatment of 800 IU at the end of a 17-day progestational treatment; 9 days later, started a second 17-day progestational treatment and then received a multiple-dose pFSH treatment on days 15–17 (116 mg pFSH, in six decreasing doses). The number of corpora lutea (CL) per goat was recorded laparoscopically after both hormonal treatments. On day 8 after the second pessary removal, embryos were surgically recovered and classified by quality. Results showed a significant positive correlation between the number of CL obtained in response to the eCG and pFSH treatments (r = 0.41; y = 0.8352x + 6.9906; P < 0.05), although of limited value to select high ovulatory responding goats, due to its low correlation value. Then, goats were grouped into high and low ovulatory responders to the eCG (High ≥9; Low <9 CL) and pFSH treatments (High ≥13; Low <13 CL). After the eCG and pFSH treatments, 60% of the goats maintained their classification as high or low superovulatory responders (expressed as recurrence rate). Significant differences were found in the number of CL (18.2 ± 1.3 vs 9.9 ± 1.3), number of embryos + oocytes (13.5 ± 1.7 vs 7.6 ± 1.7), number of embryos (10.8 ± 1.4 vs 5.1 ± 1.4) and number of Grade 1 and Grade 2 embryos (8.8 ± 1.4 vs 4.3 ± 1.4) between high and low superovulatory responder goats (P < 0.05), while no differences were observed in the number of oocytes and in the recovery of embryos + oocytes, embryos, Grade 1 and Grade 2 embryos and fertilization rates (P > 0.05). In conclusion, the recurrence rate in ovarian response between the one-shot eCG treatment and the multiple-dose pFSH treatment would confirm the existence of an “individual or intrinsic factor” of the donor goat that would respond as a high or low ovulatory responder to superovulatory treatments.EEA BarilocheFil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; ArgentinaFil: Bruno Galarraga, María Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; ArgentinaFil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; ArgentinaFil: Gonzalez-Bulnes, Antonio. Universidad Cardenal-Herrera. Facultad de Veterinaria. Departamento de Producción y Sanidad Animal; EspañaFil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; ArgentinaFil: Gibbons, Alejandro Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; ArgentinaFil: Cueto, Marcela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaElsevier2023-07-31T11:11:49Z2023-07-31T11:11:49Z2023-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14841https://www.sciencedirect.com/science/article/pii/S0093691X220044600093-691X1879-3231https://doi.org/10.1016/j.theriogenology.2022.10.032Theriogenology 195 : 187-191. (January 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001, Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnicoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:12Zoai:localhost:20.500.12123/14841instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:13.399INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A simple method to select high superovulatory responder goats
title A simple method to select high superovulatory responder goats
spellingShingle A simple method to select high superovulatory responder goats
Bruno Galarraga, María Macarena
Caprinos
Reproducción Animal
Superovulación
Métodos
Goats
Animal Reproduction
Superovulation
Methods
title_short A simple method to select high superovulatory responder goats
title_full A simple method to select high superovulatory responder goats
title_fullStr A simple method to select high superovulatory responder goats
title_full_unstemmed A simple method to select high superovulatory responder goats
title_sort A simple method to select high superovulatory responder goats
dc.creator.none.fl_str_mv Bruno Galarraga, María Macarena
Fernandez, Jimena
Lacau Megido, Isabel María
Gonzalez-Bulnes, Antonio
Gibbons, Alejandro Eduardo
Cueto, Marcela Isabel
author Bruno Galarraga, María Macarena
author_facet Bruno Galarraga, María Macarena
Fernandez, Jimena
Lacau Megido, Isabel María
Gonzalez-Bulnes, Antonio
Gibbons, Alejandro Eduardo
Cueto, Marcela Isabel
author_role author
author2 Fernandez, Jimena
Lacau Megido, Isabel María
Gonzalez-Bulnes, Antonio
Gibbons, Alejandro Eduardo
Cueto, Marcela Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Caprinos
Reproducción Animal
Superovulación
Métodos
Goats
Animal Reproduction
Superovulation
Methods
topic Caprinos
Reproducción Animal
Superovulación
Métodos
Goats
Animal Reproduction
Superovulation
Methods
dc.description.none.fl_txt_mv In most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance of a high-cost hormonal treatment, as we have previously done in sheep. To this end, we evaluated the number of ovulations obtained in response to the administration of a one-shot eCG treatment and related it with the subsequent ovarian response to a multiple-dose pFSH treatment in 33 goats of the Criolla-Neuquina breed. Goats received a one-shot eCG treatment of 800 IU at the end of a 17-day progestational treatment; 9 days later, started a second 17-day progestational treatment and then received a multiple-dose pFSH treatment on days 15–17 (116 mg pFSH, in six decreasing doses). The number of corpora lutea (CL) per goat was recorded laparoscopically after both hormonal treatments. On day 8 after the second pessary removal, embryos were surgically recovered and classified by quality. Results showed a significant positive correlation between the number of CL obtained in response to the eCG and pFSH treatments (r = 0.41; y = 0.8352x + 6.9906; P < 0.05), although of limited value to select high ovulatory responding goats, due to its low correlation value. Then, goats were grouped into high and low ovulatory responders to the eCG (High ≥9; Low <9 CL) and pFSH treatments (High ≥13; Low <13 CL). After the eCG and pFSH treatments, 60% of the goats maintained their classification as high or low superovulatory responders (expressed as recurrence rate). Significant differences were found in the number of CL (18.2 ± 1.3 vs 9.9 ± 1.3), number of embryos + oocytes (13.5 ± 1.7 vs 7.6 ± 1.7), number of embryos (10.8 ± 1.4 vs 5.1 ± 1.4) and number of Grade 1 and Grade 2 embryos (8.8 ± 1.4 vs 4.3 ± 1.4) between high and low superovulatory responder goats (P < 0.05), while no differences were observed in the number of oocytes and in the recovery of embryos + oocytes, embryos, Grade 1 and Grade 2 embryos and fertilization rates (P > 0.05). In conclusion, the recurrence rate in ovarian response between the one-shot eCG treatment and the multiple-dose pFSH treatment would confirm the existence of an “individual or intrinsic factor” of the donor goat that would respond as a high or low ovulatory responder to superovulatory treatments.
EEA Bariloche
Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Bruno Galarraga, María Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Fernandez, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; Argentina
Fil: Gonzalez-Bulnes, Antonio. Universidad Cardenal-Herrera. Facultad de Veterinaria. Departamento de Producción y Sanidad Animal; España
Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Gibbons, Alejandro Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Producción Animal; Argentina
Fil: Cueto, Marcela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
description In most female mammals, a common drawback to multiple ovulation embryo transfer programs is the variability in the superovulatory response to the multidose pFSH treatment. The aim of the present study was to identify embryo donor goats based on their response to superovulation before the performance of a high-cost hormonal treatment, as we have previously done in sheep. To this end, we evaluated the number of ovulations obtained in response to the administration of a one-shot eCG treatment and related it with the subsequent ovarian response to a multiple-dose pFSH treatment in 33 goats of the Criolla-Neuquina breed. Goats received a one-shot eCG treatment of 800 IU at the end of a 17-day progestational treatment; 9 days later, started a second 17-day progestational treatment and then received a multiple-dose pFSH treatment on days 15–17 (116 mg pFSH, in six decreasing doses). The number of corpora lutea (CL) per goat was recorded laparoscopically after both hormonal treatments. On day 8 after the second pessary removal, embryos were surgically recovered and classified by quality. Results showed a significant positive correlation between the number of CL obtained in response to the eCG and pFSH treatments (r = 0.41; y = 0.8352x + 6.9906; P < 0.05), although of limited value to select high ovulatory responding goats, due to its low correlation value. Then, goats were grouped into high and low ovulatory responders to the eCG (High ≥9; Low <9 CL) and pFSH treatments (High ≥13; Low <13 CL). After the eCG and pFSH treatments, 60% of the goats maintained their classification as high or low superovulatory responders (expressed as recurrence rate). Significant differences were found in the number of CL (18.2 ± 1.3 vs 9.9 ± 1.3), number of embryos + oocytes (13.5 ± 1.7 vs 7.6 ± 1.7), number of embryos (10.8 ± 1.4 vs 5.1 ± 1.4) and number of Grade 1 and Grade 2 embryos (8.8 ± 1.4 vs 4.3 ± 1.4) between high and low superovulatory responder goats (P < 0.05), while no differences were observed in the number of oocytes and in the recovery of embryos + oocytes, embryos, Grade 1 and Grade 2 embryos and fertilization rates (P > 0.05). In conclusion, the recurrence rate in ovarian response between the one-shot eCG treatment and the multiple-dose pFSH treatment would confirm the existence of an “individual or intrinsic factor” of the donor goat that would respond as a high or low ovulatory responder to superovulatory treatments.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-31T11:11:49Z
2023-07-31T11:11:49Z
2023-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14841
https://www.sciencedirect.com/science/article/pii/S0093691X22004460
0093-691X
1879-3231
https://doi.org/10.1016/j.theriogenology.2022.10.032
url http://hdl.handle.net/20.500.12123/14841
https://www.sciencedirect.com/science/article/pii/S0093691X22004460
https://doi.org/10.1016/j.theriogenology.2022.10.032
identifier_str_mv 0093-691X
1879-3231
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001, Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnico
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Theriogenology 195 : 187-191. (January 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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