A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms

Autores
Vega, Celina Guadalupe; Bok, Marina; Ebinger, Maren; Rocha, Lucía Alejandra; Rivolta, Alejandra Antonella; González Thomas, Valeria; Muntadas, Pilar; D’Aloia, Ricardo; Pinto, Verónica; Parreño, Viviana; Wigdorovitz, Andres
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strategies to prevent severe diarrhea but these are still insufficient. Here we report the field evaluation of a product based on IgY antibodies against group A rotavirus (RVA), coronavirus (CoV), enterotoxigenic Escherichia coli, and Salmonella sp. This product, named IgY DNT, has been designed as a complementary passive immunization strategy to prevent neonatal calf diarrhea. The quality of the product depends on the titers of specific IgY antibodies to each antigen evaluated by ELISA. In the case of the viral antigens, ELISA antibody (Ab) titers are correlated with protection against infection in calves experimentally challenged with RVA and CoV (Bok M, et al., Passive immunity to control bovine coronavirus diarrhea in a dairy herd in Argentina, 2017), (Vega C, et al., Vet Immunol Immunopathol, 142:156–69, 2011), (Vega C, et al., Res Vet Sci, 103:1–10, 2015). To evaluate the efficiency in dairy farms, thirty newborn Holstein calves were randomly assigned to IgY DNT or control groups and treatment initiated after colostrum intake and gut closure. Calves in the IgY DNT group received 20 g of the oral passive treatment in 2 L of milk twice a day during the first 2 weeks of life. Animals were followed until 3 weeks of age and diarrhea due to natural exposure to infectious agents was recorded during all the experimental time. Results: Results demonstrate that the oral administration of IgY DNT during the first 2 weeks of life to newborn calves caused a delay in diarrhea onset and significantly reduced its severity and duration compared with untreated calves. Animals treated with IgY DNT showed a trend towards a delay in RVA infection with significantly shorter duration and virus shedding compared to control calves. Conclusions: This indicates that IgY DNT is an effective product to complement current preventive strategies against neonatal calf diarrhea in dairy farms. Furthermore, to our knowledge, this is the only biological product available for the prevention of virus-associated neonatal calf diarrhea.
Centro de Investigación en Ciencias Veterinarias y Agronómicas
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fil: Ebinger, Maren. El Mangrullo Farm; Argentina
Fil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González Thomas, Valeria. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Muntadas, Pilar. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: D’Aloia, Ricardo. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Pinto, Verónica. Vetanco S.A.; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fuente
BMC Veterinary Research 16 : article number: 264 (2020)
Materia
Ganado de Leche
Enfermedades de los Animales
Respuesta Inmunológica
Anticuerpos
Rota Virus Diarrea de Terneros
Dairy Cattle
Animal Diseases
Immune Response
Antibodies
Calf Diarrhoea Rotavirus
Diarrea Neonatal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7673
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7673
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farmsVega, Celina GuadalupeBok, MarinaEbinger, MarenRocha, Lucía AlejandraRivolta, Alejandra AntonellaGonzález Thomas, ValeriaMuntadas, PilarD’Aloia, RicardoPinto, VerónicaParreño, VivianaWigdorovitz, AndresGanado de LecheEnfermedades de los AnimalesRespuesta InmunológicaAnticuerposRota Virus Diarrea de TernerosDairy CattleAnimal DiseasesImmune ResponseAntibodiesCalf Diarrhoea RotavirusDiarrea NeonatalBackground: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strategies to prevent severe diarrhea but these are still insufficient. Here we report the field evaluation of a product based on IgY antibodies against group A rotavirus (RVA), coronavirus (CoV), enterotoxigenic Escherichia coli, and Salmonella sp. This product, named IgY DNT, has been designed as a complementary passive immunization strategy to prevent neonatal calf diarrhea. The quality of the product depends on the titers of specific IgY antibodies to each antigen evaluated by ELISA. In the case of the viral antigens, ELISA antibody (Ab) titers are correlated with protection against infection in calves experimentally challenged with RVA and CoV (Bok M, et al., Passive immunity to control bovine coronavirus diarrhea in a dairy herd in Argentina, 2017), (Vega C, et al., Vet Immunol Immunopathol, 142:156–69, 2011), (Vega C, et al., Res Vet Sci, 103:1–10, 2015). To evaluate the efficiency in dairy farms, thirty newborn Holstein calves were randomly assigned to IgY DNT or control groups and treatment initiated after colostrum intake and gut closure. Calves in the IgY DNT group received 20 g of the oral passive treatment in 2 L of milk twice a day during the first 2 weeks of life. Animals were followed until 3 weeks of age and diarrhea due to natural exposure to infectious agents was recorded during all the experimental time. Results: Results demonstrate that the oral administration of IgY DNT during the first 2 weeks of life to newborn calves caused a delay in diarrhea onset and significantly reduced its severity and duration compared with untreated calves. Animals treated with IgY DNT showed a trend towards a delay in RVA infection with significantly shorter duration and virus shedding compared to control calves. Conclusions: This indicates that IgY DNT is an effective product to complement current preventive strategies against neonatal calf diarrhea in dairy farms. Furthermore, to our knowledge, this is the only biological product available for the prevention of virus-associated neonatal calf diarrhea.Centro de Investigación en Ciencias Veterinarias y AgronómicasFil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; ArgentinaFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; ArgentinaFil: Ebinger, Maren. El Mangrullo Farm; ArgentinaFil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González Thomas, Valeria. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Muntadas, Pilar. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: D’Aloia, Ricardo. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); ArgentinaFil: Pinto, Verónica. Vetanco S.A.; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; ArgentinaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; ArgentinaBMC2020-08-05T16:54:58Z2020-08-05T16:54:58Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7673https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02476-31746-6148https://doi.org/10.1186/s12917-020-02476-3BMC Veterinary Research 16 : article number: 264 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:51Zoai:localhost:20.500.12123/7673instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:52.308INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
title A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
spellingShingle A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
Vega, Celina Guadalupe
Ganado de Leche
Enfermedades de los Animales
Respuesta Inmunológica
Anticuerpos
Rota Virus Diarrea de Terneros
Dairy Cattle
Animal Diseases
Immune Response
Antibodies
Calf Diarrhoea Rotavirus
Diarrea Neonatal
title_short A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
title_full A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
title_fullStr A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
title_full_unstemmed A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
title_sort A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
dc.creator.none.fl_str_mv Vega, Celina Guadalupe
Bok, Marina
Ebinger, Maren
Rocha, Lucía Alejandra
Rivolta, Alejandra Antonella
González Thomas, Valeria
Muntadas, Pilar
D’Aloia, Ricardo
Pinto, Verónica
Parreño, Viviana
Wigdorovitz, Andres
author Vega, Celina Guadalupe
author_facet Vega, Celina Guadalupe
Bok, Marina
Ebinger, Maren
Rocha, Lucía Alejandra
Rivolta, Alejandra Antonella
González Thomas, Valeria
Muntadas, Pilar
D’Aloia, Ricardo
Pinto, Verónica
Parreño, Viviana
Wigdorovitz, Andres
author_role author
author2 Bok, Marina
Ebinger, Maren
Rocha, Lucía Alejandra
Rivolta, Alejandra Antonella
González Thomas, Valeria
Muntadas, Pilar
D’Aloia, Ricardo
Pinto, Verónica
Parreño, Viviana
Wigdorovitz, Andres
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado de Leche
Enfermedades de los Animales
Respuesta Inmunológica
Anticuerpos
Rota Virus Diarrea de Terneros
Dairy Cattle
Animal Diseases
Immune Response
Antibodies
Calf Diarrhoea Rotavirus
Diarrea Neonatal
topic Ganado de Leche
Enfermedades de los Animales
Respuesta Inmunológica
Anticuerpos
Rota Virus Diarrea de Terneros
Dairy Cattle
Animal Diseases
Immune Response
Antibodies
Calf Diarrhoea Rotavirus
Diarrea Neonatal
dc.description.none.fl_txt_mv Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strategies to prevent severe diarrhea but these are still insufficient. Here we report the field evaluation of a product based on IgY antibodies against group A rotavirus (RVA), coronavirus (CoV), enterotoxigenic Escherichia coli, and Salmonella sp. This product, named IgY DNT, has been designed as a complementary passive immunization strategy to prevent neonatal calf diarrhea. The quality of the product depends on the titers of specific IgY antibodies to each antigen evaluated by ELISA. In the case of the viral antigens, ELISA antibody (Ab) titers are correlated with protection against infection in calves experimentally challenged with RVA and CoV (Bok M, et al., Passive immunity to control bovine coronavirus diarrhea in a dairy herd in Argentina, 2017), (Vega C, et al., Vet Immunol Immunopathol, 142:156–69, 2011), (Vega C, et al., Res Vet Sci, 103:1–10, 2015). To evaluate the efficiency in dairy farms, thirty newborn Holstein calves were randomly assigned to IgY DNT or control groups and treatment initiated after colostrum intake and gut closure. Calves in the IgY DNT group received 20 g of the oral passive treatment in 2 L of milk twice a day during the first 2 weeks of life. Animals were followed until 3 weeks of age and diarrhea due to natural exposure to infectious agents was recorded during all the experimental time. Results: Results demonstrate that the oral administration of IgY DNT during the first 2 weeks of life to newborn calves caused a delay in diarrhea onset and significantly reduced its severity and duration compared with untreated calves. Animals treated with IgY DNT showed a trend towards a delay in RVA infection with significantly shorter duration and virus shedding compared to control calves. Conclusions: This indicates that IgY DNT is an effective product to complement current preventive strategies against neonatal calf diarrhea in dairy farms. Furthermore, to our knowledge, this is the only biological product available for the prevention of virus-associated neonatal calf diarrhea.
Centro de Investigación en Ciencias Veterinarias y Agronómicas
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fil: Ebinger, Maren. El Mangrullo Farm; Argentina
Fil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: González Thomas, Valeria. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Muntadas, Pilar. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: D’Aloia, Ricardo. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina
Fil: Pinto, Verónica. Vetanco S.A.; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina
description Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strategies to prevent severe diarrhea but these are still insufficient. Here we report the field evaluation of a product based on IgY antibodies against group A rotavirus (RVA), coronavirus (CoV), enterotoxigenic Escherichia coli, and Salmonella sp. This product, named IgY DNT, has been designed as a complementary passive immunization strategy to prevent neonatal calf diarrhea. The quality of the product depends on the titers of specific IgY antibodies to each antigen evaluated by ELISA. In the case of the viral antigens, ELISA antibody (Ab) titers are correlated with protection against infection in calves experimentally challenged with RVA and CoV (Bok M, et al., Passive immunity to control bovine coronavirus diarrhea in a dairy herd in Argentina, 2017), (Vega C, et al., Vet Immunol Immunopathol, 142:156–69, 2011), (Vega C, et al., Res Vet Sci, 103:1–10, 2015). To evaluate the efficiency in dairy farms, thirty newborn Holstein calves were randomly assigned to IgY DNT or control groups and treatment initiated after colostrum intake and gut closure. Calves in the IgY DNT group received 20 g of the oral passive treatment in 2 L of milk twice a day during the first 2 weeks of life. Animals were followed until 3 weeks of age and diarrhea due to natural exposure to infectious agents was recorded during all the experimental time. Results: Results demonstrate that the oral administration of IgY DNT during the first 2 weeks of life to newborn calves caused a delay in diarrhea onset and significantly reduced its severity and duration compared with untreated calves. Animals treated with IgY DNT showed a trend towards a delay in RVA infection with significantly shorter duration and virus shedding compared to control calves. Conclusions: This indicates that IgY DNT is an effective product to complement current preventive strategies against neonatal calf diarrhea in dairy farms. Furthermore, to our knowledge, this is the only biological product available for the prevention of virus-associated neonatal calf diarrhea.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-05T16:54:58Z
2020-08-05T16:54:58Z
2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7673
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02476-3
1746-6148
https://doi.org/10.1186/s12917-020-02476-3
url http://hdl.handle.net/20.500.12123/7673
https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02476-3
https://doi.org/10.1186/s12917-020-02476-3
identifier_str_mv 1746-6148
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv BMC Veterinary Research 16 : article number: 264 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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