High genetic diversity and differentiation of the babesia ovis population in Turkey

Autores
Mira, Anabela; Unlu, Ahmet Hakan; Bilgic, Huseyin Bilgin; Bakirci, Serkan; Hacilarlioglu, Selin; Karagenc, Tulin; Carletti, Tamara; Weir, William; Shiels, Brian; Shkap, Varda; Aktas, Munir; Florin‐Christensen, Mónica; Schnittger, Leonhard
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance.
Instituto de Patobiología
Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Unlu, Ahmet Hakan. Van Yuzuncu Yil University. Vocational School of Gevas; Turquía
Fil: Bilgic, Huseyin Bilgin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Bakirci, Serkan. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Hacilarlioglu, Selin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Karagenc, Tulin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Weir, William. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Shiels, Brian. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Shkap, Varda. Kimron Veterinary Institute. Division of Parasitology; Israel
Fil: Aktas, Munir. Firat University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Transboundary and Emerging Diseases 67 (Supl. 2) : 26-35 (Julio 2020)
Materia
Babesia ovis
Diversidad genética (como recurso)
Tipificación de Secuencias Multilocus
Babesiosis
Estructura de la Población
ADN Satélite
Turquía
Ovinos
Caprinos
Genetic diversity (as resource)
Multilocus Sequence Typing
Population Structure
Satellite DNA
Sheep
Goats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7778
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7778
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling High genetic diversity and differentiation of the babesia ovis population in TurkeyMira, AnabelaUnlu, Ahmet HakanBilgic, Huseyin BilginBakirci, SerkanHacilarlioglu, SelinKaragenc, TulinCarletti, TamaraWeir, WilliamShiels, BrianShkap, VardaAktas, MunirFlorin‐Christensen, MónicaSchnittger, LeonhardBabesia ovisDiversidad genética (como recurso)Tipificación de Secuencias MultilocusBabesiosisEstructura de la PoblaciónADN SatéliteTurquíaOvinosCaprinosGenetic diversity (as resource)Multilocus Sequence TypingPopulation StructureSatellite DNASheepGoatsBabesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance.Instituto de PatobiologíaFil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Unlu, Ahmet Hakan. Van Yuzuncu Yil University. Vocational School of Gevas; TurquíaFil: Bilgic, Huseyin Bilgin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; TurquíaFil: Bakirci, Serkan. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; TurquíaFil: Hacilarlioglu, Selin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; TurquíaFil: Karagenc, Tulin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; TurquíaFil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Weir, William. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino UnidoFil: Shiels, Brian. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino UnidoFil: Shkap, Varda. Kimron Veterinary Institute. Division of Parasitology; IsraelFil: Aktas, Munir. Firat University. Faculty of Veterinary Medicine. Department of Parasitology; TurquíaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2020-08-27T16:41:26Z2020-08-27T16:41:26Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7778https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.131741865-1674https://doi.org/10.1111/tbed.13174Transboundary and Emerging Diseases 67 (Supl. 2) : 26-35 (Julio 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:00Zoai:localhost:20.500.12123/7778instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:00.934INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv High genetic diversity and differentiation of the babesia ovis population in Turkey
title High genetic diversity and differentiation of the babesia ovis population in Turkey
spellingShingle High genetic diversity and differentiation of the babesia ovis population in Turkey
Mira, Anabela
Babesia ovis
Diversidad genética (como recurso)
Tipificación de Secuencias Multilocus
Babesiosis
Estructura de la Población
ADN Satélite
Turquía
Ovinos
Caprinos
Genetic diversity (as resource)
Multilocus Sequence Typing
Population Structure
Satellite DNA
Sheep
Goats
title_short High genetic diversity and differentiation of the babesia ovis population in Turkey
title_full High genetic diversity and differentiation of the babesia ovis population in Turkey
title_fullStr High genetic diversity and differentiation of the babesia ovis population in Turkey
title_full_unstemmed High genetic diversity and differentiation of the babesia ovis population in Turkey
title_sort High genetic diversity and differentiation of the babesia ovis population in Turkey
dc.creator.none.fl_str_mv Mira, Anabela
Unlu, Ahmet Hakan
Bilgic, Huseyin Bilgin
Bakirci, Serkan
Hacilarlioglu, Selin
Karagenc, Tulin
Carletti, Tamara
Weir, William
Shiels, Brian
Shkap, Varda
Aktas, Munir
Florin‐Christensen, Mónica
Schnittger, Leonhard
author Mira, Anabela
author_facet Mira, Anabela
Unlu, Ahmet Hakan
Bilgic, Huseyin Bilgin
Bakirci, Serkan
Hacilarlioglu, Selin
Karagenc, Tulin
Carletti, Tamara
Weir, William
Shiels, Brian
Shkap, Varda
Aktas, Munir
Florin‐Christensen, Mónica
Schnittger, Leonhard
author_role author
author2 Unlu, Ahmet Hakan
Bilgic, Huseyin Bilgin
Bakirci, Serkan
Hacilarlioglu, Selin
Karagenc, Tulin
Carletti, Tamara
Weir, William
Shiels, Brian
Shkap, Varda
Aktas, Munir
Florin‐Christensen, Mónica
Schnittger, Leonhard
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Babesia ovis
Diversidad genética (como recurso)
Tipificación de Secuencias Multilocus
Babesiosis
Estructura de la Población
ADN Satélite
Turquía
Ovinos
Caprinos
Genetic diversity (as resource)
Multilocus Sequence Typing
Population Structure
Satellite DNA
Sheep
Goats
topic Babesia ovis
Diversidad genética (como recurso)
Tipificación de Secuencias Multilocus
Babesiosis
Estructura de la Población
ADN Satélite
Turquía
Ovinos
Caprinos
Genetic diversity (as resource)
Multilocus Sequence Typing
Population Structure
Satellite DNA
Sheep
Goats
dc.description.none.fl_txt_mv Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance.
Instituto de Patobiología
Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Unlu, Ahmet Hakan. Van Yuzuncu Yil University. Vocational School of Gevas; Turquía
Fil: Bilgic, Huseyin Bilgin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Bakirci, Serkan. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Hacilarlioglu, Selin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Karagenc, Tulin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Weir, William. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Shiels, Brian. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido
Fil: Shkap, Varda. Kimron Veterinary Institute. Division of Parasitology; Israel
Fil: Aktas, Munir. Firat University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-27T16:41:26Z
2020-08-27T16:41:26Z
2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7778
https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13174
1865-1674
https://doi.org/10.1111/tbed.13174
url http://hdl.handle.net/20.500.12123/7778
https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13174
https://doi.org/10.1111/tbed.13174
identifier_str_mv 1865-1674
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Transboundary and Emerging Diseases 67 (Supl. 2) : 26-35 (Julio 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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