Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation

Autores
Flores, Daniela Agustina; Minichiello, Y.; Araujo, F. R.; Shkap, V.; Benítez, D.; Echaide, Ignacio Eduardo; Rolls, P.; Mosqueda, J.; Pacheco, G. M.; Petterson, M.; Jacobsen, Monica Ofelia; Schnittger, Leonhard
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and minisatellite markers was used to estimate population genetic parameters of eighteen parasite isolates originating from different continents, countries and geographic regions including North America (Mexico, USA), South America (Argentina, Brazil), the Middle East (Israel) and Australia. For eleven of the eighteen isolates, a unique haplotype was inferred suggesting selection of a single genotype by either in vitro cultivation or amplification in splenectomized calves. Furthermore, a high genetic diversity (H = 0.780) over all marker loci was estimated. Linkage disequilibrium was observed in the total study group but also in sample subgroups from the Americas, Brazil, and Israel and Australia. In contrast, corresponding to their more confined geographic origin, samples from Israel and Argentina were each found to be in equilibrium suggestive of random mating and frequent genetic exchange. The genetic differentiation (FST) of the total study group over all nineteen loci was estimated by analysis of variance (Θ) and Nei's estimation of heterozygosity (GST') as 0.296 and 0.312, respectively. Thus, about 30% of the genetic diversity of the parasite population is associated with genetic differences between parasite isolates sampled from the different geographic regions. The pairwise similarity of multilocus genotypes (MLGs) was assessed and a neighbour-joining dendrogram generated. MLGs were found to cluster according to the country/continent of origin of isolates, but did not distinguish the attenuated from the pathogenic parasite state. The distant geographic origin of the isolates studied allows an initial glimpse into the large extent of genetic diversity and differentiation of the B. bovis population on a global scale.
Fil: Flores, Daniela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Minichiello, Y.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Araujo, F. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Shkap, V.. Kimron Veterinary Institute Israel; Israel
Fil: Benítez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Rolls, P.. Tick Fever Centre; Australia
Fil: Mosqueda, J.. Universidad Autónoma de Querétaro; México
Fil: Pacheco, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Petterson, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Jacobsen, Monica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Materia
BABESIA BOVIS
BOVINE BABESIOSIS
GENETIC DIVERSITY
MULTILOCUS TYPING
POPULATION STRUCTURE
SATELLITE MARKERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88833
Acceder
id CONICETDig_bc6ebb0d6aae7a13b8209b5ba4e0e227
oai_identifier_str oai:ri.conicet.gov.ar:11336/88833
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis MetapopulationFlores, Daniela AgustinaMinichiello, Y.Araujo, F. R.Shkap, V.Benítez, D.Echaide, Ignacio EduardoRolls, P.Mosqueda, J.Pacheco, G. M.Petterson, M.Jacobsen, Monica OfeliaSchnittger, LeonhardBABESIA BOVISBOVINE BABESIOSISGENETIC DIVERSITYMULTILOCUS TYPINGPOPULATION STRUCTURESATELLITE MARKERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and minisatellite markers was used to estimate population genetic parameters of eighteen parasite isolates originating from different continents, countries and geographic regions including North America (Mexico, USA), South America (Argentina, Brazil), the Middle East (Israel) and Australia. For eleven of the eighteen isolates, a unique haplotype was inferred suggesting selection of a single genotype by either in vitro cultivation or amplification in splenectomized calves. Furthermore, a high genetic diversity (H = 0.780) over all marker loci was estimated. Linkage disequilibrium was observed in the total study group but also in sample subgroups from the Americas, Brazil, and Israel and Australia. In contrast, corresponding to their more confined geographic origin, samples from Israel and Argentina were each found to be in equilibrium suggestive of random mating and frequent genetic exchange. The genetic differentiation (FST) of the total study group over all nineteen loci was estimated by analysis of variance (Θ) and Nei's estimation of heterozygosity (GST') as 0.296 and 0.312, respectively. Thus, about 30% of the genetic diversity of the parasite population is associated with genetic differences between parasite isolates sampled from the different geographic regions. The pairwise similarity of multilocus genotypes (MLGs) was assessed and a neighbour-joining dendrogram generated. MLGs were found to cluster according to the country/continent of origin of isolates, but did not distinguish the attenuated from the pathogenic parasite state. The distant geographic origin of the isolates studied allows an initial glimpse into the large extent of genetic diversity and differentiation of the B. bovis population on a global scale.Fil: Flores, Daniela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Minichiello, Y.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Araujo, F. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Shkap, V.. Kimron Veterinary Institute Israel; IsraelFil: Benítez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Rolls, P.. Tick Fever Centre; AustraliaFil: Mosqueda, J.. Universidad Autónoma de Querétaro; MéxicoFil: Pacheco, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Petterson, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Jacobsen, Monica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaWiley Blackwell Publishing, Inc2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88833Flores, Daniela Agustina; Minichiello, Y.; Araujo, F. R.; Shkap, V.; Benítez, D.; et al.; Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation; Wiley Blackwell Publishing, Inc; Transboundary And Emerging Diseases; 60; SUPPL.2; 11-2013; 131-1361865-1674CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/tbed.12121info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.12121info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:50Zoai:ri.conicet.gov.ar:11336/88833instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:50.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
title Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
spellingShingle Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
Flores, Daniela Agustina
BABESIA BOVIS
BOVINE BABESIOSIS
GENETIC DIVERSITY
MULTILOCUS TYPING
POPULATION STRUCTURE
SATELLITE MARKERS
title_short Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
title_full Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
title_fullStr Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
title_full_unstemmed Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
title_sort Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation
dc.creator.none.fl_str_mv Flores, Daniela Agustina
Minichiello, Y.
Araujo, F. R.
Shkap, V.
Benítez, D.
Echaide, Ignacio Eduardo
Rolls, P.
Mosqueda, J.
Pacheco, G. M.
Petterson, M.
Jacobsen, Monica Ofelia
Schnittger, Leonhard
author Flores, Daniela Agustina
author_facet Flores, Daniela Agustina
Minichiello, Y.
Araujo, F. R.
Shkap, V.
Benítez, D.
Echaide, Ignacio Eduardo
Rolls, P.
Mosqueda, J.
Pacheco, G. M.
Petterson, M.
Jacobsen, Monica Ofelia
Schnittger, Leonhard
author_role author
author2 Minichiello, Y.
Araujo, F. R.
Shkap, V.
Benítez, D.
Echaide, Ignacio Eduardo
Rolls, P.
Mosqueda, J.
Pacheco, G. M.
Petterson, M.
Jacobsen, Monica Ofelia
Schnittger, Leonhard
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BABESIA BOVIS
BOVINE BABESIOSIS
GENETIC DIVERSITY
MULTILOCUS TYPING
POPULATION STRUCTURE
SATELLITE MARKERS
topic BABESIA BOVIS
BOVINE BABESIOSIS
GENETIC DIVERSITY
MULTILOCUS TYPING
POPULATION STRUCTURE
SATELLITE MARKERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and minisatellite markers was used to estimate population genetic parameters of eighteen parasite isolates originating from different continents, countries and geographic regions including North America (Mexico, USA), South America (Argentina, Brazil), the Middle East (Israel) and Australia. For eleven of the eighteen isolates, a unique haplotype was inferred suggesting selection of a single genotype by either in vitro cultivation or amplification in splenectomized calves. Furthermore, a high genetic diversity (H = 0.780) over all marker loci was estimated. Linkage disequilibrium was observed in the total study group but also in sample subgroups from the Americas, Brazil, and Israel and Australia. In contrast, corresponding to their more confined geographic origin, samples from Israel and Argentina were each found to be in equilibrium suggestive of random mating and frequent genetic exchange. The genetic differentiation (FST) of the total study group over all nineteen loci was estimated by analysis of variance (Θ) and Nei's estimation of heterozygosity (GST') as 0.296 and 0.312, respectively. Thus, about 30% of the genetic diversity of the parasite population is associated with genetic differences between parasite isolates sampled from the different geographic regions. The pairwise similarity of multilocus genotypes (MLGs) was assessed and a neighbour-joining dendrogram generated. MLGs were found to cluster according to the country/continent of origin of isolates, but did not distinguish the attenuated from the pathogenic parasite state. The distant geographic origin of the isolates studied allows an initial glimpse into the large extent of genetic diversity and differentiation of the B. bovis population on a global scale.
Fil: Flores, Daniela Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Minichiello, Y.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Araujo, F. R.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Shkap, V.. Kimron Veterinary Institute Israel; Israel
Fil: Benítez, D.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Corrientes. Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Rolls, P.. Tick Fever Centre; Australia
Fil: Mosqueda, J.. Universidad Autónoma de Querétaro; México
Fil: Pacheco, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Petterson, M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Jacobsen, Monica Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
description Babesia bovis is a tick-transmitted haemoprotozoan and a causative agent of bovine babesiosis, a cattle disease that causes significant economic loss in tropical and subtropical regions. A panel of nineteen micro- and minisatellite markers was used to estimate population genetic parameters of eighteen parasite isolates originating from different continents, countries and geographic regions including North America (Mexico, USA), South America (Argentina, Brazil), the Middle East (Israel) and Australia. For eleven of the eighteen isolates, a unique haplotype was inferred suggesting selection of a single genotype by either in vitro cultivation or amplification in splenectomized calves. Furthermore, a high genetic diversity (H = 0.780) over all marker loci was estimated. Linkage disequilibrium was observed in the total study group but also in sample subgroups from the Americas, Brazil, and Israel and Australia. In contrast, corresponding to their more confined geographic origin, samples from Israel and Argentina were each found to be in equilibrium suggestive of random mating and frequent genetic exchange. The genetic differentiation (FST) of the total study group over all nineteen loci was estimated by analysis of variance (Θ) and Nei's estimation of heterozygosity (GST') as 0.296 and 0.312, respectively. Thus, about 30% of the genetic diversity of the parasite population is associated with genetic differences between parasite isolates sampled from the different geographic regions. The pairwise similarity of multilocus genotypes (MLGs) was assessed and a neighbour-joining dendrogram generated. MLGs were found to cluster according to the country/continent of origin of isolates, but did not distinguish the attenuated from the pathogenic parasite state. The distant geographic origin of the isolates studied allows an initial glimpse into the large extent of genetic diversity and differentiation of the B. bovis population on a global scale.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88833
Flores, Daniela Agustina; Minichiello, Y.; Araujo, F. R.; Shkap, V.; Benítez, D.; et al.; Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation; Wiley Blackwell Publishing, Inc; Transboundary And Emerging Diseases; 60; SUPPL.2; 11-2013; 131-136
1865-1674
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88833
identifier_str_mv Flores, Daniela Agustina; Minichiello, Y.; Araujo, F. R.; Shkap, V.; Benítez, D.; et al.; Evidence for Extensive Genetic Diversity and Substructuring of the Babesia bovis Metapopulation; Wiley Blackwell Publishing, Inc; Transboundary And Emerging Diseases; 60; SUPPL.2; 11-2013; 131-136
1865-1674
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/tbed.12121
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.12121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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