An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii

Autores
Carpane, Pablo; Melcher, Ulrich; Wayadande, Astri; Gimenez Pecci, Maria De La Paz; Laguna, Irma Graciela; Dolezal, William; Fletcher, Jacqueline
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.
Instituto de Patología Vegetal
Fil: Carpane, Pablo. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos
Fil: Melcher, Ulrich. Oklahoma State University. Department of Biochemistry and Molecular Biology; Estados Unidos
Fil: Wayadande, Astri. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos
Fil: Giménez Pecci, María de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Dolezal, William. Pioneer Hi-Bred International; Estados Unidos
Fil: Fletcher, Jacqueline. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos
Fuente
Phytopathology 103 (2) : 129-134 (February 2013)
Materia
Enfermedades de las Plantas
Spiroplasma
Variación Genética
Maíz
Polimorfismo
Plant Diseases
Genetic Variation
Maize
Polymorphism
Spiroplasma kunkelii
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4264
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spelling An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkeliiCarpane, PabloMelcher, UlrichWayadande, AstriGimenez Pecci, Maria De La PazLaguna, Irma GracielaDolezal, WilliamFletcher, JacquelineEnfermedades de las PlantasSpiroplasmaVariación GenéticaMaízPolimorfismoPlant DiseasesGenetic VariationMaizePolymorphismSpiroplasma kunkeliiCorn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.Instituto de Patología VegetalFil: Carpane, Pablo. Oklahoma State University. Department of Entomology and Plant Pathology; Estados UnidosFil: Melcher, Ulrich. Oklahoma State University. Department of Biochemistry and Molecular Biology; Estados UnidosFil: Wayadande, Astri. Oklahoma State University. Department of Entomology and Plant Pathology; Estados UnidosFil: Giménez Pecci, María de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Dolezal, William. Pioneer Hi-Bred International; Estados UnidosFil: Fletcher, Jacqueline. Oklahoma State University. Department of Entomology and Plant Pathology; Estados UnidosAmerican Phytopathological Society2019-01-14T17:30:09Z2019-01-14T17:30:09Z2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-Rhttp://hdl.handle.net/20.500.12123/42640031-949X1943-7684https://doi.org/10.1094/PHYTO-07-12-0158-RPhytopathology 103 (2) : 129-134 (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-18T10:07:26Zoai:localhost:20.500.12123/4264instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:26.858INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
title An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
spellingShingle An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
Carpane, Pablo
Enfermedades de las Plantas
Spiroplasma
Variación Genética
Maíz
Polimorfismo
Plant Diseases
Genetic Variation
Maize
Polymorphism
Spiroplasma kunkelii
title_short An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
title_full An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
title_fullStr An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
title_full_unstemmed An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
title_sort An analysis of the genomic variability of the phytopathogenic mollicute Spiroplasma kunkelii
dc.creator.none.fl_str_mv Carpane, Pablo
Melcher, Ulrich
Wayadande, Astri
Gimenez Pecci, Maria De La Paz
Laguna, Irma Graciela
Dolezal, William
Fletcher, Jacqueline
author Carpane, Pablo
author_facet Carpane, Pablo
Melcher, Ulrich
Wayadande, Astri
Gimenez Pecci, Maria De La Paz
Laguna, Irma Graciela
Dolezal, William
Fletcher, Jacqueline
author_role author
author2 Melcher, Ulrich
Wayadande, Astri
Gimenez Pecci, Maria De La Paz
Laguna, Irma Graciela
Dolezal, William
Fletcher, Jacqueline
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de las Plantas
Spiroplasma
Variación Genética
Maíz
Polimorfismo
Plant Diseases
Genetic Variation
Maize
Polymorphism
Spiroplasma kunkelii
topic Enfermedades de las Plantas
Spiroplasma
Variación Genética
Maíz
Polimorfismo
Plant Diseases
Genetic Variation
Maize
Polymorphism
Spiroplasma kunkelii
dc.description.none.fl_txt_mv Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.
Instituto de Patología Vegetal
Fil: Carpane, Pablo. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos
Fil: Melcher, Ulrich. Oklahoma State University. Department of Biochemistry and Molecular Biology; Estados Unidos
Fil: Wayadande, Astri. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos
Fil: Giménez Pecci, María de la Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Dolezal, William. Pioneer Hi-Bred International; Estados Unidos
Fil: Fletcher, Jacqueline. Oklahoma State University. Department of Entomology and Plant Pathology; Estados Unidos
description Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
2019-01-14T17:30:09Z
2019-01-14T17:30:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-R
http://hdl.handle.net/20.500.12123/4264
0031-949X
1943-7684
https://doi.org/10.1094/PHYTO-07-12-0158-R
url https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-R
http://hdl.handle.net/20.500.12123/4264
https://doi.org/10.1094/PHYTO-07-12-0158-R
identifier_str_mv 0031-949X
1943-7684
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Phytopathology 103 (2) : 129-134 (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1843609175097081856
score 13.001348

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