An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii

Autores
Carpane, Pablo; Melcher, Ulrich; Wayadande, Astri; Giménez, María de la Paz; Laguna, Irma Graciela; Dolezal, William; Fletcher, Jacqueline
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.
Fil: Carpane, Pablo. Oklahoma State University; Estados Unidos
Fil: Melcher, Ulrich. Oklahoma State University; Estados Unidos
Fil: Wayadande, Astri. Oklahoma State University; Estados Unidos
Fil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dolezal, William. Pioneer Hi-Bred International; Estados Unidos
Fil: Fletcher, Jacqueline. Oklahoma State University; Estados Unidos
Materia
GENOMIC
SPIROPLASMA
KUNKELII
MOLLICUTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22794
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22794
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkeliiCarpane, PabloMelcher, UlrichWayadande, AstriGiménez, María de la PazLaguna, Irma GracielaDolezal, WilliamFletcher, JacquelineGENOMICSPIROPLASMAKUNKELIIMOLLICUTEhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.Fil: Carpane, Pablo. Oklahoma State University; Estados UnidosFil: Melcher, Ulrich. Oklahoma State University; Estados UnidosFil: Wayadande, Astri. Oklahoma State University; Estados UnidosFil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dolezal, William. Pioneer Hi-Bred International; Estados UnidosFil: Fletcher, Jacqueline. Oklahoma State University; Estados UnidosAmerican Phytopathological Society2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22794Carpane, Pablo; Melcher, Ulrich; Wayadande, Astri; Giménez, María de la Paz; Laguna, Irma Graciela; et al.; An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii; American Phytopathological Society; Phytopathology; 103; 2; 2-2013; 129-1340031-949XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-07-12-0158-Rinfo:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:53:34Zoai:ri.conicet.gov.ar:11336/22794instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:53:35.084CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
title An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
spellingShingle An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
Carpane, Pablo
GENOMIC
SPIROPLASMA
KUNKELII
MOLLICUTE
title_short An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
title_full An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
title_fullStr An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
title_full_unstemmed An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
title_sort An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii
dc.creator.none.fl_str_mv Carpane, Pablo
Melcher, Ulrich
Wayadande, Astri
Giménez, María de la Paz
Laguna, Irma Graciela
Dolezal, William
Fletcher, Jacqueline
author Carpane, Pablo
author_facet Carpane, Pablo
Melcher, Ulrich
Wayadande, Astri
Giménez, María de la Paz
Laguna, Irma Graciela
Dolezal, William
Fletcher, Jacqueline
author_role author
author2 Melcher, Ulrich
Wayadande, Astri
Giménez, María de la Paz
Laguna, Irma Graciela
Dolezal, William
Fletcher, Jacqueline
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv GENOMIC
SPIROPLASMA
KUNKELII
MOLLICUTE
topic GENOMIC
SPIROPLASMA
KUNKELII
MOLLICUTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.
Fil: Carpane, Pablo. Oklahoma State University; Estados Unidos
Fil: Melcher, Ulrich. Oklahoma State University; Estados Unidos
Fil: Wayadande, Astri. Oklahoma State University; Estados Unidos
Fil: Giménez, María de la Paz. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronomicas. Instituto de Fitopatología y Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dolezal, William. Pioneer Hi-Bred International; Estados Unidos
Fil: Fletcher, Jacqueline. Oklahoma State University; Estados Unidos
description Corn stunt disease has become a factor limiting maize production in some areas of the Americas in recent years. Although resistant maize genotypes have been developed in the past, this resistance has been unstable over time or in some geographical locations. To better understand disease components that could affect the stability of host resistance, we assessed the genome variability of the etiologic agent, Spiroplasma kunkelii. Isolates were obtained from a number of areas, and characterized molecularly by amplification of several regions of the spiroplasma chromosome and sequencing of specific gene fragments. The degree of polymorphism between isolates of different geographic origins was low, and the level of genomic variability was similar within isolates of different countries. Polymorphism among isolates was found in viral insertions and in the sequence of Skarp, a gene that encodes a membrane protein implicated in attachment to insect cells. The results suggest that the genome composition of this species is highly conserved among isolates. Hence, it is unlikely that the instability of maize resistance is due to generation of new pathotypes of S. kunkelii. Instead, other components of this complex pathosystem could account for the breakdown of resistance.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22794
Carpane, Pablo; Melcher, Ulrich; Wayadande, Astri; Giménez, María de la Paz; Laguna, Irma Graciela; et al.; An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii; American Phytopathological Society; Phytopathology; 103; 2; 2-2013; 129-134
0031-949X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22794
identifier_str_mv Carpane, Pablo; Melcher, Ulrich; Wayadande, Astri; Giménez, María de la Paz; Laguna, Irma Graciela; et al.; An Analysis of the Genomic Variability of the Phytopathogenic Mollicute Spiroplasma kunkelii; American Phytopathological Society; Phytopathology; 103; 2; 2-2013; 129-134
0031-949X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1094/PHYTO-07-12-0158-R
info:eu-repo/semantics/altIdentifier/url/http://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-12-0158-R
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606179570253824
score 13.001348

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