Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates

Autores
Piccinni, Florencia Elizabeth; Ontañon, Ornella Mailén; Ghio, Silvina; Sauka, Diego Hernan; Talia, Paola Mónica; Rivarola, Maximo Lisandro; Valacco, María Pía; Campos, Eleonora
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.
Instituto de Biotecnología
Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of applied microbiology 126 (3) : 811-825. (March 2019)
Materia
Cellulomonas
Glicosiltransferasas
Lignocelulosa
Glycosyltransferases
Lignocellulose
Secretome
Cazymes
GH10
Glucanases
Glycosyl Hydrolases
Xylanases
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4769
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4769
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substratesPiccinni, Florencia ElizabethOntañon, Ornella MailénGhio, SilvinaSauka, Diego HernanTalia, Paola MónicaRivarola, Maximo LisandroValacco, María PíaCampos, EleonoraCellulomonasGlicosiltransferasasLignocelulosaGlycosyltransferasesLignocelluloseSecretomeCazymesGH10GlucanasesGlycosyl HydrolasesXylanasesAims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.Instituto de BiotecnologíaFil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley; Society for Applied Microbiology2019-03-28T13:15:20Z2019-03-28T13:15:20Z2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4769https://onlinelibrary.wiley.com/doi/full/10.1111/jam.141761364-5072https://doi.org/10.1111/jam.14176Journal of applied microbiology 126 (3) : 811-825. (March 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-11T10:22:59Zoai:localhost:20.500.12123/4769instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:59.998INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
spellingShingle Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
Piccinni, Florencia Elizabeth
Cellulomonas
Glicosiltransferasas
Lignocelulosa
Glycosyltransferases
Lignocellulose
Secretome
Cazymes
GH10
Glucanases
Glycosyl Hydrolases
Xylanases
title_short Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_full Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_fullStr Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_full_unstemmed Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
title_sort Secretome profile of Cellulomonas sp. B6 growing on lignocellulosic substrates
dc.creator.none.fl_str_mv Piccinni, Florencia Elizabeth
Ontañon, Ornella Mailén
Ghio, Silvina
Sauka, Diego Hernan
Talia, Paola Mónica
Rivarola, Maximo Lisandro
Valacco, María Pía
Campos, Eleonora
author Piccinni, Florencia Elizabeth
author_facet Piccinni, Florencia Elizabeth
Ontañon, Ornella Mailén
Ghio, Silvina
Sauka, Diego Hernan
Talia, Paola Mónica
Rivarola, Maximo Lisandro
Valacco, María Pía
Campos, Eleonora
author_role author
author2 Ontañon, Ornella Mailén
Ghio, Silvina
Sauka, Diego Hernan
Talia, Paola Mónica
Rivarola, Maximo Lisandro
Valacco, María Pía
Campos, Eleonora
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cellulomonas
Glicosiltransferasas
Lignocelulosa
Glycosyltransferases
Lignocellulose
Secretome
Cazymes
GH10
Glucanases
Glycosyl Hydrolases
Xylanases
topic Cellulomonas
Glicosiltransferasas
Lignocelulosa
Glycosyltransferases
Lignocellulose
Secretome
Cazymes
GH10
Glucanases
Glycosyl Hydrolases
Xylanases
dc.description.none.fl_txt_mv Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.
Instituto de Biotecnología
Fil: Piccinni, Florencia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ontañon, Ornella Mailén. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aims: Lignocellulosic biomass deconstruction is a bottleneck for obtaining biofuels and value‐added products. Our main goal was to characterize the secretome of a novel isolate, Cellulomonas sp. B6, when grown on residual biomass for the formulation of cost‐efficient enzymatic cocktails. Methods and Results: We identified 205 potential CAZymes in the genome of Cellulomonas sp. B6, 91 of which were glycoside hydrolases (GH). By secretome analysis of supernatants from cultures in either extruded wheat straw (EWS), grinded sugar cane straw (SCR) or carboxymethylcellulose (CMC), we identified which proteins played a role in lignocellulose deconstruction. Growth on CMC resulted in the secretion of two exoglucanases (GH6 and GH48) and two GH10 xylanases, while growth on SCR or EWS resulted in the identification of a diversity of CAZymes. From the 32 GHs predicted to be secreted, 22 were identified in supernatants from EWS and/or SCR cultures, including endo‐ and exoglucanases, xylanases, a xyloglucanase, an arabinofuranosidase/β‐xylosidase, a β‐glucosidase and an AA10. Surprisingly, among the xylanases, seven were GH10. Conclusions: Growth of Cellulomonas sp. B6 on lignocellulosic biomass induced the secretion of a diverse repertoire of CAZymes. Significance and Impact of the Study: Cellulomonas sp. B6 could serve as a source of lignocellulose‐degrading enzymes applicable to bioprocessing and biotechnological industries.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
2019-03-28T13:15:20Z
2019-03-28T13:15:20Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4769
https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176
1364-5072
https://doi.org/10.1111/jam.14176
url http://hdl.handle.net/20.500.12123/4769
https://onlinelibrary.wiley.com/doi/full/10.1111/jam.14176
https://doi.org/10.1111/jam.14176
identifier_str_mv 1364-5072
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley; Society for Applied Microbiology
publisher.none.fl_str_mv Wiley; Society for Applied Microbiology
dc.source.none.fl_str_mv Journal of applied microbiology 126 (3) : 811-825. (March 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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