Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio

Autores
Estravis Barcala, Maximiliano; Herr, Katrin; Marchelli, Paula; Ziegenhagen, Birgit; Arana, María Veronica; Bellora Pereyra, Nicolás
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underly ing the heat stress response is therefore crucial for the development of conservation strate gies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first tran scriptomic resource for the species. We also studied the changes in protein-coding tran scripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the exis tence of shared molecular features of heat stress responses across angiosperms, and iden tify genes of potential biotechnological application.
EEA Bariloche
Fil: Estravis-Barcala, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Institute of Nuclear Technologies for Health; Argentina.
Fil: Herr, Katrin. Philipps-Universität Marburg; Alemania.
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ziegenhagen, Birgit. Philipps-Universität Marburg; Alemania
Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Arana, María Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Bellora Pereyra, Nicolás. Institute of Nuclear Technologies for Health (INTECNUS), National Scientific and Research Council (CONICET); Argentina.
Fuente
PLOS One 16 (3) : e0246615 (2021)
Materia
Calentamiento Global
Fisiología Vegetal
Estres Térmico
Genotipos
Factores de Transcripción
Secuencia de ARN
Global Warming
Plant Physiology
Heat Stress
Genotypes
Transcription Factors
RNA Secuence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14909
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oai_identifier_str oai:localhost:20.500.12123/14909
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network_name_str INTA Digital (INTA)
spelling Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilioEstravis Barcala, MaximilianoHerr, KatrinMarchelli, PaulaZiegenhagen, BirgitArana, María VeronicaBellora Pereyra, NicolásCalentamiento GlobalFisiología VegetalEstres TérmicoGenotiposFactores de TranscripciónSecuencia de ARNGlobal WarmingPlant PhysiologyHeat StressGenotypesTranscription FactorsRNA SecuenceGlobal warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underly ing the heat stress response is therefore crucial for the development of conservation strate gies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first tran scriptomic resource for the species. We also studied the changes in protein-coding tran scripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the exis tence of shared molecular features of heat stress responses across angiosperms, and iden tify genes of potential biotechnological application.EEA BarilocheFil: Estravis-Barcala, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Institute of Nuclear Technologies for Health; Argentina.Fil: Herr, Katrin. Philipps-Universität Marburg; Alemania.Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Ziegenhagen, Birgit. Philipps-Universität Marburg; AlemaniaFil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Arana, María Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Bellora Pereyra, Nicolás. Institute of Nuclear Technologies for Health (INTECNUS), National Scientific and Research Council (CONICET); Argentina.Public Library of Science (PLOS)2023-08-11T13:38:23Z2023-08-11T13:38:23Z2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14909https://journals.plos.org/plosone/article?id=10.1371/journal.pone.02466151932-6203https://doi.org/10.1371/journal.pone.0246615PLOS One 16 (3) : e0246615 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-30T11:24:22Zoai:localhost:20.500.12123/14909instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-30 11:24:22.481INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
spellingShingle Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
Estravis Barcala, Maximiliano
Calentamiento Global
Fisiología Vegetal
Estres Térmico
Genotipos
Factores de Transcripción
Secuencia de ARN
Global Warming
Plant Physiology
Heat Stress
Genotypes
Transcription Factors
RNA Secuence
title_short Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_full Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_fullStr Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_full_unstemmed Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_sort Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
dc.creator.none.fl_str_mv Estravis Barcala, Maximiliano
Herr, Katrin
Marchelli, Paula
Ziegenhagen, Birgit
Arana, María Veronica
Bellora Pereyra, Nicolás
author Estravis Barcala, Maximiliano
author_facet Estravis Barcala, Maximiliano
Herr, Katrin
Marchelli, Paula
Ziegenhagen, Birgit
Arana, María Veronica
Bellora Pereyra, Nicolás
author_role author
author2 Herr, Katrin
Marchelli, Paula
Ziegenhagen, Birgit
Arana, María Veronica
Bellora Pereyra, Nicolás
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Calentamiento Global
Fisiología Vegetal
Estres Térmico
Genotipos
Factores de Transcripción
Secuencia de ARN
Global Warming
Plant Physiology
Heat Stress
Genotypes
Transcription Factors
RNA Secuence
topic Calentamiento Global
Fisiología Vegetal
Estres Térmico
Genotipos
Factores de Transcripción
Secuencia de ARN
Global Warming
Plant Physiology
Heat Stress
Genotypes
Transcription Factors
RNA Secuence
dc.description.none.fl_txt_mv Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underly ing the heat stress response is therefore crucial for the development of conservation strate gies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first tran scriptomic resource for the species. We also studied the changes in protein-coding tran scripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the exis tence of shared molecular features of heat stress responses across angiosperms, and iden tify genes of potential biotechnological application.
EEA Bariloche
Fil: Estravis-Barcala, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Institute of Nuclear Technologies for Health; Argentina.
Fil: Herr, Katrin. Philipps-Universität Marburg; Alemania.
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ziegenhagen, Birgit. Philipps-Universität Marburg; Alemania
Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Arana, María Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Bellora Pereyra, Nicolás. Institute of Nuclear Technologies for Health (INTECNUS), National Scientific and Research Council (CONICET); Argentina.
description Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underly ing the heat stress response is therefore crucial for the development of conservation strate gies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first tran scriptomic resource for the species. We also studied the changes in protein-coding tran scripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the exis tence of shared molecular features of heat stress responses across angiosperms, and iden tify genes of potential biotechnological application.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
2023-08-11T13:38:23Z
2023-08-11T13:38:23Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14909
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246615
1932-6203
https://doi.org/10.1371/journal.pone.0246615
url http://hdl.handle.net/20.500.12123/14909
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246615
https://doi.org/10.1371/journal.pone.0246615
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLOS)
publisher.none.fl_str_mv Public Library of Science (PLOS)
dc.source.none.fl_str_mv PLOS One 16 (3) : e0246615 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.589754

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