Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio

Autores
Estravis Barcala, Maximiliano; Heer, Katrin; Marchelli, Paula; Ziegenhagen, Birgit; Arana, María Veronica; Bellora Pereyra, Nicolás
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first transcriptomic resource for the species. We also studied the changes in protein-coding transcripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the existence of shared molecular features of heat stress responses across angiosperms, and identify genes of potential biotechnological application.
Estación Experimental Agropecuaria Bariloche
Fil: Estravis Barcala, Maximiliano. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologías Nucleares para la Salud; Argentina
Fil: Heer, Katrin. Philipps-Universitat Marburg; Alemania
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ziegenhagen, Birgit. Philipps-Universität Marburg; Alemania
Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Arana, María Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Bellora Pereyra, Nicolás. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologías Nucleares para la Salud; Argentina
Fuente
Plos One 16 (3) : e0246615 (March 2021)
Materia
Nothofagus
Nothofagus pumilio
Bosque Primario
Bosque Templado
Calentamiento Global
Primary Forests
Temperate Forests
Global Warming
Región Patagónica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9730
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oai_identifier_str oai:localhost:20.500.12123/9730
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network_name_str INTA Digital (INTA)
spelling Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilioEstravis Barcala, MaximilianoHeer, KatrinMarchelli, PaulaZiegenhagen, BirgitArana, María VeronicaBellora Pereyra, NicolásNothofagusNothofagus pumilioBosque PrimarioBosque TempladoCalentamiento GlobalPrimary ForestsTemperate ForestsGlobal WarmingRegión PatagónicaGlobal warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first transcriptomic resource for the species. We also studied the changes in protein-coding transcripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the existence of shared molecular features of heat stress responses across angiosperms, and identify genes of potential biotechnological application.Estación Experimental Agropecuaria BarilocheFil: Estravis Barcala, Maximiliano. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologías Nucleares para la Salud; ArgentinaFil: Heer, Katrin. Philipps-Universitat Marburg; AlemaniaFil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Ziegenhagen, Birgit. Philipps-Universität Marburg; AlemaniaFil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Arana, María Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Bellora Pereyra, Nicolás. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologías Nucleares para la Salud; ArgentinaPlos One2021-07-05T12:53:47Z2021-07-05T12:53:47Z2021-03-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9730https://journals.plos.org/plosone/article?id=10.1371/journal.pone.02466151555-5887https://doi.org/10.1371/journal.pone.0246615Plos One 16 (3) : e0246615 (March 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:16Zoai:localhost:20.500.12123/9730instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:16.761INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
spellingShingle Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
Estravis Barcala, Maximiliano
Nothofagus
Nothofagus pumilio
Bosque Primario
Bosque Templado
Calentamiento Global
Primary Forests
Temperate Forests
Global Warming
Región Patagónica
title_short Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_full Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_fullStr Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_full_unstemmed Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
title_sort Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio
dc.creator.none.fl_str_mv Estravis Barcala, Maximiliano
Heer, Katrin
Marchelli, Paula
Ziegenhagen, Birgit
Arana, María Veronica
Bellora Pereyra, Nicolás
author Estravis Barcala, Maximiliano
author_facet Estravis Barcala, Maximiliano
Heer, Katrin
Marchelli, Paula
Ziegenhagen, Birgit
Arana, María Veronica
Bellora Pereyra, Nicolás
author_role author
author2 Heer, Katrin
Marchelli, Paula
Ziegenhagen, Birgit
Arana, María Veronica
Bellora Pereyra, Nicolás
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Nothofagus
Nothofagus pumilio
Bosque Primario
Bosque Templado
Calentamiento Global
Primary Forests
Temperate Forests
Global Warming
Región Patagónica
topic Nothofagus
Nothofagus pumilio
Bosque Primario
Bosque Templado
Calentamiento Global
Primary Forests
Temperate Forests
Global Warming
Región Patagónica
dc.description.none.fl_txt_mv Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first transcriptomic resource for the species. We also studied the changes in protein-coding transcripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the existence of shared molecular features of heat stress responses across angiosperms, and identify genes of potential biotechnological application.
Estación Experimental Agropecuaria Bariloche
Fil: Estravis Barcala, Maximiliano. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologías Nucleares para la Salud; Argentina
Fil: Heer, Katrin. Philipps-Universitat Marburg; Alemania
Fil: Marchelli, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Marchelli, Paula. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Ziegenhagen, Birgit. Philipps-Universität Marburg; Alemania
Fil: Arana, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Arana, María Veronica. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Bellora Pereyra, Nicolás. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologías Nucleares para la Salud; Argentina
description Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20˚C (control) or 34˚C (heat shock), we generated the first transcriptomic resource for the species. We also studied the changes in protein-coding transcripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the existence of shared molecular features of heat stress responses across angiosperms, and identify genes of potential biotechnological application.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-05T12:53:47Z
2021-07-05T12:53:47Z
2021-03-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9730
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246615
1555-5887
https://doi.org/10.1371/journal.pone.0246615
url http://hdl.handle.net/20.500.12123/9730
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0246615
https://doi.org/10.1371/journal.pone.0246615
identifier_str_mv 1555-5887
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv Plos One 16 (3) : e0246615 (March 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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