Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers

Autores
Reineri, Pablo Sebastian; Roldan Bernhard, Sergio Daniel; Principi, Santiago Alberto; Gerlero, Gustavo Daniel; Aller Atucha, Juan Florencio
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The objective of this study was to evaluate the effect of 7-day estradiol-progesterone-based [Treat(C)] and 5-day Co-Synch plus progesterone [Treat(Co-Sy)] protocols on ovulation time, pre-ovulatory follicle diameter, corpus luteum (CL) size and blood flow, progesterone (P4) concentration and pregnancy rate (PR) in beef heifers. In Experiment 1, a crossover design was applied (n = 9). For Treat(C), a progesterone intravaginal (PI) device was inserted, plus 2 mg of estradiol benzoate (day 0). On day 7, 500 µg of cloprostenol plus 0.5 mg of estradiol cypionate were administered, and PI was removed. For Treat(Co-Sy), on day 0, a PI was inserted plus 100 µg gonadotropin-releasing hormone (GnRH). On day 5, PI was removed, plus 500 µg of cloprostenol and 100 µg of GnRH were administered at 69–70 h later. From day one to ovulation day, dominant follicle was evaluated by ultrasonography. On days 4 and 8 post-ovulation, CL was evaluated by color Doppler, and P4 concentration was determined by chemiluminescence. In Experiment 2, a split-plot experimental design was used. Protocols followed were the same as in Experiment 1 [Treat(C); n = 310 and Treat(Co-Sy); n = 314]. Heifers were fixed-time artificially inseminated. Pregnancy was determined on day 41. In Experiment 1, the interval between PI removal and ovulation time was different between protocols (P < 0.01). In addition, P4 concentration was related to the CL size (P < 0.001), CL blood flow (P < 0.01) and protocols (P < 0.03). In Experiment 2, PR did not differ between protocols.
EEA Santiago del Estero
Fil: Reineri, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.
Fil: Reineri, Pablo Sebastián. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Roldan Bernhard, Sergio Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.
Fil: Principi, Santiago Alberto. Actividad privada; Argentina
Fil: Gerlero, Gustavo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Quimilí; Argentina
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fuente
Animal Reproduction Science 251 : 107223 (April 2023)
Materia
Ganado de Carne
Ovulación
Sincronización del Celo
Cuerpo Lúteo
Progesterona
Gestación
Beef Cattle
Ovulation
Oestrus Synchronization
Corpus Luteum
Progesterone
Pregnancy
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14331
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14331
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifersReineri, Pablo SebastianRoldan Bernhard, Sergio DanielPrincipi, Santiago AlbertoGerlero, Gustavo DanielAller Atucha, Juan FlorencioGanado de CarneOvulaciónSincronización del CeloCuerpo LúteoProgesteronaGestaciónBeef CattleOvulationOestrus SynchronizationCorpus LuteumProgesteronePregnancyThe objective of this study was to evaluate the effect of 7-day estradiol-progesterone-based [Treat(C)] and 5-day Co-Synch plus progesterone [Treat(Co-Sy)] protocols on ovulation time, pre-ovulatory follicle diameter, corpus luteum (CL) size and blood flow, progesterone (P4) concentration and pregnancy rate (PR) in beef heifers. In Experiment 1, a crossover design was applied (n = 9). For Treat(C), a progesterone intravaginal (PI) device was inserted, plus 2 mg of estradiol benzoate (day 0). On day 7, 500 µg of cloprostenol plus 0.5 mg of estradiol cypionate were administered, and PI was removed. For Treat(Co-Sy), on day 0, a PI was inserted plus 100 µg gonadotropin-releasing hormone (GnRH). On day 5, PI was removed, plus 500 µg of cloprostenol and 100 µg of GnRH were administered at 69–70 h later. From day one to ovulation day, dominant follicle was evaluated by ultrasonography. On days 4 and 8 post-ovulation, CL was evaluated by color Doppler, and P4 concentration was determined by chemiluminescence. In Experiment 2, a split-plot experimental design was used. Protocols followed were the same as in Experiment 1 [Treat(C); n = 310 and Treat(Co-Sy); n = 314]. Heifers were fixed-time artificially inseminated. Pregnancy was determined on day 41. In Experiment 1, the interval between PI removal and ovulation time was different between protocols (P < 0.01). In addition, P4 concentration was related to the CL size (P < 0.001), CL blood flow (P < 0.01) and protocols (P < 0.03). In Experiment 2, PR did not differ between protocols.EEA Santiago del EsteroFil: Reineri, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.Fil: Reineri, Pablo Sebastián. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Roldan Bernhard, Sergio Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.Fil: Principi, Santiago Alberto. Actividad privada; ArgentinaFil: Gerlero, Gustavo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Quimilí; ArgentinaFil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2024-03-272023-03-27T12:41:33Z2023-03-27T12:41:33Z2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14331https://www.sciencedirect.com/science/article/abs/pii/S03784320230003740378-4320https://doi.org/10.1016/j.anireprosci.2023.107223Animal Reproduction Science 251 : 107223 (April 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001/AR./Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnicoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:56Zoai:localhost:20.500.12123/14331instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:56.87INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
title Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
spellingShingle Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
Reineri, Pablo Sebastian
Ganado de Carne
Ovulación
Sincronización del Celo
Cuerpo Lúteo
Progesterona
Gestación
Beef Cattle
Ovulation
Oestrus Synchronization
Corpus Luteum
Progesterone
Pregnancy
title_short Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
title_full Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
title_fullStr Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
title_full_unstemmed Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
title_sort Effects of two protocols of ovulation synchronization on corpus luteum size and blood flow, progesterone concentration, and pregnancy rate in beef heifers
dc.creator.none.fl_str_mv Reineri, Pablo Sebastian
Roldan Bernhard, Sergio Daniel
Principi, Santiago Alberto
Gerlero, Gustavo Daniel
Aller Atucha, Juan Florencio
author Reineri, Pablo Sebastian
author_facet Reineri, Pablo Sebastian
Roldan Bernhard, Sergio Daniel
Principi, Santiago Alberto
Gerlero, Gustavo Daniel
Aller Atucha, Juan Florencio
author_role author
author2 Roldan Bernhard, Sergio Daniel
Principi, Santiago Alberto
Gerlero, Gustavo Daniel
Aller Atucha, Juan Florencio
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ganado de Carne
Ovulación
Sincronización del Celo
Cuerpo Lúteo
Progesterona
Gestación
Beef Cattle
Ovulation
Oestrus Synchronization
Corpus Luteum
Progesterone
Pregnancy
topic Ganado de Carne
Ovulación
Sincronización del Celo
Cuerpo Lúteo
Progesterona
Gestación
Beef Cattle
Ovulation
Oestrus Synchronization
Corpus Luteum
Progesterone
Pregnancy
dc.description.none.fl_txt_mv The objective of this study was to evaluate the effect of 7-day estradiol-progesterone-based [Treat(C)] and 5-day Co-Synch plus progesterone [Treat(Co-Sy)] protocols on ovulation time, pre-ovulatory follicle diameter, corpus luteum (CL) size and blood flow, progesterone (P4) concentration and pregnancy rate (PR) in beef heifers. In Experiment 1, a crossover design was applied (n = 9). For Treat(C), a progesterone intravaginal (PI) device was inserted, plus 2 mg of estradiol benzoate (day 0). On day 7, 500 µg of cloprostenol plus 0.5 mg of estradiol cypionate were administered, and PI was removed. For Treat(Co-Sy), on day 0, a PI was inserted plus 100 µg gonadotropin-releasing hormone (GnRH). On day 5, PI was removed, plus 500 µg of cloprostenol and 100 µg of GnRH were administered at 69–70 h later. From day one to ovulation day, dominant follicle was evaluated by ultrasonography. On days 4 and 8 post-ovulation, CL was evaluated by color Doppler, and P4 concentration was determined by chemiluminescence. In Experiment 2, a split-plot experimental design was used. Protocols followed were the same as in Experiment 1 [Treat(C); n = 310 and Treat(Co-Sy); n = 314]. Heifers were fixed-time artificially inseminated. Pregnancy was determined on day 41. In Experiment 1, the interval between PI removal and ovulation time was different between protocols (P < 0.01). In addition, P4 concentration was related to the CL size (P < 0.001), CL blood flow (P < 0.01) and protocols (P < 0.03). In Experiment 2, PR did not differ between protocols.
EEA Santiago del Estero
Fil: Reineri, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.
Fil: Reineri, Pablo Sebastián. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Roldan Bernhard, Sergio Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.
Fil: Principi, Santiago Alberto. Actividad privada; Argentina
Fil: Gerlero, Gustavo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Quimilí; Argentina
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
description The objective of this study was to evaluate the effect of 7-day estradiol-progesterone-based [Treat(C)] and 5-day Co-Synch plus progesterone [Treat(Co-Sy)] protocols on ovulation time, pre-ovulatory follicle diameter, corpus luteum (CL) size and blood flow, progesterone (P4) concentration and pregnancy rate (PR) in beef heifers. In Experiment 1, a crossover design was applied (n = 9). For Treat(C), a progesterone intravaginal (PI) device was inserted, plus 2 mg of estradiol benzoate (day 0). On day 7, 500 µg of cloprostenol plus 0.5 mg of estradiol cypionate were administered, and PI was removed. For Treat(Co-Sy), on day 0, a PI was inserted plus 100 µg gonadotropin-releasing hormone (GnRH). On day 5, PI was removed, plus 500 µg of cloprostenol and 100 µg of GnRH were administered at 69–70 h later. From day one to ovulation day, dominant follicle was evaluated by ultrasonography. On days 4 and 8 post-ovulation, CL was evaluated by color Doppler, and P4 concentration was determined by chemiluminescence. In Experiment 2, a split-plot experimental design was used. Protocols followed were the same as in Experiment 1 [Treat(C); n = 310 and Treat(Co-Sy); n = 314]. Heifers were fixed-time artificially inseminated. Pregnancy was determined on day 41. In Experiment 1, the interval between PI removal and ovulation time was different between protocols (P < 0.01). In addition, P4 concentration was related to the CL size (P < 0.001), CL blood flow (P < 0.01) and protocols (P < 0.03). In Experiment 2, PR did not differ between protocols.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-27T12:41:33Z
2023-03-27T12:41:33Z
2023-04
info:eu-repo/date/embargoEnd/2024-03-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14331
https://www.sciencedirect.com/science/article/abs/pii/S0378432023000374
0378-4320
https://doi.org/10.1016/j.anireprosci.2023.107223
url http://hdl.handle.net/20.500.12123/14331
https://www.sciencedirect.com/science/article/abs/pii/S0378432023000374
https://doi.org/10.1016/j.anireprosci.2023.107223
identifier_str_mv 0378-4320
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001/AR./Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnico
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Animal Reproduction Science 251 : 107223 (April 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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