A link between Notch and progesterone maintains the functionality of the rat corpus luteum

Autores
Accialini, Paula Lucia; Hernandez, Silvia Fátima; Bas, Diana Ester; Pazos Maidana, María Camila; Irusta, Griselda; Abramovich, Dalhia Nurit; Tesone, Marta
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we investigated the interaction between the Notch pathway and progesterone to maintain the functionality of the corpus luteum(CL).WhenNotch signaling is activated, the g-secretase complex releases the active intracellular domains (NICD) of their receptors, which exert survival effects. We designed studies to analyze whether the in vitro inhibition of Notch affects progesterone production, steroidogenic regulators, apoptotic parameters, and signaling transduction pathways in the cultures of CL isolated from pregnant and superovulated rats. We detected a decrease in progesterone production when corpora lutea (CL) were incubated with N-(N-(3,5- difluorophenacetyl-L-alanyl))-S-phenylglycine t-butyl ester (DAPT), a g-secretase inhibitor. This effect could be in part due to the decrease detected in the CL protein levels of P450scc because STAR and 3b-hydroxysteroid dehydrogenase were not affected by Notch inhibition. Besides, the addition of aminoglutethimide to the CL culture medium decreased NICD of NOTCH1. We observed an increase in the expression of active CASPASE3 (CASP3) after inhibition by Notch,whichwas reversed by the presence of progesterone. The BAX:BCLXL ratio was increased in CL treated with DAPT and the presence of progesterone reversed this effect. In addition, phosphorylation of AKTwas inhibited inCL treated withDAPT, but had no effect on ERK activation. To demonstrate that the action ofDAPTis specifically related with the inhibition of Notch, CLswere incubated with DLL4 antibody and a decrease in progesterone production was detected. These results suggest the existence of a novel link between progesterone and the Notch signaling pathway to maintain the functionality of the CL.
Fil: Accialini, Paula Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Hernandez, Silvia Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Bas, Diana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Pazos Maidana, María Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
Notch
Ovary
Corpus Luteum
Progesterone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3061
Acceder
id CONICETDig_2aeca379f0be3d7653ce98267ef658b1
oai_identifier_str oai:ri.conicet.gov.ar:11336/3061
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A link between Notch and progesterone maintains the functionality of the rat corpus luteumAccialini, Paula LuciaHernandez, Silvia FátimaBas, Diana EsterPazos Maidana, María CamilaIrusta, GriseldaAbramovich, Dalhia NuritTesone, MartaNotchOvaryCorpus LuteumProgesteronehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this study, we investigated the interaction between the Notch pathway and progesterone to maintain the functionality of the corpus luteum(CL).WhenNotch signaling is activated, the g-secretase complex releases the active intracellular domains (NICD) of their receptors, which exert survival effects. We designed studies to analyze whether the in vitro inhibition of Notch affects progesterone production, steroidogenic regulators, apoptotic parameters, and signaling transduction pathways in the cultures of CL isolated from pregnant and superovulated rats. We detected a decrease in progesterone production when corpora lutea (CL) were incubated with N-(N-(3,5- difluorophenacetyl-L-alanyl))-S-phenylglycine t-butyl ester (DAPT), a g-secretase inhibitor. This effect could be in part due to the decrease detected in the CL protein levels of P450scc because STAR and 3b-hydroxysteroid dehydrogenase were not affected by Notch inhibition. Besides, the addition of aminoglutethimide to the CL culture medium decreased NICD of NOTCH1. We observed an increase in the expression of active CASPASE3 (CASP3) after inhibition by Notch,whichwas reversed by the presence of progesterone. The BAX:BCLXL ratio was increased in CL treated with DAPT and the presence of progesterone reversed this effect. In addition, phosphorylation of AKTwas inhibited inCL treated withDAPT, but had no effect on ERK activation. To demonstrate that the action ofDAPTis specifically related with the inhibition of Notch, CLswere incubated with DLL4 antibody and a decrease in progesterone production was detected. These results suggest the existence of a novel link between progesterone and the Notch signaling pathway to maintain the functionality of the CL.Fil: Accialini, Paula Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Hernandez, Silvia Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Bas, Diana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Pazos Maidana, María Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaBioscientifica2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3061Accialini, Paula Lucia; Hernandez, Silvia Fátima; Bas, Diana Ester; Pazos Maidana, María Camila; Irusta, Griselda; et al.; A link between Notch and progesterone maintains the functionality of the rat corpus luteum; Bioscientifica; Reproduction; 149; 1; 1-2015; 1-101470-16261741-7899enginfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/149/1/1.abstractinfo:eu-repo/semantics/altIdentifier/doi/doi:10.1530/REP-14-0449info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:09Zoai:ri.conicet.gov.ar:11336/3061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:09.331CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A link between Notch and progesterone maintains the functionality of the rat corpus luteum
title A link between Notch and progesterone maintains the functionality of the rat corpus luteum
spellingShingle A link between Notch and progesterone maintains the functionality of the rat corpus luteum
Accialini, Paula Lucia
Notch
Ovary
Corpus Luteum
Progesterone
title_short A link between Notch and progesterone maintains the functionality of the rat corpus luteum
title_full A link between Notch and progesterone maintains the functionality of the rat corpus luteum
title_fullStr A link between Notch and progesterone maintains the functionality of the rat corpus luteum
title_full_unstemmed A link between Notch and progesterone maintains the functionality of the rat corpus luteum
title_sort A link between Notch and progesterone maintains the functionality of the rat corpus luteum
dc.creator.none.fl_str_mv Accialini, Paula Lucia
Hernandez, Silvia Fátima
Bas, Diana Ester
Pazos Maidana, María Camila
Irusta, Griselda
Abramovich, Dalhia Nurit
Tesone, Marta
author Accialini, Paula Lucia
author_facet Accialini, Paula Lucia
Hernandez, Silvia Fátima
Bas, Diana Ester
Pazos Maidana, María Camila
Irusta, Griselda
Abramovich, Dalhia Nurit
Tesone, Marta
author_role author
author2 Hernandez, Silvia Fátima
Bas, Diana Ester
Pazos Maidana, María Camila
Irusta, Griselda
Abramovich, Dalhia Nurit
Tesone, Marta
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Notch
Ovary
Corpus Luteum
Progesterone
topic Notch
Ovary
Corpus Luteum
Progesterone
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, we investigated the interaction between the Notch pathway and progesterone to maintain the functionality of the corpus luteum(CL).WhenNotch signaling is activated, the g-secretase complex releases the active intracellular domains (NICD) of their receptors, which exert survival effects. We designed studies to analyze whether the in vitro inhibition of Notch affects progesterone production, steroidogenic regulators, apoptotic parameters, and signaling transduction pathways in the cultures of CL isolated from pregnant and superovulated rats. We detected a decrease in progesterone production when corpora lutea (CL) were incubated with N-(N-(3,5- difluorophenacetyl-L-alanyl))-S-phenylglycine t-butyl ester (DAPT), a g-secretase inhibitor. This effect could be in part due to the decrease detected in the CL protein levels of P450scc because STAR and 3b-hydroxysteroid dehydrogenase were not affected by Notch inhibition. Besides, the addition of aminoglutethimide to the CL culture medium decreased NICD of NOTCH1. We observed an increase in the expression of active CASPASE3 (CASP3) after inhibition by Notch,whichwas reversed by the presence of progesterone. The BAX:BCLXL ratio was increased in CL treated with DAPT and the presence of progesterone reversed this effect. In addition, phosphorylation of AKTwas inhibited inCL treated withDAPT, but had no effect on ERK activation. To demonstrate that the action ofDAPTis specifically related with the inhibition of Notch, CLswere incubated with DLL4 antibody and a decrease in progesterone production was detected. These results suggest the existence of a novel link between progesterone and the Notch signaling pathway to maintain the functionality of the CL.
Fil: Accialini, Paula Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Hernandez, Silvia Fátima. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Bas, Diana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Pazos Maidana, María Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Abramovich, Dalhia Nurit. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description In this study, we investigated the interaction between the Notch pathway and progesterone to maintain the functionality of the corpus luteum(CL).WhenNotch signaling is activated, the g-secretase complex releases the active intracellular domains (NICD) of their receptors, which exert survival effects. We designed studies to analyze whether the in vitro inhibition of Notch affects progesterone production, steroidogenic regulators, apoptotic parameters, and signaling transduction pathways in the cultures of CL isolated from pregnant and superovulated rats. We detected a decrease in progesterone production when corpora lutea (CL) were incubated with N-(N-(3,5- difluorophenacetyl-L-alanyl))-S-phenylglycine t-butyl ester (DAPT), a g-secretase inhibitor. This effect could be in part due to the decrease detected in the CL protein levels of P450scc because STAR and 3b-hydroxysteroid dehydrogenase were not affected by Notch inhibition. Besides, the addition of aminoglutethimide to the CL culture medium decreased NICD of NOTCH1. We observed an increase in the expression of active CASPASE3 (CASP3) after inhibition by Notch,whichwas reversed by the presence of progesterone. The BAX:BCLXL ratio was increased in CL treated with DAPT and the presence of progesterone reversed this effect. In addition, phosphorylation of AKTwas inhibited inCL treated withDAPT, but had no effect on ERK activation. To demonstrate that the action ofDAPTis specifically related with the inhibition of Notch, CLswere incubated with DLL4 antibody and a decrease in progesterone production was detected. These results suggest the existence of a novel link between progesterone and the Notch signaling pathway to maintain the functionality of the CL.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3061
Accialini, Paula Lucia; Hernandez, Silvia Fátima; Bas, Diana Ester; Pazos Maidana, María Camila; Irusta, Griselda; et al.; A link between Notch and progesterone maintains the functionality of the rat corpus luteum; Bioscientifica; Reproduction; 149; 1; 1-2015; 1-10
1470-1626
1741-7899
url http://hdl.handle.net/11336/3061
identifier_str_mv Accialini, Paula Lucia; Hernandez, Silvia Fátima; Bas, Diana Ester; Pazos Maidana, María Camila; Irusta, Griselda; et al.; A link between Notch and progesterone maintains the functionality of the rat corpus luteum; Bioscientifica; Reproduction; 149; 1; 1-2015; 1-10
1470-1626
1741-7899
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/149/1/1.abstract
info:eu-repo/semantics/altIdentifier/doi/doi:10.1530/REP-14-0449
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bioscientifica
publisher.none.fl_str_mv Bioscientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269784752783360
score 13.13397

Publicaciones similares