Estradiol-17β Injection Induces Ovulation in Llamas

Autores
Bianchi, Carolina Paula; Benavente, Micaela Andrea; Viviani, Florencia; Gallelli, Maria Florencia; Aba, Marcelo Alfredo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study aimed to investigate the effect of three different doses of estradiol-17b onovulation and subsequent luteal development and function in llamas. Twenty-three llamaswere examined daily by transrectal ultrasonography until the detection of an ovulatoryfollicle (≥8mm). Thereafter, animals were divided into five groups: Control (n = 3; treatedwith 1.6ml of saline solution), GnRH group (n = 6, treated with an intravenous injectionof 8.4 μg Buserelin), and estradiol groups that received 0.6mg (E1, n = 4), 1mg (E2,n = 4), or 1.6mg (E3, n = 6) of estradiol-17b intravenously. Detection of ovulationwas based on ultrasonographic visualization of disappearance of the largest follicleand subsequent presence of a newly formed corpus luteum (CL) and progesteroneconcentration exceeding 1 ng ml−1. Daily blood samples were collected to determineplasma progesterone concentration. Ovulation rate was 0% for control and E1 groups,25% for E2 group, and 100% for GnRH and E3 groups. Differences in the meanCL diameter between GnRH and E3 groups were not statistically significant. Plasmaprogesterone concentration was similar between groups during the different days inovulated animals. However, the day that the plasma progesterone concentration wasabove 1 ng ml−1 and the day that the highest plasma progesterone concentration wasachieved differed among E3 and GnRH groups, occurring later in females treated withestradiol. In conclusion, an injection of estradiol-17b is capable of inducing ovulation inllamas and the response depends on the dose used. Most of the animals required thehighest tested dose (1.6mg) to induce the ovulatory process. Although the CL diameterin females induced to ovulate with estradiol was similar to that in llamas induced toovulate with a GnRH analog, the rise in plasma progesterone concentration above 1 ngml−1 and the peak progesterone concentration were attained 1 day later in the estradioltreated females.
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Viviani, Florencia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gallelli, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Materia
CORPUS LUTEUM
ESTRADIOL-17Β
LLAMAS
OVULATION
PROGESTERONE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121367
Acceder
id CONICETDig_fa0e3b36652a607b65423078b835713e
oai_identifier_str oai:ri.conicet.gov.ar:11336/121367
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estradiol-17β Injection Induces Ovulation in LlamasBianchi, Carolina PaulaBenavente, Micaela AndreaViviani, FlorenciaGallelli, Maria FlorenciaAba, Marcelo AlfredoCORPUS LUTEUMESTRADIOL-17ΒLLAMASOVULATIONPROGESTERONEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4This study aimed to investigate the effect of three different doses of estradiol-17b onovulation and subsequent luteal development and function in llamas. Twenty-three llamaswere examined daily by transrectal ultrasonography until the detection of an ovulatoryfollicle (≥8mm). Thereafter, animals were divided into five groups: Control (n = 3; treatedwith 1.6ml of saline solution), GnRH group (n = 6, treated with an intravenous injectionof 8.4 μg Buserelin), and estradiol groups that received 0.6mg (E1, n = 4), 1mg (E2,n = 4), or 1.6mg (E3, n = 6) of estradiol-17b intravenously. Detection of ovulationwas based on ultrasonographic visualization of disappearance of the largest follicleand subsequent presence of a newly formed corpus luteum (CL) and progesteroneconcentration exceeding 1 ng ml−1. Daily blood samples were collected to determineplasma progesterone concentration. Ovulation rate was 0% for control and E1 groups,25% for E2 group, and 100% for GnRH and E3 groups. Differences in the meanCL diameter between GnRH and E3 groups were not statistically significant. Plasmaprogesterone concentration was similar between groups during the different days inovulated animals. However, the day that the plasma progesterone concentration wasabove 1 ng ml−1 and the day that the highest plasma progesterone concentration wasachieved differed among E3 and GnRH groups, occurring later in females treated withestradiol. In conclusion, an injection of estradiol-17b is capable of inducing ovulation inllamas and the response depends on the dose used. Most of the animals required thehighest tested dose (1.6mg) to induce the ovulatory process. Although the CL diameterin females induced to ovulate with estradiol was similar to that in llamas induced toovulate with a GnRH analog, the rise in plasma progesterone concentration above 1 ngml−1 and the peak progesterone concentration were attained 1 day later in the estradioltreated females.Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Viviani, Florencia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Gallelli, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFrontiers Media S.A.2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121367Bianchi, Carolina Paula; Benavente, Micaela Andrea; Viviani, Florencia; Gallelli, Maria Florencia; Aba, Marcelo Alfredo; Estradiol-17β Injection Induces Ovulation in Llamas; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 10-2020; 1-6229717692297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fvets.2020.576204/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.576204info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:26Zoai:ri.conicet.gov.ar:11336/121367instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:26.32CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estradiol-17β Injection Induces Ovulation in Llamas
title Estradiol-17β Injection Induces Ovulation in Llamas
spellingShingle Estradiol-17β Injection Induces Ovulation in Llamas
Bianchi, Carolina Paula
CORPUS LUTEUM
ESTRADIOL-17Β
LLAMAS
OVULATION
PROGESTERONE
title_short Estradiol-17β Injection Induces Ovulation in Llamas
title_full Estradiol-17β Injection Induces Ovulation in Llamas
title_fullStr Estradiol-17β Injection Induces Ovulation in Llamas
title_full_unstemmed Estradiol-17β Injection Induces Ovulation in Llamas
title_sort Estradiol-17β Injection Induces Ovulation in Llamas
dc.creator.none.fl_str_mv Bianchi, Carolina Paula
Benavente, Micaela Andrea
Viviani, Florencia
Gallelli, Maria Florencia
Aba, Marcelo Alfredo
author Bianchi, Carolina Paula
author_facet Bianchi, Carolina Paula
Benavente, Micaela Andrea
Viviani, Florencia
Gallelli, Maria Florencia
Aba, Marcelo Alfredo
author_role author
author2 Benavente, Micaela Andrea
Viviani, Florencia
Gallelli, Maria Florencia
Aba, Marcelo Alfredo
author2_role author
author
author
author
dc.subject.none.fl_str_mv CORPUS LUTEUM
ESTRADIOL-17Β
LLAMAS
OVULATION
PROGESTERONE
topic CORPUS LUTEUM
ESTRADIOL-17Β
LLAMAS
OVULATION
PROGESTERONE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv This study aimed to investigate the effect of three different doses of estradiol-17b onovulation and subsequent luteal development and function in llamas. Twenty-three llamaswere examined daily by transrectal ultrasonography until the detection of an ovulatoryfollicle (≥8mm). Thereafter, animals were divided into five groups: Control (n = 3; treatedwith 1.6ml of saline solution), GnRH group (n = 6, treated with an intravenous injectionof 8.4 μg Buserelin), and estradiol groups that received 0.6mg (E1, n = 4), 1mg (E2,n = 4), or 1.6mg (E3, n = 6) of estradiol-17b intravenously. Detection of ovulationwas based on ultrasonographic visualization of disappearance of the largest follicleand subsequent presence of a newly formed corpus luteum (CL) and progesteroneconcentration exceeding 1 ng ml−1. Daily blood samples were collected to determineplasma progesterone concentration. Ovulation rate was 0% for control and E1 groups,25% for E2 group, and 100% for GnRH and E3 groups. Differences in the meanCL diameter between GnRH and E3 groups were not statistically significant. Plasmaprogesterone concentration was similar between groups during the different days inovulated animals. However, the day that the plasma progesterone concentration wasabove 1 ng ml−1 and the day that the highest plasma progesterone concentration wasachieved differed among E3 and GnRH groups, occurring later in females treated withestradiol. In conclusion, an injection of estradiol-17b is capable of inducing ovulation inllamas and the response depends on the dose used. Most of the animals required thehighest tested dose (1.6mg) to induce the ovulatory process. Although the CL diameterin females induced to ovulate with estradiol was similar to that in llamas induced toovulate with a GnRH analog, the rise in plasma progesterone concentration above 1 ngml−1 and the peak progesterone concentration were attained 1 day later in the estradioltreated females.
Fil: Bianchi, Carolina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Benavente, Micaela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Viviani, Florencia. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gallelli, Maria Florencia. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aba, Marcelo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
description This study aimed to investigate the effect of three different doses of estradiol-17b onovulation and subsequent luteal development and function in llamas. Twenty-three llamaswere examined daily by transrectal ultrasonography until the detection of an ovulatoryfollicle (≥8mm). Thereafter, animals were divided into five groups: Control (n = 3; treatedwith 1.6ml of saline solution), GnRH group (n = 6, treated with an intravenous injectionof 8.4 μg Buserelin), and estradiol groups that received 0.6mg (E1, n = 4), 1mg (E2,n = 4), or 1.6mg (E3, n = 6) of estradiol-17b intravenously. Detection of ovulationwas based on ultrasonographic visualization of disappearance of the largest follicleand subsequent presence of a newly formed corpus luteum (CL) and progesteroneconcentration exceeding 1 ng ml−1. Daily blood samples were collected to determineplasma progesterone concentration. Ovulation rate was 0% for control and E1 groups,25% for E2 group, and 100% for GnRH and E3 groups. Differences in the meanCL diameter between GnRH and E3 groups were not statistically significant. Plasmaprogesterone concentration was similar between groups during the different days inovulated animals. However, the day that the plasma progesterone concentration wasabove 1 ng ml−1 and the day that the highest plasma progesterone concentration wasachieved differed among E3 and GnRH groups, occurring later in females treated withestradiol. In conclusion, an injection of estradiol-17b is capable of inducing ovulation inllamas and the response depends on the dose used. Most of the animals required thehighest tested dose (1.6mg) to induce the ovulatory process. Although the CL diameterin females induced to ovulate with estradiol was similar to that in llamas induced toovulate with a GnRH analog, the rise in plasma progesterone concentration above 1 ngml−1 and the peak progesterone concentration were attained 1 day later in the estradioltreated females.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121367
Bianchi, Carolina Paula; Benavente, Micaela Andrea; Viviani, Florencia; Gallelli, Maria Florencia; Aba, Marcelo Alfredo; Estradiol-17β Injection Induces Ovulation in Llamas; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 10-2020; 1-6
22971769
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121367
identifier_str_mv Bianchi, Carolina Paula; Benavente, Micaela Andrea; Viviani, Florencia; Gallelli, Maria Florencia; Aba, Marcelo Alfredo; Estradiol-17β Injection Induces Ovulation in Llamas; Frontiers Media S.A.; Frontiers in Veterinary Science; 7; 10-2020; 1-6
22971769
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fvets.2020.576204/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2020.576204
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614394876526592
score 13.070432

Publicaciones similares