Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle

Autores
Primo, María Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni, Susana Marta; Echaide, Ignacio Eduardo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.
EEA Rafaela
Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Torioni, Susana Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Echaide, Ignacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fuente
Ticks and Tick-borne Diseases 12 (5) : 101787 (September 2021)
Materia
Anaplasma
Anaplasma marginale
Ganado Bovino
Oxitetraciclina
Genotipos
Cattle
Oxytetracycline
Genotypes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9851
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9851
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattlePrimo, María EvangelinaSarli, MacarenaThompson, Carolina SoledadTorioni, Susana MartaEchaide, Ignacio EduardoAnaplasmaAnaplasma marginaleGanado BovinoOxitetraciclinaGenotiposCattleOxytetracyclineGenotypesThe aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.EEA RafaelaFil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Torioni, Susana Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.Fil: Echaide, Ignacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.Elsevierinfo:eu-repo/date/embargoEnd/2022-07-202021-07-20T18:19:36Z2021-07-20T18:19:36Z2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9851https://www.sciencedirect.com/science/article/abs/pii/S1877959X210014001877-959Xhttps://doi.org/10.1016/j.ttbdis.2021.101787Ticks and Tick-borne Diseases 12 (5) : 101787 (September 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-11T10:23:49Zoai:localhost:20.500.12123/9851instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:23:50.144INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
spellingShingle Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
Primo, María Evangelina
Anaplasma
Anaplasma marginale
Ganado Bovino
Oxitetraciclina
Genotipos
Cattle
Oxytetracycline
Genotypes
title_short Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_full Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_fullStr Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_full_unstemmed Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_sort Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
dc.creator.none.fl_str_mv Primo, María Evangelina
Sarli, Macarena
Thompson, Carolina Soledad
Torioni, Susana Marta
Echaide, Ignacio Eduardo
author Primo, María Evangelina
author_facet Primo, María Evangelina
Sarli, Macarena
Thompson, Carolina Soledad
Torioni, Susana Marta
Echaide, Ignacio Eduardo
author_role author
author2 Sarli, Macarena
Thompson, Carolina Soledad
Torioni, Susana Marta
Echaide, Ignacio Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Anaplasma
Anaplasma marginale
Ganado Bovino
Oxitetraciclina
Genotipos
Cattle
Oxytetracycline
Genotypes
topic Anaplasma
Anaplasma marginale
Ganado Bovino
Oxitetraciclina
Genotipos
Cattle
Oxytetracycline
Genotypes
dc.description.none.fl_txt_mv The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.
EEA Rafaela
Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Primo, María Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Torioni, Susana Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Echaide, Ignacio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina.
Fil: Echaide, Ignacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina.
description The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-20T18:19:36Z
2021-07-20T18:19:36Z
2021-09
info:eu-repo/date/embargoEnd/2022-07-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9851
https://www.sciencedirect.com/science/article/abs/pii/S1877959X21001400
1877-959X
https://doi.org/10.1016/j.ttbdis.2021.101787
url http://hdl.handle.net/20.500.12123/9851
https://www.sciencedirect.com/science/article/abs/pii/S1877959X21001400
https://doi.org/10.1016/j.ttbdis.2021.101787
identifier_str_mv 1877-959X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ticks and Tick-borne Diseases 12 (5) : 101787 (September 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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