Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle

Autores
Primo, Maria Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni de Echaide, Susana Marta; Echaide, Ignacio Eduardo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.
Fil: Primo, Maria Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Torioni de Echaide, Susana Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Echaide, Ignacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Materia
ANAPLASMA MARGINALE
CATTLE
GENOTYPE
MSP1Α
OXYTETRACYCLINE
STRAIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/167352
Acceder
id CONICETDig_8104cb77acfcb4e045ff4c444bd72ddc
oai_identifier_str oai:ri.conicet.gov.ar:11336/167352
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattlePrimo, Maria EvangelinaSarli, MacarenaThompson, Carolina SoledadTorioni de Echaide, Susana MartaEchaide, Ignacio EduardoANAPLASMA MARGINALECATTLEGENOTYPEMSP1ΑOXYTETRACYCLINESTRAINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.Fil: Primo, Maria Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Torioni de Echaide, Susana Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Echaide, Ignacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaElsevier Gmbh2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167352Primo, Maria Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni de Echaide, Susana Marta; Echaide, Ignacio Eduardo; Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle; Elsevier Gmbh; Ticks and Tick-borne Diseases; 12; 5; 9-2021; 1-211877-959XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1877959X21001400info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ttbdis.2021.101787info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:29Zoai:ri.conicet.gov.ar:11336/167352instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:29.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
spellingShingle Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
Primo, Maria Evangelina
ANAPLASMA MARGINALE
CATTLE
GENOTYPE
MSP1Α
OXYTETRACYCLINE
STRAIN
title_short Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_full Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_fullStr Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_full_unstemmed Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
title_sort Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle
dc.creator.none.fl_str_mv Primo, Maria Evangelina
Sarli, Macarena
Thompson, Carolina Soledad
Torioni de Echaide, Susana Marta
Echaide, Ignacio Eduardo
author Primo, Maria Evangelina
author_facet Primo, Maria Evangelina
Sarli, Macarena
Thompson, Carolina Soledad
Torioni de Echaide, Susana Marta
Echaide, Ignacio Eduardo
author_role author
author2 Sarli, Macarena
Thompson, Carolina Soledad
Torioni de Echaide, Susana Marta
Echaide, Ignacio Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANAPLASMA MARGINALE
CATTLE
GENOTYPE
MSP1Α
OXYTETRACYCLINE
STRAIN
topic ANAPLASMA MARGINALE
CATTLE
GENOTYPE
MSP1Α
OXYTETRACYCLINE
STRAIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.
Fil: Primo, Maria Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Sarli, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Thompson, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Torioni de Echaide, Susana Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Echaide, Ignacio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
description The aim of this study was to evaluate the influence of the long-acting oxytetracycline (OTC) treatment on A. marginale genotypes of the isolate S1P, by analyzing the msp1α genotype based on a microsatellite (ms) and tandem repeat sequences (TRS) located at the 5´ end of the gene. DNA samples were obtained from a longitudinal study of chemosterilization; 10 2-year-old steers were experimentally infected with blood from a splenectomized calf inoculated with the A. marginale isolate S1P. All the steers had received a first dose of 20 mg kg−1 OTC to treat acute disease, and once recovered all steers received a sterilizing treatment based on three doses of 20 mg kg−1 OTC 7 days apart. Blood from two steers not sterilized by the treatment was inoculated into two splenectomized calves (receptors) 104 days after treatment. DNA samples (S) used for msp1α amplification were obtained from i) the donor calf (S0), ii) 10 steers during acute disease (S1), after the first antibiotic treatment (S2), and after the chemosterilization procedure (S3 and S4), and iii) two receptor calves (S5). Thirty clones from the donor calf and at least 5 clones from the other DNA samples were analyzed. The genotype E/αββββГ msp1α identified in the donor calf and steers, before OTC treatment, was not detected either in steers that continued infected after the sterilizing treatment or in the receptor calves, in which only genotype C/EϕFF msp1α was observed. These results highlight the existence of A. marginale genotypes with different sensitivity to OTC and the importance of other variables to successfully sterilize the carriers.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/167352
Primo, Maria Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni de Echaide, Susana Marta; Echaide, Ignacio Eduardo; Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle; Elsevier Gmbh; Ticks and Tick-borne Diseases; 12; 5; 9-2021; 1-21
1877-959X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/167352
identifier_str_mv Primo, Maria Evangelina; Sarli, Macarena; Thompson, Carolina Soledad; Torioni de Echaide, Susana Marta; Echaide, Ignacio Eduardo; Sensitivity of Anaplasma marginale genotypes to oxytetracycline assessed by analyzing the msp1α gene in experimentally infected cattle; Elsevier Gmbh; Ticks and Tick-borne Diseases; 12; 5; 9-2021; 1-21
1877-959X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1877959X21001400
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ttbdis.2021.101787
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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