Identification and characterization of a new potyvirus infecting cucurbits

Autores
Perotto, Maria Cecilia; Pozzi, Elizabeth Alicia; Celli, Marcos Giovani; Luciani, Cecilia Elizabeth; Mitidieri, Mariel Silvina; Conci, Vilma Cecilia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
Instituto de Patología Vegetal
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Luciani, Cecilia E. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Archives of virology 163 (3) : 719–724. (March 2018)
Materia
Potyviruses
Identification
Plant Viruses
Squashes
Potyvirus
Cucurbitaceae
Identificación
Virus de las Plantas
Calabazas
San Pedro, Buenos Aires
Illumina Sequencing
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3632
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3632
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network_name_str INTA Digital (INTA)
spelling Identification and characterization of a new potyvirus infecting cucurbitsPerotto, Maria CeciliaPozzi, Elizabeth AliciaCelli, Marcos GiovaniLuciani, Cecilia ElizabethMitidieri, Mariel SilvinaConci, Vilma CeciliaPotyvirusesIdentificationPlant VirusesSquashesPotyvirusCucurbitaceaeIdentificaciónVirus de las PlantasCalabazasSan Pedro, Buenos AiresIllumina SequencingA new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.Instituto de Patología VegetalFil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; ArgentinaFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; ArgentinaFil: Luciani, Cecilia E. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; ArgentinaFil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2018-10-18T15:21:25Z2018-10-18T15:21:25Z2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3632https://link.springer.com/article/10.1007%2Fs00705-017-3660-2#citeas0304-86081432-8798 (Online)https://doi.org/10.1007/s00705-017-3660-2Archives of virology 163 (3) : 719–724. (March 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengSan Pedro (inhabited place)Buenos Aires (province)info:eu-repo/semantics/restrictedAccess2025-10-16T09:29:20Zoai:localhost:20.500.12123/3632instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:20.737INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification and characterization of a new potyvirus infecting cucurbits
title Identification and characterization of a new potyvirus infecting cucurbits
spellingShingle Identification and characterization of a new potyvirus infecting cucurbits
Perotto, Maria Cecilia
Potyviruses
Identification
Plant Viruses
Squashes
Potyvirus
Cucurbitaceae
Identificación
Virus de las Plantas
Calabazas
San Pedro, Buenos Aires
Illumina Sequencing
title_short Identification and characterization of a new potyvirus infecting cucurbits
title_full Identification and characterization of a new potyvirus infecting cucurbits
title_fullStr Identification and characterization of a new potyvirus infecting cucurbits
title_full_unstemmed Identification and characterization of a new potyvirus infecting cucurbits
title_sort Identification and characterization of a new potyvirus infecting cucurbits
dc.creator.none.fl_str_mv Perotto, Maria Cecilia
Pozzi, Elizabeth Alicia
Celli, Marcos Giovani
Luciani, Cecilia Elizabeth
Mitidieri, Mariel Silvina
Conci, Vilma Cecilia
author Perotto, Maria Cecilia
author_facet Perotto, Maria Cecilia
Pozzi, Elizabeth Alicia
Celli, Marcos Giovani
Luciani, Cecilia Elizabeth
Mitidieri, Mariel Silvina
Conci, Vilma Cecilia
author_role author
author2 Pozzi, Elizabeth Alicia
Celli, Marcos Giovani
Luciani, Cecilia Elizabeth
Mitidieri, Mariel Silvina
Conci, Vilma Cecilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Potyviruses
Identification
Plant Viruses
Squashes
Potyvirus
Cucurbitaceae
Identificación
Virus de las Plantas
Calabazas
San Pedro, Buenos Aires
Illumina Sequencing
topic Potyviruses
Identification
Plant Viruses
Squashes
Potyvirus
Cucurbitaceae
Identificación
Virus de las Plantas
Calabazas
San Pedro, Buenos Aires
Illumina Sequencing
dc.description.none.fl_txt_mv A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
Instituto de Patología Vegetal
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pozzi, E.A. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Luciani, Cecilia E. Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba; Argentina
Fil: Mitidieri, Mariel Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-18T15:21:25Z
2018-10-18T15:21:25Z
2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3632
https://link.springer.com/article/10.1007%2Fs00705-017-3660-2#citeas
0304-8608
1432-8798 (Online)
https://doi.org/10.1007/s00705-017-3660-2
url http://hdl.handle.net/20.500.12123/3632
https://link.springer.com/article/10.1007%2Fs00705-017-3660-2#citeas
https://doi.org/10.1007/s00705-017-3660-2
identifier_str_mv 0304-8608
1432-8798 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv San Pedro (inhabited place)
Buenos Aires (province)
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Archives of virology 163 (3) : 719–724. (March 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143506541707264
score 12.712165

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