Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes

Autores
Salloum, María Soraya; Menduni, Maria Florencia; Benavides, María Patricia; Larrauri, Mariana; Luna, Celina Mercedes; Silvente, Sonia Teresa
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Salloum, María Soraya. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Menduni, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fondo para la Investigación Científica y Tecnológica; Argentina
Fil: Benavides, María Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Larrauri, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina
Fuente
Symbiosis 76 (3) : 265–275. (November 2018)
Materia
Soja
Glycine Max
Poliaminas
Flavonoides
Genotipos
Micorrizas Arbusculares
Soybeans
Polyamines
Flavonoids
Genotypes
Arbuscular Mycorrhiza
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3675
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3675
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypesSalloum, María SorayaMenduni, Maria FlorenciaBenavides, María PatriciaLarrauri, MarianaLuna, Celina MercedesSilvente, Sonia TeresaSojaGlycine MaxPoliaminasFlavonoidesGenotiposMicorrizas ArbuscularesSoybeansPolyaminesFlavonoidsGenotypesArbuscular MycorrhizaModern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Salloum, María Soraya. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Menduni, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fondo para la Investigación Científica y Tecnológica; ArgentinaFil: Benavides, María Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Larrauri, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); ArgentinaSpringer2018-10-23T14:24:19Z2018-10-23T14:24:19Z2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3675https://link.springer.com/article/10.1007%2Fs13199-018-0558-z0334-51141878-7665 (Online)https://doi.org/10.1007/s13199-018-0558-zSymbiosis 76 (3) : 265–275. (November 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:20Zoai:localhost:20.500.12123/3675instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:20.865INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
title Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
spellingShingle Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
Salloum, María Soraya
Soja
Glycine Max
Poliaminas
Flavonoides
Genotipos
Micorrizas Arbusculares
Soybeans
Polyamines
Flavonoids
Genotypes
Arbuscular Mycorrhiza
title_short Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
title_full Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
title_fullStr Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
title_full_unstemmed Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
title_sort Polyamines and flavonoids : key compounds in mycorrhizal colonization of improved and unimproved soybean genotypes
dc.creator.none.fl_str_mv Salloum, María Soraya
Menduni, Maria Florencia
Benavides, María Patricia
Larrauri, Mariana
Luna, Celina Mercedes
Silvente, Sonia Teresa
author Salloum, María Soraya
author_facet Salloum, María Soraya
Menduni, Maria Florencia
Benavides, María Patricia
Larrauri, Mariana
Luna, Celina Mercedes
Silvente, Sonia Teresa
author_role author
author2 Menduni, Maria Florencia
Benavides, María Patricia
Larrauri, Mariana
Luna, Celina Mercedes
Silvente, Sonia Teresa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Soja
Glycine Max
Poliaminas
Flavonoides
Genotipos
Micorrizas Arbusculares
Soybeans
Polyamines
Flavonoids
Genotypes
Arbuscular Mycorrhiza
topic Soja
Glycine Max
Poliaminas
Flavonoides
Genotipos
Micorrizas Arbusculares
Soybeans
Polyamines
Flavonoids
Genotypes
Arbuscular Mycorrhiza
dc.description.none.fl_txt_mv Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Salloum, María Soraya. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Menduni, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Fondo para la Investigación Científica y Tecnológica; Argentina
Fil: Benavides, María Patricia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Larrauri, Mariana. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Luna, Celina Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Silvente, Sonia. Universidad Nacional de Chilecito. Instituto de Ambiente de Montaña y Regiones Áridas (IAMRA); Argentina
description Modern breeding programs might have caused a reduction in plant responsiveness to arbuscular mycorrhizal fungi (AMF). Flavonoids and polyamines (PAs) are hypothesized to play a role in this symbiosis. We tested the effects of them in AMF roots of improved (I-1) and unimproved (UI-4) soybean genotypes, under the hypothesis that domestication decreased their concentration in roots, affecting AMF colonization, particularly arbuscule formation. After 20 days of treatment, AMF roots of UI-4 genotype had greater amount of total flavonoids/phenols and PAs while in I-1 genotype no differences were observed between roots of mycorrhizal (M) and non mycorrhizal (NM) plants. Exogenous application of flavonoids led to an increase in arbuscules in both genotypes. Improved-1 genotype needed higher levels of flavonoids to reach the percentage of mycorrhization achieved by UI-4 control. In regard to PAs, mycorrhizal plants of both genotypes had higher endogenous concentration than NM plants, however, the highest concentration, especially of putrescine (put) was in UI-4 M genotype. To check the participation of put in symbiosis we used RNAi silencing methodology. Down regulation of the GmADC transcript, involved in put formation, had a profound negative effect on mycorrhizal colonization and also affected the normal development of the plant. By contrast, down regulation of GmDAO, in which ADC transcript was expressed, arbuscule formation was similar to control plant. Our results suggest that mycorrhizal colonization is affected by soybean domestication particularly arbuscule formation and this effect seems to be mediated by the endogenous roots levels of flavonoids and PAs, especially put.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-23T14:24:19Z
2018-10-23T14:24:19Z
2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3675
https://link.springer.com/article/10.1007%2Fs13199-018-0558-z
0334-5114
1878-7665 (Online)
https://doi.org/10.1007/s13199-018-0558-z
url http://hdl.handle.net/20.500.12123/3675
https://link.springer.com/article/10.1007%2Fs13199-018-0558-z
https://doi.org/10.1007/s13199-018-0558-z
identifier_str_mv 0334-5114
1878-7665 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Symbiosis 76 (3) : 265–275. (November 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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