Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids
- Autores
- Scervino, Jose Martin; Ponce, María Alejandra; Della Mónica, Ivana Florencia; Vierheilig, Horst; Ocampo, Juan Antonio; Godeas, Alicia Margarita
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We tested the effects of the flavonoid 3-methoxi-5,6,7,8-hydroxy-4'hydroxy flavone (NMHTV) isolated from shoots of non arbuscular mycorrhizal (AM) inoculated clover, and of the flavonoids 5,6,7,8-hydroxy-3-methoxy flavone (MH-1); 5,6,7,8-hydroxy-4'- hydroxy flavone (MH-2); and 5,7-hydroxy-3,4'-methoxy flavone (MH-3); isolated from AM clover (Trifolium repens) shoots, on spore germination, hyphal length, hyphal branches and the number of cluster of auxiliary cells or the number of secondary spores (Presymbiotic stage) and on the number of entry points and the percentage of AM colonized root of tomato (Lycopersicum esculentum) by the AM fungi Gigaspora rosea, Giaspora margarita, Glomus mosseae and Glomus intraradices (Symbiotic stage). Non significant effects of the flavonoids isolated from the shoot of mycorrhizal colonized clover on the presymbiotic and symbiotic stages of Gigaspora and Glomus endophytes were found. The flavonoid NMHTV isolated from non AM clover shoot, did not affect the percentage of germination of spores but significantly increased (P < 0.05) the other steps of the presymbiotic stage of Gi. margarita spores when 2 μM concentration was used. The symbiotic stage of Gi. margarita was also significantly increased when 2 μM of the flavonoid NMHTV was applied. This flavonoid had no effect on the presymbiotic development of G. mosseae, G. intraradices and Gi. rosea except when 8 μM concentration was used, which inhibited the hyphal length of Gi. rosea. These results suggest the possible implication of the flavonoid NMHTV in the susceptibility of tomato roots to the AM formation by Gi. margarita. The absence of stimulation of the AM presymbiotic and symbiotic stages in tomato by exogenous application of the newly synthesized flavonoids MH-1, MH-2, and MH-3, in clover shoots after AM colonization, indicated that the autorregulation of the AM symbiosis can be, at least partially, due to the disappearance of flavonoids in AM colonized plants that stimulated the AM symbiosis.
Fil: Scervino, Jose Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Ponce, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Della Mónica, Ivana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Vierheilig, Horst. Universitat Fur Bodenkultur Wien; Austria. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Ocampo, Juan Antonio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España
Fil: Godeas, Alicia Margarita. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
ARBUSCULAR MYCORRHIZAS
FLAVONOIDS
SIGNAL MOLECULES
SYMBIOSIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/98597
Ver los metadatos del registro completo
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Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoidsScervino, Jose MartinPonce, María AlejandraDella Mónica, Ivana FlorenciaVierheilig, HorstOcampo, Juan AntonioGodeas, Alicia MargaritaARBUSCULAR MYCORRHIZASFLAVONOIDSSIGNAL MOLECULESSYMBIOSIShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We tested the effects of the flavonoid 3-methoxi-5,6,7,8-hydroxy-4'hydroxy flavone (NMHTV) isolated from shoots of non arbuscular mycorrhizal (AM) inoculated clover, and of the flavonoids 5,6,7,8-hydroxy-3-methoxy flavone (MH-1); 5,6,7,8-hydroxy-4'- hydroxy flavone (MH-2); and 5,7-hydroxy-3,4'-methoxy flavone (MH-3); isolated from AM clover (Trifolium repens) shoots, on spore germination, hyphal length, hyphal branches and the number of cluster of auxiliary cells or the number of secondary spores (Presymbiotic stage) and on the number of entry points and the percentage of AM colonized root of tomato (Lycopersicum esculentum) by the AM fungi Gigaspora rosea, Giaspora margarita, Glomus mosseae and Glomus intraradices (Symbiotic stage). Non significant effects of the flavonoids isolated from the shoot of mycorrhizal colonized clover on the presymbiotic and symbiotic stages of Gigaspora and Glomus endophytes were found. The flavonoid NMHTV isolated from non AM clover shoot, did not affect the percentage of germination of spores but significantly increased (P < 0.05) the other steps of the presymbiotic stage of Gi. margarita spores when 2 μM concentration was used. The symbiotic stage of Gi. margarita was also significantly increased when 2 μM of the flavonoid NMHTV was applied. This flavonoid had no effect on the presymbiotic development of G. mosseae, G. intraradices and Gi. rosea except when 8 μM concentration was used, which inhibited the hyphal length of Gi. rosea. These results suggest the possible implication of the flavonoid NMHTV in the susceptibility of tomato roots to the AM formation by Gi. margarita. The absence of stimulation of the AM presymbiotic and symbiotic stages in tomato by exogenous application of the newly synthesized flavonoids MH-1, MH-2, and MH-3, in clover shoots after AM colonization, indicated that the autorregulation of the AM symbiosis can be, at least partially, due to the disappearance of flavonoids in AM colonized plants that stimulated the AM symbiosis.Fil: Scervino, Jose Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Ponce, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Della Mónica, Ivana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Vierheilig, Horst. Universitat Fur Bodenkultur Wien; Austria. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Ocampo, Juan Antonio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; EspañaFil: Godeas, Alicia Margarita. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSoc Chilena Ciencia Suelo2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98597Scervino, Jose Martin; Ponce, María Alejandra; Della Mónica, Ivana Florencia; Vierheilig, Horst; Ocampo, Juan Antonio; et al.; Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids; Soc Chilena Ciencia Suelo; REVISTA DE LA CIENCIA DEL SUELO Y NUTRICIóN VEGETAL; 9; 2; 12-2009; 102-1150718-2791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4067/S0718-27912009000200002info:eu-repo/semantics/altIdentifier/url/https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0718-27912009000200002&lng=es&nrm=isoinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:38Zoai:ri.conicet.gov.ar:11336/98597instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:38.318CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
title |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
spellingShingle |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids Scervino, Jose Martin ARBUSCULAR MYCORRHIZAS FLAVONOIDS SIGNAL MOLECULES SYMBIOSIS |
title_short |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
title_full |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
title_fullStr |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
title_full_unstemmed |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
title_sort |
Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids |
dc.creator.none.fl_str_mv |
Scervino, Jose Martin Ponce, María Alejandra Della Mónica, Ivana Florencia Vierheilig, Horst Ocampo, Juan Antonio Godeas, Alicia Margarita |
author |
Scervino, Jose Martin |
author_facet |
Scervino, Jose Martin Ponce, María Alejandra Della Mónica, Ivana Florencia Vierheilig, Horst Ocampo, Juan Antonio Godeas, Alicia Margarita |
author_role |
author |
author2 |
Ponce, María Alejandra Della Mónica, Ivana Florencia Vierheilig, Horst Ocampo, Juan Antonio Godeas, Alicia Margarita |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
ARBUSCULAR MYCORRHIZAS FLAVONOIDS SIGNAL MOLECULES SYMBIOSIS |
topic |
ARBUSCULAR MYCORRHIZAS FLAVONOIDS SIGNAL MOLECULES SYMBIOSIS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We tested the effects of the flavonoid 3-methoxi-5,6,7,8-hydroxy-4'hydroxy flavone (NMHTV) isolated from shoots of non arbuscular mycorrhizal (AM) inoculated clover, and of the flavonoids 5,6,7,8-hydroxy-3-methoxy flavone (MH-1); 5,6,7,8-hydroxy-4'- hydroxy flavone (MH-2); and 5,7-hydroxy-3,4'-methoxy flavone (MH-3); isolated from AM clover (Trifolium repens) shoots, on spore germination, hyphal length, hyphal branches and the number of cluster of auxiliary cells or the number of secondary spores (Presymbiotic stage) and on the number of entry points and the percentage of AM colonized root of tomato (Lycopersicum esculentum) by the AM fungi Gigaspora rosea, Giaspora margarita, Glomus mosseae and Glomus intraradices (Symbiotic stage). Non significant effects of the flavonoids isolated from the shoot of mycorrhizal colonized clover on the presymbiotic and symbiotic stages of Gigaspora and Glomus endophytes were found. The flavonoid NMHTV isolated from non AM clover shoot, did not affect the percentage of germination of spores but significantly increased (P < 0.05) the other steps of the presymbiotic stage of Gi. margarita spores when 2 μM concentration was used. The symbiotic stage of Gi. margarita was also significantly increased when 2 μM of the flavonoid NMHTV was applied. This flavonoid had no effect on the presymbiotic development of G. mosseae, G. intraradices and Gi. rosea except when 8 μM concentration was used, which inhibited the hyphal length of Gi. rosea. These results suggest the possible implication of the flavonoid NMHTV in the susceptibility of tomato roots to the AM formation by Gi. margarita. The absence of stimulation of the AM presymbiotic and symbiotic stages in tomato by exogenous application of the newly synthesized flavonoids MH-1, MH-2, and MH-3, in clover shoots after AM colonization, indicated that the autorregulation of the AM symbiosis can be, at least partially, due to the disappearance of flavonoids in AM colonized plants that stimulated the AM symbiosis. Fil: Scervino, Jose Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Ponce, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Della Mónica, Ivana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Vierheilig, Horst. Universitat Fur Bodenkultur Wien; Austria. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España Fil: Ocampo, Juan Antonio. Consejo Superior de Investigaciones Científicas. Estación Experimental del Zaidín; España Fil: Godeas, Alicia Margarita. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
We tested the effects of the flavonoid 3-methoxi-5,6,7,8-hydroxy-4'hydroxy flavone (NMHTV) isolated from shoots of non arbuscular mycorrhizal (AM) inoculated clover, and of the flavonoids 5,6,7,8-hydroxy-3-methoxy flavone (MH-1); 5,6,7,8-hydroxy-4'- hydroxy flavone (MH-2); and 5,7-hydroxy-3,4'-methoxy flavone (MH-3); isolated from AM clover (Trifolium repens) shoots, on spore germination, hyphal length, hyphal branches and the number of cluster of auxiliary cells or the number of secondary spores (Presymbiotic stage) and on the number of entry points and the percentage of AM colonized root of tomato (Lycopersicum esculentum) by the AM fungi Gigaspora rosea, Giaspora margarita, Glomus mosseae and Glomus intraradices (Symbiotic stage). Non significant effects of the flavonoids isolated from the shoot of mycorrhizal colonized clover on the presymbiotic and symbiotic stages of Gigaspora and Glomus endophytes were found. The flavonoid NMHTV isolated from non AM clover shoot, did not affect the percentage of germination of spores but significantly increased (P < 0.05) the other steps of the presymbiotic stage of Gi. margarita spores when 2 μM concentration was used. The symbiotic stage of Gi. margarita was also significantly increased when 2 μM of the flavonoid NMHTV was applied. This flavonoid had no effect on the presymbiotic development of G. mosseae, G. intraradices and Gi. rosea except when 8 μM concentration was used, which inhibited the hyphal length of Gi. rosea. These results suggest the possible implication of the flavonoid NMHTV in the susceptibility of tomato roots to the AM formation by Gi. margarita. The absence of stimulation of the AM presymbiotic and symbiotic stages in tomato by exogenous application of the newly synthesized flavonoids MH-1, MH-2, and MH-3, in clover shoots after AM colonization, indicated that the autorregulation of the AM symbiosis can be, at least partially, due to the disappearance of flavonoids in AM colonized plants that stimulated the AM symbiosis. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/98597 Scervino, Jose Martin; Ponce, María Alejandra; Della Mónica, Ivana Florencia; Vierheilig, Horst; Ocampo, Juan Antonio; et al.; Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids; Soc Chilena Ciencia Suelo; REVISTA DE LA CIENCIA DEL SUELO Y NUTRICIóN VEGETAL; 9; 2; 12-2009; 102-115 0718-2791 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/98597 |
identifier_str_mv |
Scervino, Jose Martin; Ponce, María Alejandra; Della Mónica, Ivana Florencia; Vierheilig, Horst; Ocampo, Juan Antonio; et al.; Development of arbuscular mycorrhizal fungi in the presence of different patterns of Trifolium repens shoot flavonoids; Soc Chilena Ciencia Suelo; REVISTA DE LA CIENCIA DEL SUELO Y NUTRICIóN VEGETAL; 9; 2; 12-2009; 102-115 0718-2791 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4067/S0718-27912009000200002 info:eu-repo/semantics/altIdentifier/url/https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0718-27912009000200002&lng=es&nrm=iso |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Soc Chilena Ciencia Suelo |
publisher.none.fl_str_mv |
Soc Chilena Ciencia Suelo |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613072420864000 |
score |
13.070432 |