Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2

Autores
Giovambattista, Guillermo; Bao, Aronggaowa; Marcuzzi, Olivia; Loza Vega, Ariel Jhonny; Pereira Rico, Juan A.; Ortega Masague, Maria Florencia; Castro Rojas, Liz Aurora; Martinez, Ruben Dario; Reinosa, Odalys Uffo; Aida, Yoko
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin American cattle remain limited. This study assessed BLV infection and proviral load in 244 animals from six native breeds: Argentine Creole (CrAr), Patagonian Argentine Creole (CrArPat), Pampa Chaqueño Creole (CrPaCh), Bolivian Creole from Cochabamba (CrCoch), Saavedreño Creole (CrSaa), and Siboney (Sib), sampled across Argentina, Bolivia, Paraguay, and Cuba. BLV-CoCoMo-qPCR-2 assay detected BLV provirus in 76 animals (31.1%), with a mean load of 9923 copies per 105 cells (range: 1–79,740). Infection rates varied significantly by breed (9.8% in CrAr to 83.8% in CrPaCh) and country (15.6% in Argentina to 83.8% in Paraguay) (p = 9.999 × 10−5). Among positives, 57.9% exhibited low proviral load (≤1000 copies), and 13.2% showed moderate levels (1001–9999), suggesting potential resistance to EBL progression. This is the first comprehensive report of BLV proviral load in Creole cattle across Latin America, offering novel epidemiological insights and highlighting the importance of native breeds in BLV surveillance.
EEA Famaillá
Fil: Giovambattista, Guillermo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria "Ing. Fernando N. Dulout”; Argentina
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; Argentina
Fil: Giovambattista, Guillermo. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; Japón
Fil: Bao, Aronggaowa. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; Japón
Fil: Marcuzzi, Olivia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; Argentina
Fil: Marcuzzi, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; Argentina
Fil: Loza Vega, Ariel Jhonny. Universidad Autónoma Gabriel René Moreno. Facultad de Ciencias Veterinarias; Bolivia
Fil: Pereira Rico, Juan A. Universidad Autónoma Gabriel René Moreno. Facultad de Ciencias Veterinarias; Bolivia
Fil: Ortega Masague, Maria Florencia. Estación Experimental Agropecuaria Famaillá. Agencia De Extensión Rural Lules; Argentina
Fil: Castro Rojas, Liz Aurora. Universidad Nacional de Asunción. Facultad de Ciencias Veterinarias; Paraguay
Fil: Martinez, Ruben Dario. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Reinosa, Odalys Uffo. Centro Nacional de Sanidad Agropecuaria (CENSA); Cuba
Fil: Aida, Yoko. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; Japón
Fuente
Pathogens 14 (10) : 1005. (October 2025)
Materia
Virus Leucemia Bovina
Ganado Bovino
Enfermedades de los Animales
Epidemiología
Bolivia (Estado Plurinacional de)
Bovine Leukemia Virus
Cattle
Animal Diseases
Epidemiology
PCR
Argentina
Bolivia (Plurinational State of)
Paraguay
Cuba
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24212
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24212
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2Giovambattista, GuillermoBao, AronggaowaMarcuzzi, OliviaLoza Vega, Ariel JhonnyPereira Rico, Juan A.Ortega Masague, Maria FlorenciaCastro Rojas, Liz AuroraMartinez, Ruben DarioReinosa, Odalys UffoAida, YokoVirus Leucemia BovinaGanado BovinoEnfermedades de los AnimalesEpidemiologíaBolivia (Estado Plurinacional de)Bovine Leukemia VirusCattleAnimal DiseasesEpidemiologyPCRArgentinaBolivia (Plurinational State of)ParaguayCubaBovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin American cattle remain limited. This study assessed BLV infection and proviral load in 244 animals from six native breeds: Argentine Creole (CrAr), Patagonian Argentine Creole (CrArPat), Pampa Chaqueño Creole (CrPaCh), Bolivian Creole from Cochabamba (CrCoch), Saavedreño Creole (CrSaa), and Siboney (Sib), sampled across Argentina, Bolivia, Paraguay, and Cuba. BLV-CoCoMo-qPCR-2 assay detected BLV provirus in 76 animals (31.1%), with a mean load of 9923 copies per 105 cells (range: 1–79,740). Infection rates varied significantly by breed (9.8% in CrAr to 83.8% in CrPaCh) and country (15.6% in Argentina to 83.8% in Paraguay) (p = 9.999 × 10−5). Among positives, 57.9% exhibited low proviral load (≤1000 copies), and 13.2% showed moderate levels (1001–9999), suggesting potential resistance to EBL progression. This is the first comprehensive report of BLV proviral load in Creole cattle across Latin America, offering novel epidemiological insights and highlighting the importance of native breeds in BLV surveillance.EEA FamailláFil: Giovambattista, Guillermo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria "Ing. Fernando N. Dulout”; ArgentinaFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; ArgentinaFil: Giovambattista, Guillermo. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; JapónFil: Bao, Aronggaowa. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; JapónFil: Marcuzzi, Olivia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; ArgentinaFil: Marcuzzi, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; ArgentinaFil: Loza Vega, Ariel Jhonny. Universidad Autónoma Gabriel René Moreno. Facultad de Ciencias Veterinarias; BoliviaFil: Pereira Rico, Juan A. Universidad Autónoma Gabriel René Moreno. Facultad de Ciencias Veterinarias; BoliviaFil: Ortega Masague, Maria Florencia. Estación Experimental Agropecuaria Famaillá. Agencia De Extensión Rural Lules; ArgentinaFil: Castro Rojas, Liz Aurora. Universidad Nacional de Asunción. Facultad de Ciencias Veterinarias; ParaguayFil: Martinez, Ruben Dario. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; ArgentinaFil: Reinosa, Odalys Uffo. Centro Nacional de Sanidad Agropecuaria (CENSA); CubaFil: Aida, Yoko. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; JapónMDPI2025-10-20T11:14:19Z2025-10-20T11:14:19Z2025-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24212https://www.mdpi.com/2076-0817/14/10/10052076-0817https://doi.org/10.3390/pathogens14101005Pathogens 14 (10) : 1005. (October 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-12-18T09:04:00Zoai:localhost:20.500.12123/24212instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-12-18 09:04:01.327INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
title Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
spellingShingle Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
Giovambattista, Guillermo
Virus Leucemia Bovina
Ganado Bovino
Enfermedades de los Animales
Epidemiología
Bolivia (Estado Plurinacional de)
Bovine Leukemia Virus
Cattle
Animal Diseases
Epidemiology
PCR
Argentina
Bolivia (Plurinational State of)
Paraguay
Cuba
title_short Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
title_full Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
title_fullStr Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
title_full_unstemmed Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
title_sort Detection of Bovine Leukemia Virus in Argentine, Bolivian, Paraguayan and Cuban Native Cattle Using a Quantitative Real-Time PCR Assay-BLV-CoCoMo-qPCR-2
dc.creator.none.fl_str_mv Giovambattista, Guillermo
Bao, Aronggaowa
Marcuzzi, Olivia
Loza Vega, Ariel Jhonny
Pereira Rico, Juan A.
Ortega Masague, Maria Florencia
Castro Rojas, Liz Aurora
Martinez, Ruben Dario
Reinosa, Odalys Uffo
Aida, Yoko
author Giovambattista, Guillermo
author_facet Giovambattista, Guillermo
Bao, Aronggaowa
Marcuzzi, Olivia
Loza Vega, Ariel Jhonny
Pereira Rico, Juan A.
Ortega Masague, Maria Florencia
Castro Rojas, Liz Aurora
Martinez, Ruben Dario
Reinosa, Odalys Uffo
Aida, Yoko
author_role author
author2 Bao, Aronggaowa
Marcuzzi, Olivia
Loza Vega, Ariel Jhonny
Pereira Rico, Juan A.
Ortega Masague, Maria Florencia
Castro Rojas, Liz Aurora
Martinez, Ruben Dario
Reinosa, Odalys Uffo
Aida, Yoko
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Virus Leucemia Bovina
Ganado Bovino
Enfermedades de los Animales
Epidemiología
Bolivia (Estado Plurinacional de)
Bovine Leukemia Virus
Cattle
Animal Diseases
Epidemiology
PCR
Argentina
Bolivia (Plurinational State of)
Paraguay
Cuba
topic Virus Leucemia Bovina
Ganado Bovino
Enfermedades de los Animales
Epidemiología
Bolivia (Estado Plurinacional de)
Bovine Leukemia Virus
Cattle
Animal Diseases
Epidemiology
PCR
Argentina
Bolivia (Plurinational State of)
Paraguay
Cuba
dc.description.none.fl_txt_mv Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin American cattle remain limited. This study assessed BLV infection and proviral load in 244 animals from six native breeds: Argentine Creole (CrAr), Patagonian Argentine Creole (CrArPat), Pampa Chaqueño Creole (CrPaCh), Bolivian Creole from Cochabamba (CrCoch), Saavedreño Creole (CrSaa), and Siboney (Sib), sampled across Argentina, Bolivia, Paraguay, and Cuba. BLV-CoCoMo-qPCR-2 assay detected BLV provirus in 76 animals (31.1%), with a mean load of 9923 copies per 105 cells (range: 1–79,740). Infection rates varied significantly by breed (9.8% in CrAr to 83.8% in CrPaCh) and country (15.6% in Argentina to 83.8% in Paraguay) (p = 9.999 × 10−5). Among positives, 57.9% exhibited low proviral load (≤1000 copies), and 13.2% showed moderate levels (1001–9999), suggesting potential resistance to EBL progression. This is the first comprehensive report of BLV proviral load in Creole cattle across Latin America, offering novel epidemiological insights and highlighting the importance of native breeds in BLV surveillance.
EEA Famaillá
Fil: Giovambattista, Guillermo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria "Ing. Fernando N. Dulout”; Argentina
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; Argentina
Fil: Giovambattista, Guillermo. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; Japón
Fil: Bao, Aronggaowa. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; Japón
Fil: Marcuzzi, Olivia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; Argentina
Fil: Marcuzzi, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Genética Veterinaria ‘Ing. Fernando N. Dulout’; Argentina
Fil: Loza Vega, Ariel Jhonny. Universidad Autónoma Gabriel René Moreno. Facultad de Ciencias Veterinarias; Bolivia
Fil: Pereira Rico, Juan A. Universidad Autónoma Gabriel René Moreno. Facultad de Ciencias Veterinarias; Bolivia
Fil: Ortega Masague, Maria Florencia. Estación Experimental Agropecuaria Famaillá. Agencia De Extensión Rural Lules; Argentina
Fil: Castro Rojas, Liz Aurora. Universidad Nacional de Asunción. Facultad de Ciencias Veterinarias; Paraguay
Fil: Martinez, Ruben Dario. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
Fil: Reinosa, Odalys Uffo. Centro Nacional de Sanidad Agropecuaria (CENSA); Cuba
Fil: Aida, Yoko. The University of Tokyo. Graduate School of Agricultural and Life Sciences. Laboratory of Global Infectious Diseases Control Science; Japón
description Bovine leukemia virus (BLV), an oncogenic retrovirus of the genus Deltaretrovirus, causes enzootic bovine leukosis (EBL), the most prevalent neoplastic disease in cattle and a major source of economic loss. While BLV prevalence has been studied in commercial breeds, data on native Latin American cattle remain limited. This study assessed BLV infection and proviral load in 244 animals from six native breeds: Argentine Creole (CrAr), Patagonian Argentine Creole (CrArPat), Pampa Chaqueño Creole (CrPaCh), Bolivian Creole from Cochabamba (CrCoch), Saavedreño Creole (CrSaa), and Siboney (Sib), sampled across Argentina, Bolivia, Paraguay, and Cuba. BLV-CoCoMo-qPCR-2 assay detected BLV provirus in 76 animals (31.1%), with a mean load of 9923 copies per 105 cells (range: 1–79,740). Infection rates varied significantly by breed (9.8% in CrAr to 83.8% in CrPaCh) and country (15.6% in Argentina to 83.8% in Paraguay) (p = 9.999 × 10−5). Among positives, 57.9% exhibited low proviral load (≤1000 copies), and 13.2% showed moderate levels (1001–9999), suggesting potential resistance to EBL progression. This is the first comprehensive report of BLV proviral load in Creole cattle across Latin America, offering novel epidemiological insights and highlighting the importance of native breeds in BLV surveillance.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-20T11:14:19Z
2025-10-20T11:14:19Z
2025-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24212
https://www.mdpi.com/2076-0817/14/10/1005
2076-0817
https://doi.org/10.3390/pathogens14101005
url http://hdl.handle.net/20.500.12123/24212
https://www.mdpi.com/2076-0817/14/10/1005
https://doi.org/10.3390/pathogens14101005
identifier_str_mv 2076-0817
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Pathogens 14 (10) : 1005. (October 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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