Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms

Autores
Hasenauer, Flavia Carolina; Caffaro, María Eugenia; Czibener, Cecilia; Comerci, Diego José; Poli, Mario Andres; Rossetti, Carlos Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis of the two microsatellites present at the 3′UTR SLC11A1 gene in 254 Bos taurus purebred, 125 B. indicus purebred and 54 B. taurus × B. indicus crossbred cattle. The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). The alleles 159 and 175 were the most frequent in all breeds analyzed. B. taurus showed the most homogeneous haplotype and genotype for both microsatellites, whereas B. indicus showed the most heterogeneous haplotype and genotype. Two novel variants (alleles 161 and 163) within the MS1 are reported as well as novel variants in MS2 in Holstein breed. The knowledge of the polymorphisms distribution in both microsatellites at the 3′UTR of the SLC11A1 gene in cattle breeds is useful for future experimental design to evaluate the association between reported genotypes and natural resistance to pathogens infection.
Instituto de Patobiología
Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fuente
Molecular Biology Reports 40 (1) : 545–552 (January 2013)
Materia
Genética
Genes
Ganado Bovino
Polimorfismo
Microsatélites
Resistencia Genética
Genetics
Cattle
Polymorphism
Microsatellites
Genetic Resistance
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4274
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4274
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network_name_str INTA Digital (INTA)
spelling Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphismsHasenauer, Flavia CarolinaCaffaro, María EugeniaCzibener, CeciliaComerci, Diego JoséPoli, Mario AndresRossetti, Carlos AlbertoGenéticaGenesGanado BovinoPolimorfismoMicrosatélitesResistencia GenéticaGeneticsCattlePolymorphismMicrosatellitesGenetic ResistancePolymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis of the two microsatellites present at the 3′UTR SLC11A1 gene in 254 Bos taurus purebred, 125 B. indicus purebred and 54 B. taurus × B. indicus crossbred cattle. The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). The alleles 159 and 175 were the most frequent in all breeds analyzed. B. taurus showed the most homogeneous haplotype and genotype for both microsatellites, whereas B. indicus showed the most heterogeneous haplotype and genotype. Two novel variants (alleles 161 and 163) within the MS1 are reported as well as novel variants in MS2 in Holstein breed. The knowledge of the polymorphisms distribution in both microsatellites at the 3′UTR of the SLC11A1 gene in cattle breeds is useful for future experimental design to evaluate the association between reported genotypes and natural resistance to pathogens infection.Instituto de PatobiologíaFil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaSpringer2019-01-16T14:26:41Z2019-01-16T14:26:41Z2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s11033-012-2091-0http://hdl.handle.net/20.500.12123/42740301-48511573-4978https://doi.org/10.1007/s11033-012-2091-0Molecular Biology Reports 40 (1) : 545–552 (January 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:46Zoai:localhost:20.500.12123/4274instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:46.811INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
title Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
spellingShingle Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
Hasenauer, Flavia Carolina
Genética
Genes
Ganado Bovino
Polimorfismo
Microsatélites
Resistencia Genética
Genetics
Cattle
Polymorphism
Microsatellites
Genetic Resistance
title_short Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
title_full Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
title_fullStr Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
title_full_unstemmed Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
title_sort Genetic analysis of the 3′ untranslated region of the bovine SLC11A1 gene reveals novel polymorphisms
dc.creator.none.fl_str_mv Hasenauer, Flavia Carolina
Caffaro, María Eugenia
Czibener, Cecilia
Comerci, Diego José
Poli, Mario Andres
Rossetti, Carlos Alberto
author Hasenauer, Flavia Carolina
author_facet Hasenauer, Flavia Carolina
Caffaro, María Eugenia
Czibener, Cecilia
Comerci, Diego José
Poli, Mario Andres
Rossetti, Carlos Alberto
author_role author
author2 Caffaro, María Eugenia
Czibener, Cecilia
Comerci, Diego José
Poli, Mario Andres
Rossetti, Carlos Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Genes
Ganado Bovino
Polimorfismo
Microsatélites
Resistencia Genética
Genetics
Cattle
Polymorphism
Microsatellites
Genetic Resistance
topic Genética
Genes
Ganado Bovino
Polimorfismo
Microsatélites
Resistencia Genética
Genetics
Cattle
Polymorphism
Microsatellites
Genetic Resistance
dc.description.none.fl_txt_mv Polymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis of the two microsatellites present at the 3′UTR SLC11A1 gene in 254 Bos taurus purebred, 125 B. indicus purebred and 54 B. taurus × B. indicus crossbred cattle. The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). The alleles 159 and 175 were the most frequent in all breeds analyzed. B. taurus showed the most homogeneous haplotype and genotype for both microsatellites, whereas B. indicus showed the most heterogeneous haplotype and genotype. Two novel variants (alleles 161 and 163) within the MS1 are reported as well as novel variants in MS2 in Holstein breed. The knowledge of the polymorphisms distribution in both microsatellites at the 3′UTR of the SLC11A1 gene in cattle breeds is useful for future experimental design to evaluate the association between reported genotypes and natural resistance to pathogens infection.
Instituto de Patobiología
Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Czibener, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Comerci, Diego José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
description Polymorphisms in microsatellites at the 3′ untranslated region (3′UTR) of the SLC11A1 (solute carrier family 11 member A1) gene have been associated with natural resistance to Brucella abortus and Mycobacterium bovis infection in livestock species. Here, we carried out an individual genetic analysis of the two microsatellites present at the 3′UTR SLC11A1 gene in 254 Bos taurus purebred, 125 B. indicus purebred and 54 B. taurus × B. indicus crossbred cattle. The genotyping by capillary electrophoresis showed the presence of four alleles (157, 159, 161 and 163) for the first microsatellite (MS1) and six alleles (175, 177, 179, 181, 183 and 185) for the second microsatellite (MS2). The alleles 159 and 175 were the most frequent in all breeds analyzed. B. taurus showed the most homogeneous haplotype and genotype for both microsatellites, whereas B. indicus showed the most heterogeneous haplotype and genotype. Two novel variants (alleles 161 and 163) within the MS1 are reported as well as novel variants in MS2 in Holstein breed. The knowledge of the polymorphisms distribution in both microsatellites at the 3′UTR of the SLC11A1 gene in cattle breeds is useful for future experimental design to evaluate the association between reported genotypes and natural resistance to pathogens infection.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
2019-01-16T14:26:41Z
2019-01-16T14:26:41Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s11033-012-2091-0
http://hdl.handle.net/20.500.12123/4274
0301-4851
1573-4978
https://doi.org/10.1007/s11033-012-2091-0
url https://link.springer.com/article/10.1007/s11033-012-2091-0
http://hdl.handle.net/20.500.12123/4274
https://doi.org/10.1007/s11033-012-2091-0
identifier_str_mv 0301-4851
1573-4978
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Molecular Biology Reports 40 (1) : 545–552 (January 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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