Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats

Autores
Iacoboni, Paola Anabella; Hasenauer, Flavia Carolina; Caffaro, María Eugenia; Gaido, Analia Beatriz; Rossetto, Cristina; Neumann, Roberto Daniel; Salatin, Antonio Omar; Bertoni, Emiliano Agustín; Poli, Mario Andres; Rossetti, Carlos Alberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.
Instituto de Patobiología
Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Neumann, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Salatin, Antonio Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Bertoni, Emiliano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fuente
Veterinary Immunology and Immunopathology 160 (3–4) : 230-234 (August 2014)
Materia
Caprinos
Genética
Polimorfismo
Brucella
Brucelosis
Goats
Genetics
Polymorphism
Brucellosis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4315
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4315
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goatsIacoboni, Paola AnabellaHasenauer, Flavia CarolinaCaffaro, María EugeniaGaido, Analia BeatrizRossetto, CristinaNeumann, Roberto DanielSalatin, Antonio OmarBertoni, Emiliano AgustínPoli, Mario AndresRossetti, Carlos AlbertoCaprinosGenéticaPolimorfismoBrucellaBrucelosisGoatsGeneticsPolymorphismBrucellosisGoats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.Instituto de PatobiologíaFil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; ArgentinaFil: Neumann, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Salatin, Antonio Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Bertoni, Emiliano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaElsevier2019-01-23T13:01:40Z2019-01-23T13:01:40Z2014-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0165242714001202http://hdl.handle.net/20.500.12123/43150165-2427https://doi.org/10.1016/j.vetimm.2014.05.007Veterinary Immunology and Immunopathology 160 (3–4) : 230-234 (August 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:48Zoai:localhost:20.500.12123/4315instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:49.251INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
spellingShingle Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
Iacoboni, Paola Anabella
Caprinos
Genética
Polimorfismo
Brucella
Brucelosis
Goats
Genetics
Polymorphism
Brucellosis
title_short Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_full Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_fullStr Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_full_unstemmed Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
title_sort Polymorphisms at the 3′ untranslated region of SLC11A1 gene are associated with protection to Brucella infection in goats
dc.creator.none.fl_str_mv Iacoboni, Paola Anabella
Hasenauer, Flavia Carolina
Caffaro, María Eugenia
Gaido, Analia Beatriz
Rossetto, Cristina
Neumann, Roberto Daniel
Salatin, Antonio Omar
Bertoni, Emiliano Agustín
Poli, Mario Andres
Rossetti, Carlos Alberto
author Iacoboni, Paola Anabella
author_facet Iacoboni, Paola Anabella
Hasenauer, Flavia Carolina
Caffaro, María Eugenia
Gaido, Analia Beatriz
Rossetto, Cristina
Neumann, Roberto Daniel
Salatin, Antonio Omar
Bertoni, Emiliano Agustín
Poli, Mario Andres
Rossetti, Carlos Alberto
author_role author
author2 Hasenauer, Flavia Carolina
Caffaro, María Eugenia
Gaido, Analia Beatriz
Rossetto, Cristina
Neumann, Roberto Daniel
Salatin, Antonio Omar
Bertoni, Emiliano Agustín
Poli, Mario Andres
Rossetti, Carlos Alberto
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Caprinos
Genética
Polimorfismo
Brucella
Brucelosis
Goats
Genetics
Polymorphism
Brucellosis
topic Caprinos
Genética
Polimorfismo
Brucella
Brucelosis
Goats
Genetics
Polymorphism
Brucellosis
dc.description.none.fl_txt_mv Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.
Instituto de Patobiología
Fil: Iacoboni, Paola Anabella. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caffaro, Marí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Gaido, Analía Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Rossetto, Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Neumann, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Salatin, Antonio Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Bertoni, Emiliano Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
description Goats are susceptible to brucellosis and the detection of Brucella-infected animals is carried out by serological tests. In other ruminant species, polymorphisms in microsatellites (Ms) of 3′ untranslated region (3′UTR) of the solute carrier family 11 member A1 (SLC11A1) gene were associated with resistance to Brucella abortus infection. Goats present two polymorphic Ms at the 3′UTR end of SLC11A1 gene, called regions A and B. Here, we evaluated if polymorphisms in regions A and/or B are associated with Brucella infection in goats. Serum (for the detection of Brucella-specific antibodies) and hair samples (for DNA isolation and structure analysis of the SLC11A1 gene) were randomly collected from 229 adult native goats from the northwest of Argentina. Serological status was evaluated by buffer plate antigen test (BPAT) complemented by the fluorescent polarization assay (FPA), and the genotype of the 3′UTR of the SLC11A1 gene was determined by capillary electrophoresis and confirmed by sequence analysis. Polymorphisms in regions A and B of the 3′UTR SLC11A1 gene were found statistically significant associated with protection to Brucella infection. Specifically, the association study indicates statistical significance of the allele A15 and B7/B7 genotype with absence of Brucella-specific antibodies (p = 0.0003 and 0.0088, respectively). These data open a promising opportunity for limiting goat brucellosis through selective breeding of animals based on genetic markers associated with natural resistance to B. melitensis infection.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-15
2019-01-23T13:01:40Z
2019-01-23T13:01:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0165242714001202
http://hdl.handle.net/20.500.12123/4315
0165-2427
https://doi.org/10.1016/j.vetimm.2014.05.007
url https://www.sciencedirect.com/science/article/pii/S0165242714001202
http://hdl.handle.net/20.500.12123/4315
https://doi.org/10.1016/j.vetimm.2014.05.007
identifier_str_mv 0165-2427
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 160 (3–4) : 230-234 (August 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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