Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas

Autores
Martínez, Diana; Thompson, Carolina Soledad; Draghi, Maria Graciela; Canavesio, Vilma; Jacobo, Roberto; Zimmer, Patricia Andrea; Elena, Sebastián Alejandro; Nicola, Ana M.; Torioni, Susana Marta
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.
EEA Rafaela
Fil: Martinez, Diana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Draghi, Maria Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Canavesio, Vilma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Jacobo, Roberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Elena, Sebastián. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentina
Fil: Nicola, Ana M. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentina
Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fuente
Veterinary Microbiology 173 (1–2) : 172-176 (September 2014)
Materia
Búfalo de Agua
Brucella Abortus
Genotipos
Fenotipos
Brucelosis
Water Buffaloes
Genotypes
Phenotypes
Brucellosis
América Latina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2688
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2688
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the AmericasMartínez, DianaThompson, Carolina SoledadDraghi, Maria GracielaCanavesio, VilmaJacobo, RobertoZimmer, Patricia AndreaElena, Sebastián AlejandroNicola, Ana M.Torioni, Susana MartaBúfalo de AguaBrucella AbortusGenotiposFenotiposBrucelosisWater BuffaloesGenotypesPhenotypesBrucellosisAmérica LatinaAn isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.EEA RafaelaFil: Martinez, Diana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Draghi, Maria Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Canavesio, Vilma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Jacobo, Roberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Elena, Sebastián. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; ArgentinaFil: Nicola, Ana M. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; ArgentinaFil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina2018-06-28T13:59:03Z2018-06-28T13:59:03Z2014-09-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2688https://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub0378-11351873-2542https://doi.org/10.1016/j.vetmic.2014.07.011Veterinary Microbiology 173 (1–2) : 172-176 (September 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:13Zoai:localhost:20.500.12123/2688instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:13.557INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
title Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
spellingShingle Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
Martínez, Diana
Búfalo de Agua
Brucella Abortus
Genotipos
Fenotipos
Brucelosis
Water Buffaloes
Genotypes
Phenotypes
Brucellosis
América Latina
title_short Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
title_full Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
title_fullStr Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
title_full_unstemmed Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
title_sort Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas
dc.creator.none.fl_str_mv Martínez, Diana
Thompson, Carolina Soledad
Draghi, Maria Graciela
Canavesio, Vilma
Jacobo, Roberto
Zimmer, Patricia Andrea
Elena, Sebastián Alejandro
Nicola, Ana M.
Torioni, Susana Marta
author Martínez, Diana
author_facet Martínez, Diana
Thompson, Carolina Soledad
Draghi, Maria Graciela
Canavesio, Vilma
Jacobo, Roberto
Zimmer, Patricia Andrea
Elena, Sebastián Alejandro
Nicola, Ana M.
Torioni, Susana Marta
author_role author
author2 Thompson, Carolina Soledad
Draghi, Maria Graciela
Canavesio, Vilma
Jacobo, Roberto
Zimmer, Patricia Andrea
Elena, Sebastián Alejandro
Nicola, Ana M.
Torioni, Susana Marta
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Búfalo de Agua
Brucella Abortus
Genotipos
Fenotipos
Brucelosis
Water Buffaloes
Genotypes
Phenotypes
Brucellosis
América Latina
topic Búfalo de Agua
Brucella Abortus
Genotipos
Fenotipos
Brucelosis
Water Buffaloes
Genotypes
Phenotypes
Brucellosis
América Latina
dc.description.none.fl_txt_mv An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.
EEA Rafaela
Fil: Martinez, Diana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Draghi, Maria Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Canavesio, Vilma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Jacobo, Roberto. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Zimmer, Patricia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Elena, Sebastián. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentina
Fil: Nicola, Ana M. OIE-FAO. Laboratorio y Centro de Referencia para Brucelosis; Argentina
Fil: Torioni, Susana Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
description An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus–B. melitensis–B. ovis–B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498 bp) and B. melitensis (731 bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-17
2018-06-28T13:59:03Z
2018-06-28T13:59:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2688
https://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub
0378-1135
1873-2542
https://doi.org/10.1016/j.vetmic.2014.07.011
url http://hdl.handle.net/20.500.12123/2688
https://www.sciencedirect.com/science/article/pii/S0378113514003344?via%3Dihub
https://doi.org/10.1016/j.vetmic.2014.07.011
identifier_str_mv 0378-1135
1873-2542
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Veterinary Microbiology 173 (1–2) : 172-176 (September 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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