Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina

Autores
Martinez, Diana; Thompson, Carolina; Russo, Ana Maria; Jacobo, Roberto; Torioni de Echaide, Susana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Buffalo (Bubalus bubalis) populations are spread across northern Argentina, and they share their habitat with bovines. Both species are susceptible to brucellosis, and they are under a National Plan of Control and Eradication. To characterize the Brucella spp. that infects buffaloes, the blood of 35 animals that tested positive to brucellosis by a complement fixation test was collected. DNA was obtained and analyzed by polymerase chain reaction using different molecular markers. The genera, species, and biovars of Brucella were established by analyzing specific regions of the genes omp31, eri, alkB, and omp2ab. Brucella spp. was identified in 15 of 35 tested buffaloes. The product of the omp31 gene identified the genera. The detection of two fragments of 297 bp and/or 1000 bp from the eri gene confirmed the presence of B. abortus S19 and wild-type B. abortus. The amplification of the alkB gene allowed the identification of B. abortus biovars characterized by fragments of 498 bp (bv1, bv2, or bv4). The simultaneous amplification of 498 bp (alkB) and 1000 bp (eri) products suggested the presence of B. abortus bv1, which is highly prevalent in the cattle of Argentina. Fragments of 827 bp and 857 bp were amplified from the omp2ab gene, and their sequences showed 100% identity with B. melitensis and B. abortus bv5 (GenBank). However, the 721 bp product (alkB) specific for B. melitensis could not be amplified. This is the first report indicating the presence of B. abortus bv5 in Latin America.
Fil: Martinez, Diana. Universidad Nacional del Nordeste; Argentina
Fil: Thompson, Carolina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
Fil: Russo, Ana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Formosa; Argentina
Fil: Jacobo, Roberto. Universidad Nacional del Nordeste; Argentina
Fil: Torioni de Echaide, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
Materia
BRUCELLOSIS
WATER BUFFALO
MOLECULAR TYPIFICATION
BRUCELLA ABORTUS BV 5
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8544
Acceder
id CONICETDig_59a6e11650079a0a54be736b26c07923
oai_identifier_str oai:ri.conicet.gov.ar:11336/8544
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast ArgentinaMartinez, DianaThompson, CarolinaRusso, Ana MariaJacobo, RobertoTorioni de Echaide, SusanaBRUCELLOSISWATER BUFFALOMOLECULAR TYPIFICATIONBRUCELLA ABORTUS BV 5https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Buffalo (Bubalus bubalis) populations are spread across northern Argentina, and they share their habitat with bovines. Both species are susceptible to brucellosis, and they are under a National Plan of Control and Eradication. To characterize the Brucella spp. that infects buffaloes, the blood of 35 animals that tested positive to brucellosis by a complement fixation test was collected. DNA was obtained and analyzed by polymerase chain reaction using different molecular markers. The genera, species, and biovars of Brucella were established by analyzing specific regions of the genes omp31, eri, alkB, and omp2ab. Brucella spp. was identified in 15 of 35 tested buffaloes. The product of the omp31 gene identified the genera. The detection of two fragments of 297 bp and/or 1000 bp from the eri gene confirmed the presence of B. abortus S19 and wild-type B. abortus. The amplification of the alkB gene allowed the identification of B. abortus biovars characterized by fragments of 498 bp (bv1, bv2, or bv4). The simultaneous amplification of 498 bp (alkB) and 1000 bp (eri) products suggested the presence of B. abortus bv1, which is highly prevalent in the cattle of Argentina. Fragments of 827 bp and 857 bp were amplified from the omp2ab gene, and their sequences showed 100% identity with B. melitensis and B. abortus bv5 (GenBank). However, the 721 bp product (alkB) specific for B. melitensis could not be amplified. This is the first report indicating the presence of B. abortus bv5 in Latin America.Fil: Martinez, Diana. Universidad Nacional del Nordeste; ArgentinaFil: Thompson, Carolina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; ArgentinaFil: Russo, Ana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Formosa; ArgentinaFil: Jacobo, Roberto. Universidad Nacional del Nordeste; ArgentinaFil: Torioni de Echaide, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; ArgentinaInt Buffalo Information Ctr2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8544Martinez, Diana; Thompson, Carolina; Russo, Ana Maria; Jacobo, Roberto; Torioni de Echaide, Susana; Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina; Int Buffalo Information Ctr; Buffallo Bulletin; 32; 2; 2-2014; 1029-10320125-6726enginfo:eu-repo/semantics/altIdentifier/url/cabdirect.org/abstracts/20143084085.htmlinfo:eu-repo/semantics/altIdentifier/url/http://ibic.lib.ku.ac.th/e-Bulletin/IBBUSI201302173.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:01:19Zoai:ri.conicet.gov.ar:11336/8544instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:01:19.708CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
title Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
spellingShingle Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
Martinez, Diana
BRUCELLOSIS
WATER BUFFALO
MOLECULAR TYPIFICATION
BRUCELLA ABORTUS BV 5
title_short Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
title_full Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
title_fullStr Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
title_full_unstemmed Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
title_sort Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina
dc.creator.none.fl_str_mv Martinez, Diana
Thompson, Carolina
Russo, Ana Maria
Jacobo, Roberto
Torioni de Echaide, Susana
author Martinez, Diana
author_facet Martinez, Diana
Thompson, Carolina
Russo, Ana Maria
Jacobo, Roberto
Torioni de Echaide, Susana
author_role author
author2 Thompson, Carolina
Russo, Ana Maria
Jacobo, Roberto
Torioni de Echaide, Susana
author2_role author
author
author
author
dc.subject.none.fl_str_mv BRUCELLOSIS
WATER BUFFALO
MOLECULAR TYPIFICATION
BRUCELLA ABORTUS BV 5
topic BRUCELLOSIS
WATER BUFFALO
MOLECULAR TYPIFICATION
BRUCELLA ABORTUS BV 5
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Buffalo (Bubalus bubalis) populations are spread across northern Argentina, and they share their habitat with bovines. Both species are susceptible to brucellosis, and they are under a National Plan of Control and Eradication. To characterize the Brucella spp. that infects buffaloes, the blood of 35 animals that tested positive to brucellosis by a complement fixation test was collected. DNA was obtained and analyzed by polymerase chain reaction using different molecular markers. The genera, species, and biovars of Brucella were established by analyzing specific regions of the genes omp31, eri, alkB, and omp2ab. Brucella spp. was identified in 15 of 35 tested buffaloes. The product of the omp31 gene identified the genera. The detection of two fragments of 297 bp and/or 1000 bp from the eri gene confirmed the presence of B. abortus S19 and wild-type B. abortus. The amplification of the alkB gene allowed the identification of B. abortus biovars characterized by fragments of 498 bp (bv1, bv2, or bv4). The simultaneous amplification of 498 bp (alkB) and 1000 bp (eri) products suggested the presence of B. abortus bv1, which is highly prevalent in the cattle of Argentina. Fragments of 827 bp and 857 bp were amplified from the omp2ab gene, and their sequences showed 100% identity with B. melitensis and B. abortus bv5 (GenBank). However, the 721 bp product (alkB) specific for B. melitensis could not be amplified. This is the first report indicating the presence of B. abortus bv5 in Latin America.
Fil: Martinez, Diana. Universidad Nacional del Nordeste; Argentina
Fil: Thompson, Carolina. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
Fil: Russo, Ana Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro de Investigaciones y Transferencia de Formosa; Argentina
Fil: Jacobo, Roberto. Universidad Nacional del Nordeste; Argentina
Fil: Torioni de Echaide, Susana. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
description Buffalo (Bubalus bubalis) populations are spread across northern Argentina, and they share their habitat with bovines. Both species are susceptible to brucellosis, and they are under a National Plan of Control and Eradication. To characterize the Brucella spp. that infects buffaloes, the blood of 35 animals that tested positive to brucellosis by a complement fixation test was collected. DNA was obtained and analyzed by polymerase chain reaction using different molecular markers. The genera, species, and biovars of Brucella were established by analyzing specific regions of the genes omp31, eri, alkB, and omp2ab. Brucella spp. was identified in 15 of 35 tested buffaloes. The product of the omp31 gene identified the genera. The detection of two fragments of 297 bp and/or 1000 bp from the eri gene confirmed the presence of B. abortus S19 and wild-type B. abortus. The amplification of the alkB gene allowed the identification of B. abortus biovars characterized by fragments of 498 bp (bv1, bv2, or bv4). The simultaneous amplification of 498 bp (alkB) and 1000 bp (eri) products suggested the presence of B. abortus bv1, which is highly prevalent in the cattle of Argentina. Fragments of 827 bp and 857 bp were amplified from the omp2ab gene, and their sequences showed 100% identity with B. melitensis and B. abortus bv5 (GenBank). However, the 721 bp product (alkB) specific for B. melitensis could not be amplified. This is the first report indicating the presence of B. abortus bv5 in Latin America.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8544
Martinez, Diana; Thompson, Carolina; Russo, Ana Maria; Jacobo, Roberto; Torioni de Echaide, Susana; Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina; Int Buffalo Information Ctr; Buffallo Bulletin; 32; 2; 2-2014; 1029-1032
0125-6726
url http://hdl.handle.net/11336/8544
identifier_str_mv Martinez, Diana; Thompson, Carolina; Russo, Ana Maria; Jacobo, Roberto; Torioni de Echaide, Susana; Molecular Identification of Brucella Abortus Bv5 and Strain 19 in Water Buffaloes (Bubalus Bubalis) in Northeast Argentina; Int Buffalo Information Ctr; Buffallo Bulletin; 32; 2; 2-2014; 1029-1032
0125-6726
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/cabdirect.org/abstracts/20143084085.html
info:eu-repo/semantics/altIdentifier/url/http://ibic.lib.ku.ac.th/e-Bulletin/IBBUSI201302173.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Int Buffalo Information Ctr
publisher.none.fl_str_mv Int Buffalo Information Ctr
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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