Molecular characterization of Cryptosporidium isolates from calves in Argentina

Autores
Tomazic, Mariela Luján; Maidana, Jimena; Dominguez, Mariana Gabriela; Louge Uriarte, Enrique Leopoldo; Galarza, Roxana Ivon; Garro, Carlos Javier; Florin-Christensen, Monica; Schnittger, Leonhard
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Cryptosporidiosis is responsible for significant fatalities of neonatal calves, resulting in substantial economic loss in dairy farming in several countries. Additionally, the high shedding of environmentally resistant oocysts by calves promotes contamination of drinking water and facilitates outbreaks of cryptosporidiosis in humans. Here we report on the Cryptosporidium species and GP60 subtypes of 45 calves originating from the Humid Pampa, the main productive dairy farming area of Argentina. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene was done to determine the infecting Cryptosporidium species and only Cryptosporidium parvum was detected. Subtyping by sequence analysis of the GP60 gene revealed 6 different alleles all pertaining to the zoonotic IIa family. Of these, IIaA23G1R1 represents a novel IIa subtype. Other identified subtypes, IIa18G1R1, IIaA20G1R1, IIaA21G1R1, and IIaA22G1R1 have been recognized in very few studies and/or with low frequencies. Interestingly, different alleles prevailed in the provinces of Buenos Aires (IIaA17G1R1 and IIaA21G1R1), Santa Fe (IIaA23G1R1), and Cordoba (IIaA20G1R1 and IIaA21G1R1), and different allele distribution patterns were observed. Subtypes IIaA18G1R1 and IIaA17G1R1, the latter often found in this study, are strongly implicated in zoonotic transmission, suggesting that calves may represent a potential source for human cryptosporidiosis in this region. This is the first published report of a molecular analysis of Cryptosporidium infection in dairy and beef calves from Argentina.
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maidana, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Dominguez, Mariana Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Veterinary parasitology 198 (3–4) : 382-386. (December 2013)
Materia
Cryptosporidium
Ternero
Calves
Isolation Techniques
Genetics
Técnicas de Aislamiento
Genética
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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Acceder
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spelling Molecular characterization of Cryptosporidium isolates from calves in ArgentinaTomazic, Mariela LujánMaidana, JimenaDominguez, Mariana GabrielaLouge Uriarte, Enrique LeopoldoGalarza, Roxana IvonGarro, Carlos JavierFlorin-Christensen, MonicaSchnittger, LeonhardCryptosporidiumTerneroCalvesIsolation TechniquesGeneticsTécnicas de AislamientoGenéticaCryptosporidiosis is responsible for significant fatalities of neonatal calves, resulting in substantial economic loss in dairy farming in several countries. Additionally, the high shedding of environmentally resistant oocysts by calves promotes contamination of drinking water and facilitates outbreaks of cryptosporidiosis in humans. Here we report on the Cryptosporidium species and GP60 subtypes of 45 calves originating from the Humid Pampa, the main productive dairy farming area of Argentina. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene was done to determine the infecting Cryptosporidium species and only Cryptosporidium parvum was detected. Subtyping by sequence analysis of the GP60 gene revealed 6 different alleles all pertaining to the zoonotic IIa family. Of these, IIaA23G1R1 represents a novel IIa subtype. Other identified subtypes, IIa18G1R1, IIaA20G1R1, IIaA21G1R1, and IIaA22G1R1 have been recognized in very few studies and/or with low frequencies. Interestingly, different alleles prevailed in the provinces of Buenos Aires (IIaA17G1R1 and IIaA21G1R1), Santa Fe (IIaA23G1R1), and Cordoba (IIaA20G1R1 and IIaA21G1R1), and different allele distribution patterns were observed. Subtypes IIaA18G1R1 and IIaA17G1R1, the latter often found in this study, are strongly implicated in zoonotic transmission, suggesting that calves may represent a potential source for human cryptosporidiosis in this region. This is the first published report of a molecular analysis of Cryptosporidium infection in dairy and beef calves from Argentina.Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maidana, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Dominguez, Mariana Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-11-13T17:30:35Z2017-11-13T17:30:35Z2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1757https://www.sciencedirect.com/science/article/pii/S03044017130051650304-4017https://doi.org/10.1016/j.vetpar.2013.09.022Veterinary parasitology 198 (3–4) : 382-386. (December 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-10-16T09:29:04Zoai:localhost:20.500.12123/1757instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:04.654INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of Cryptosporidium isolates from calves in Argentina
title Molecular characterization of Cryptosporidium isolates from calves in Argentina
spellingShingle Molecular characterization of Cryptosporidium isolates from calves in Argentina
Tomazic, Mariela Luján
Cryptosporidium
Ternero
Calves
Isolation Techniques
Genetics
Técnicas de Aislamiento
Genética
title_short Molecular characterization of Cryptosporidium isolates from calves in Argentina
title_full Molecular characterization of Cryptosporidium isolates from calves in Argentina
title_fullStr Molecular characterization of Cryptosporidium isolates from calves in Argentina
title_full_unstemmed Molecular characterization of Cryptosporidium isolates from calves in Argentina
title_sort Molecular characterization of Cryptosporidium isolates from calves in Argentina
dc.creator.none.fl_str_mv Tomazic, Mariela Luján
Maidana, Jimena
Dominguez, Mariana Gabriela
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Garro, Carlos Javier
Florin-Christensen, Monica
Schnittger, Leonhard
author Tomazic, Mariela Luján
author_facet Tomazic, Mariela Luján
Maidana, Jimena
Dominguez, Mariana Gabriela
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Garro, Carlos Javier
Florin-Christensen, Monica
Schnittger, Leonhard
author_role author
author2 Maidana, Jimena
Dominguez, Mariana Gabriela
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Garro, Carlos Javier
Florin-Christensen, Monica
Schnittger, Leonhard
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryptosporidium
Ternero
Calves
Isolation Techniques
Genetics
Técnicas de Aislamiento
Genética
topic Cryptosporidium
Ternero
Calves
Isolation Techniques
Genetics
Técnicas de Aislamiento
Genética
dc.description.none.fl_txt_mv Cryptosporidiosis is responsible for significant fatalities of neonatal calves, resulting in substantial economic loss in dairy farming in several countries. Additionally, the high shedding of environmentally resistant oocysts by calves promotes contamination of drinking water and facilitates outbreaks of cryptosporidiosis in humans. Here we report on the Cryptosporidium species and GP60 subtypes of 45 calves originating from the Humid Pampa, the main productive dairy farming area of Argentina. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene was done to determine the infecting Cryptosporidium species and only Cryptosporidium parvum was detected. Subtyping by sequence analysis of the GP60 gene revealed 6 different alleles all pertaining to the zoonotic IIa family. Of these, IIaA23G1R1 represents a novel IIa subtype. Other identified subtypes, IIa18G1R1, IIaA20G1R1, IIaA21G1R1, and IIaA22G1R1 have been recognized in very few studies and/or with low frequencies. Interestingly, different alleles prevailed in the provinces of Buenos Aires (IIaA17G1R1 and IIaA21G1R1), Santa Fe (IIaA23G1R1), and Cordoba (IIaA20G1R1 and IIaA21G1R1), and different allele distribution patterns were observed. Subtypes IIaA18G1R1 and IIaA17G1R1, the latter often found in this study, are strongly implicated in zoonotic transmission, suggesting that calves may represent a potential source for human cryptosporidiosis in this region. This is the first published report of a molecular analysis of Cryptosporidium infection in dairy and beef calves from Argentina.
Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maidana, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Dominguez, Mariana Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Cryptosporidiosis is responsible for significant fatalities of neonatal calves, resulting in substantial economic loss in dairy farming in several countries. Additionally, the high shedding of environmentally resistant oocysts by calves promotes contamination of drinking water and facilitates outbreaks of cryptosporidiosis in humans. Here we report on the Cryptosporidium species and GP60 subtypes of 45 calves originating from the Humid Pampa, the main productive dairy farming area of Argentina. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene was done to determine the infecting Cryptosporidium species and only Cryptosporidium parvum was detected. Subtyping by sequence analysis of the GP60 gene revealed 6 different alleles all pertaining to the zoonotic IIa family. Of these, IIaA23G1R1 represents a novel IIa subtype. Other identified subtypes, IIa18G1R1, IIaA20G1R1, IIaA21G1R1, and IIaA22G1R1 have been recognized in very few studies and/or with low frequencies. Interestingly, different alleles prevailed in the provinces of Buenos Aires (IIaA17G1R1 and IIaA21G1R1), Santa Fe (IIaA23G1R1), and Cordoba (IIaA20G1R1 and IIaA21G1R1), and different allele distribution patterns were observed. Subtypes IIaA18G1R1 and IIaA17G1R1, the latter often found in this study, are strongly implicated in zoonotic transmission, suggesting that calves may represent a potential source for human cryptosporidiosis in this region. This is the first published report of a molecular analysis of Cryptosporidium infection in dairy and beef calves from Argentina.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
2017-11-13T17:30:35Z
2017-11-13T17:30:35Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1757
https://www.sciencedirect.com/science/article/pii/S0304401713005165
0304-4017
https://doi.org/10.1016/j.vetpar.2013.09.022
url http://hdl.handle.net/20.500.12123/1757
https://www.sciencedirect.com/science/article/pii/S0304401713005165
https://doi.org/10.1016/j.vetpar.2013.09.022
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Veterinary parasitology 198 (3–4) : 382-386. (December 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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