Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.]
- Autores
- Bonafede, Marcos; Tranquilli, Gabriela; Pfluger, Laura Alicia; Peña, Roberto Javier; Dubcovsky, Jorge
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed.
Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Pfluger, Laura Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Peña, Roberto Javier. Centro Internacional de Mejoramiento de Maíz y Trigo; México
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos - Fuente
- Journal of cereal science 62 : 143-150. (March 2015)
- Materia
-
Trigo
Triticum Aestivum
Peso Molecular
Gluteninas
Variación Genética
Alelos
Panificación
Molecular Weight
Glutenins
Genetic Variation
Alleles
Breadmaking
Glu-3/Gli-1 - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1201
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Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.]Bonafede, MarcosTranquilli, GabrielaPfluger, Laura AliciaPeña, Roberto JavierDubcovsky, JorgeTrigoTriticum AestivumPeso MolecularGluteninasVariación GenéticaAlelosPanificaciónMolecular WeightGluteninsGenetic VariationAllelesBreadmakingGlu-3/Gli-1Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed.Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Pfluger, Laura Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Peña, Roberto Javier. Centro Internacional de Mejoramiento de Maíz y Trigo; MéxicoFil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos2017-09-12T17:08:11Z2017-09-12T17:08:11Z2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1201http://www.sciencedirect.com/science/article/pii/S07335210150002110733-5210Journal of cereal science 62 : 143-150. (March 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:09Zoai:localhost:20.500.12123/1201instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.223INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
title |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
spellingShingle |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] Bonafede, Marcos Trigo Triticum Aestivum Peso Molecular Gluteninas Variación Genética Alelos Panificación Molecular Weight Glutenins Genetic Variation Alleles Breadmaking Glu-3/Gli-1 |
title_short |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
title_full |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
title_fullStr |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
title_full_unstemmed |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
title_sort |
Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
dc.creator.none.fl_str_mv |
Bonafede, Marcos Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge |
author |
Bonafede, Marcos |
author_facet |
Bonafede, Marcos Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge |
author_role |
author |
author2 |
Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Trigo Triticum Aestivum Peso Molecular Gluteninas Variación Genética Alelos Panificación Molecular Weight Glutenins Genetic Variation Alleles Breadmaking Glu-3/Gli-1 |
topic |
Trigo Triticum Aestivum Peso Molecular Gluteninas Variación Genética Alelos Panificación Molecular Weight Glutenins Genetic Variation Alleles Breadmaking Glu-3/Gli-1 |
dc.description.none.fl_txt_mv |
Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed. Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Pfluger, Laura Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Peña, Roberto Javier. Centro Internacional de Mejoramiento de Maíz y Trigo; México Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos |
description |
Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03 2017-09-12T17:08:11Z 2017-09-12T17:08:11Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1201 http://www.sciencedirect.com/science/article/pii/S0733521015000211 0733-5210 |
url |
http://hdl.handle.net/20.500.12123/1201 http://www.sciencedirect.com/science/article/pii/S0733521015000211 |
identifier_str_mv |
0733-5210 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Journal of cereal science 62 : 143-150. (March 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619116773638144 |
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12.559606 |