Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)

Autores
Lombardo, Lucio; Nisi, Marí­a Mercedes; Salines, Nicolas; Ghione, Celina Elena; Helguera, Marcelo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be used for the discovery of valuable mutants for basic research and breeding purposes. In this study, we report the identification of 27 HMW-GS M3 mutants based on SDS-PAGE patterns from an EMS mutagenized population of the cultivar Baguette Premium 11. Nine mutations were detected in Ax2*, five in Bx7, four in By8, six in Dx5 and three in Dy10 subunit. Two Ax2* null mutants were characterized at molecular level finding in both cases premature stop codons associated. EMS would tend to generate more premature stop codons in glutenins genes than in others because these have a high frequency of glutamine codons. This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed.
EEA Marcos Juárez
Fil: Lombardo, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina
Fil: Nisi, María Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina
Fil: Salines, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Área Mejoramiento Genético Vegetal; Argentina
Fil: Ghione, Celina Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina
Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina
Fuente
Cytology and genetics 51 (4) : 305–314. (July 2017)
Materia
Trigo
Variedades
Gluteninas
Peso Molecular
Wheat
Varieties
Glutenins
Molecular Weight
Trigo Pan
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2265
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2265
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network_name_str INTA Digital (INTA)
spelling Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)Lombardo, LucioNisi, Marí­a MercedesSalines, NicolasGhione, Celina ElenaHelguera, MarceloTrigoVariedadesGluteninasPeso MolecularWheatVarietiesGluteninsMolecular WeightTrigo PanBread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be used for the discovery of valuable mutants for basic research and breeding purposes. In this study, we report the identification of 27 HMW-GS M3 mutants based on SDS-PAGE patterns from an EMS mutagenized population of the cultivar Baguette Premium 11. Nine mutations were detected in Ax2*, five in Bx7, four in By8, six in Dx5 and three in Dy10 subunit. Two Ax2* null mutants were characterized at molecular level finding in both cases premature stop codons associated. EMS would tend to generate more premature stop codons in glutenins genes than in others because these have a high frequency of glutamine codons. This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed.EEA Marcos JuárezFil: Lombardo, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; ArgentinaFil: Nisi, María Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; ArgentinaFil: Salines, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Área Mejoramiento Genético Vegetal; ArgentinaFil: Ghione, Celina Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; ArgentinaFil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina2018-04-17T18:01:32Z2018-04-17T18:01:32Z2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.3103/S0095452717040028http://hdl.handle.net/20.500.12123/22650095-45271934-9440https://doi.org/10.3103/S0095452717040028Cytology and genetics 51 (4) : 305–314. (July 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:09Zoai:localhost:20.500.12123/2265instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:09.712INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
title Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
spellingShingle Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
Lombardo, Lucio
Trigo
Variedades
Gluteninas
Peso Molecular
Wheat
Varieties
Glutenins
Molecular Weight
Trigo Pan
title_short Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
title_full Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
title_fullStr Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
title_full_unstemmed Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
title_sort Identification of novel high molecular weight glutenin subunit mutants in bread wheat (Triticum aestivum L.)
dc.creator.none.fl_str_mv Lombardo, Lucio
Nisi, Marí­a Mercedes
Salines, Nicolas
Ghione, Celina Elena
Helguera, Marcelo
author Lombardo, Lucio
author_facet Lombardo, Lucio
Nisi, Marí­a Mercedes
Salines, Nicolas
Ghione, Celina Elena
Helguera, Marcelo
author_role author
author2 Nisi, Marí­a Mercedes
Salines, Nicolas
Ghione, Celina Elena
Helguera, Marcelo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Trigo
Variedades
Gluteninas
Peso Molecular
Wheat
Varieties
Glutenins
Molecular Weight
Trigo Pan
topic Trigo
Variedades
Gluteninas
Peso Molecular
Wheat
Varieties
Glutenins
Molecular Weight
Trigo Pan
dc.description.none.fl_txt_mv Bread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be used for the discovery of valuable mutants for basic research and breeding purposes. In this study, we report the identification of 27 HMW-GS M3 mutants based on SDS-PAGE patterns from an EMS mutagenized population of the cultivar Baguette Premium 11. Nine mutations were detected in Ax2*, five in Bx7, four in By8, six in Dx5 and three in Dy10 subunit. Two Ax2* null mutants were characterized at molecular level finding in both cases premature stop codons associated. EMS would tend to generate more premature stop codons in glutenins genes than in others because these have a high frequency of glutamine codons. This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed.
EEA Marcos Juárez
Fil: Lombardo, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina
Fil: Nisi, María Mercedes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina
Fil: Salines, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Área Mejoramiento Genético Vegetal; Argentina
Fil: Ghione, Celina Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina
Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnologia y Recursos Genéticos; Argentina
description Bread wheat (Triticum aestivum L.) is a staple food crop eaten in different ways like pan and other food products. High molecular weight glutenin subunits (HMW-GS) are major determinants of the different wheat end-use qualities. Ethyl-methanesulfonate (EMS) mutagenized populations in plants can be used for the discovery of valuable mutants for basic research and breeding purposes. In this study, we report the identification of 27 HMW-GS M3 mutants based on SDS-PAGE patterns from an EMS mutagenized population of the cultivar Baguette Premium 11. Nine mutations were detected in Ax2*, five in Bx7, four in By8, six in Dx5 and three in Dy10 subunit. Two Ax2* null mutants were characterized at molecular level finding in both cases premature stop codons associated. EMS would tend to generate more premature stop codons in glutenins genes than in others because these have a high frequency of glutamine codons. This type of mutation generates null alleles, therefore they are easily detectable by a low cost protocol like SDS-PAGE. The potential use of knock-out (null alleles) and SDS-PAGE size altered mutants for HMW-GS in wheat quality and nutrition is discussed.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
2018-04-17T18:01:32Z
2018-04-17T18:01:32Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.3103/S0095452717040028
http://hdl.handle.net/20.500.12123/2265
0095-4527
1934-9440
https://doi.org/10.3103/S0095452717040028
url https://link.springer.com/article/10.3103/S0095452717040028
http://hdl.handle.net/20.500.12123/2265
https://doi.org/10.3103/S0095452717040028
identifier_str_mv 0095-4527
1934-9440
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Cytology and genetics 51 (4) : 305–314. (July 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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