Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus

Autores
Niz, José María; Salvador, Ricardo; Ferrelli, María Leticia; Sciocco de Cap, Alicia; Romanowski, Víctor; Berretta, Marcelo Facundo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae.
Instituto de Biotecnología y Biología Molecular
Materia
Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140243
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140243
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple NucleopolyhedrovirusNiz, José MaríaSalvador, RicardoFerrelli, María LeticiaSciocco de Cap, AliciaRomanowski, VíctorBerretta, Marcelo FacundoCiencias ExactasBiologíaFall armywormSfMNPV isolatesBiological controlBaculovirusAgricultural pestThe fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae.Instituto de Biotecnología y Biología Molecular2020-02-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf401-405http://sedici.unlp.edu.ar/handle/10915/140243enginfo:eu-repo/semantics/altIdentifier/issn/1572-994xinfo:eu-repo/semantics/altIdentifier/issn/0920-8569info:eu-repo/semantics/altIdentifier/doi/10.1007/s11262-020-01741-9info:eu-repo/semantics/altIdentifier/pmid/32030574info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:23:59Zoai:sedici.unlp.edu.ar:10915/140243Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:23:59.331SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
spellingShingle Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
Niz, José María
Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
title_short Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_full Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_fullStr Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_full_unstemmed Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
title_sort Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
dc.creator.none.fl_str_mv Niz, José María
Salvador, Ricardo
Ferrelli, María Leticia
Sciocco de Cap, Alicia
Romanowski, Víctor
Berretta, Marcelo Facundo
author Niz, José María
author_facet Niz, José María
Salvador, Ricardo
Ferrelli, María Leticia
Sciocco de Cap, Alicia
Romanowski, Víctor
Berretta, Marcelo Facundo
author_role author
author2 Salvador, Ricardo
Ferrelli, María Leticia
Sciocco de Cap, Alicia
Romanowski, Víctor
Berretta, Marcelo Facundo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
topic Ciencias Exactas
Biología
Fall armyworm
SfMNPV isolates
Biological control
Baculovirus
Agricultural pest
dc.description.none.fl_txt_mv The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae.
Instituto de Biotecnología y Biología Molecular
description The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140243
url http://sedici.unlp.edu.ar/handle/10915/140243
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1572-994x
info:eu-repo/semantics/altIdentifier/issn/0920-8569
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11262-020-01741-9
info:eu-repo/semantics/altIdentifier/pmid/32030574
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
401-405
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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