Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants

Autores
Perez Aguirreburialde, Maria Sol; Gomez, Maria Cristina; Ostachuck, Agustín; Wolman, Federico Javier; Albanesi, Guillermo Luis; Pecora, Andrea; Odeon, Anselmo Carlos; Ardila, Fernando Jorge; Escribano, José M.; Dus Santos, Maria Jose; Wigdorovitz, Andres
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection.
Instituto de Virología
Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ostachuck, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wolman, Federico Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina.
Fil: Albanesi, Guillermo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ardila, Fernando Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Escribano, José M. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Biotecnología; España
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Veterinary Immunology and Immunopathology 151 (3–4) : 315-324. (February 2013)
Materia
Ganado Bovino
Enfermedades de los Animales
Vacuna
Diarrea Viral Bovina
Medicago Sativa
Plantas Transgénicas
Cattle
Animal Diseases
Vaccines
Bovine Viral Diarrhoea
Transgenic Plants
Alfalfa
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4317
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4317
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plantsPerez Aguirreburialde, Maria SolGomez, Maria CristinaOstachuck, AgustínWolman, Federico JavierAlbanesi, Guillermo LuisPecora, AndreaOdeon, Anselmo CarlosArdila, Fernando JorgeEscribano, José M.Dus Santos, Maria JoseWigdorovitz, AndresGanado BovinoEnfermedades de los AnimalesVacunaDiarrea Viral BovinaMedicago SativaPlantas TransgénicasCattleAnimal DiseasesVaccinesBovine Viral DiarrhoeaTransgenic PlantsAlfalfaBovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection.Instituto de VirologíaFil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Ostachuck, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Wolman, Federico Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina.Fil: Albanesi, Guillermo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Ardila, Fernando Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Escribano, José M. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Biotecnología; EspañaFil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaElsevier2019-01-23T14:27:20Z2019-01-23T14:27:20Z2013-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0165242712004291http://hdl.handle.net/20.500.12123/43170165-2427https://doi.org/10.1016/j.vetimm.2012.12.004Veterinary Immunology and Immunopathology 151 (3–4) : 315-324. (February 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4317instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.08INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
title Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
spellingShingle Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
Perez Aguirreburialde, Maria Sol
Ganado Bovino
Enfermedades de los Animales
Vacuna
Diarrea Viral Bovina
Medicago Sativa
Plantas Transgénicas
Cattle
Animal Diseases
Vaccines
Bovine Viral Diarrhoea
Transgenic Plants
Alfalfa
title_short Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
title_full Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
title_fullStr Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
title_full_unstemmed Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
title_sort Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants
dc.creator.none.fl_str_mv Perez Aguirreburialde, Maria Sol
Gomez, Maria Cristina
Ostachuck, Agustín
Wolman, Federico Javier
Albanesi, Guillermo Luis
Pecora, Andrea
Odeon, Anselmo Carlos
Ardila, Fernando Jorge
Escribano, José M.
Dus Santos, Maria Jose
Wigdorovitz, Andres
author Perez Aguirreburialde, Maria Sol
author_facet Perez Aguirreburialde, Maria Sol
Gomez, Maria Cristina
Ostachuck, Agustín
Wolman, Federico Javier
Albanesi, Guillermo Luis
Pecora, Andrea
Odeon, Anselmo Carlos
Ardila, Fernando Jorge
Escribano, José M.
Dus Santos, Maria Jose
Wigdorovitz, Andres
author_role author
author2 Gomez, Maria Cristina
Ostachuck, Agustín
Wolman, Federico Javier
Albanesi, Guillermo Luis
Pecora, Andrea
Odeon, Anselmo Carlos
Ardila, Fernando Jorge
Escribano, José M.
Dus Santos, Maria Jose
Wigdorovitz, Andres
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Vacuna
Diarrea Viral Bovina
Medicago Sativa
Plantas Transgénicas
Cattle
Animal Diseases
Vaccines
Bovine Viral Diarrhoea
Transgenic Plants
Alfalfa
topic Ganado Bovino
Enfermedades de los Animales
Vacuna
Diarrea Viral Bovina
Medicago Sativa
Plantas Transgénicas
Cattle
Animal Diseases
Vaccines
Bovine Viral Diarrhoea
Transgenic Plants
Alfalfa
dc.description.none.fl_txt_mv Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection.
Instituto de Virología
Fil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Ostachuck, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wolman, Federico Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina.
Fil: Albanesi, Guillermo Luis. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Odeon, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ardila, Fernando Jorge. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Escribano, José M. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Departamento de Biotecnología; España
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wigdorovitz, Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-15
2019-01-23T14:27:20Z
2019-01-23T14:27:20Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0165242712004291
http://hdl.handle.net/20.500.12123/4317
0165-2427
https://doi.org/10.1016/j.vetimm.2012.12.004
url https://www.sciencedirect.com/science/article/pii/S0165242712004291
http://hdl.handle.net/20.500.12123/4317
https://doi.org/10.1016/j.vetimm.2012.12.004
identifier_str_mv 0165-2427
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 151 (3–4) : 315-324. (February 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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