Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software

Autores
Ledesma, Alba Verónica; Zalazar, Lucía; Greco, Micaela; Hozbor, Federico Andres; Cesari, Andreina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
EEA Balcarce
Fil: Ledesma, Alba Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ledesma, Alba Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zalazar, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zalazar, Lucía. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Greco, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Greco, Micaela. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cesari, Andreina. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fuente
Tropical Animal Health and Production 53 : 499 (2021)
Materia
Proteínas Recombinantes
Morueco
Espermatozoo
Criopreservación
Recombinant Proteins
Rams
Spermatozoa
Cryopreservation
TrxAFNIIx4His6
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25488
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25488
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker softwareLedesma, Alba VerónicaZalazar, LucíaGreco, MicaelaHozbor, Federico AndresCesari, AndreinaProteínas RecombinantesMoruecoEspermatozooCriopreservaciónRecombinant ProteinsRamsSpermatozoaCryopreservationTrxAFNIIx4His6The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.EEA BalcarceFil: Ledesma, Alba Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Ledesma, Alba Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Zalazar, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Zalazar, Lucía. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Greco, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Greco, Micaela. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Cesari, Andreina. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaSpringer2026-03-17T13:36:36Z2026-03-17T13:36:36Z2021-10-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25488https://link.springer.com/article/10.1007/s11250-021-02954-21573-7438 (en línea)0049-4747 (impreso)https://doi.org/10.1007/s11250-021-02954-2Tropical Animal Health and Production 53 : 499 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-03-26T11:25:30Zoai:localhost:20.500.12123/25488instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-03-26 11:25:31.154INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
title Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
spellingShingle Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
Ledesma, Alba Verónica
Proteínas Recombinantes
Morueco
Espermatozoo
Criopreservación
Recombinant Proteins
Rams
Spermatozoa
Cryopreservation
TrxAFNIIx4His6
title_short Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
title_full Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
title_fullStr Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
title_full_unstemmed Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
title_sort Recombinant TrxAFNIIx4His6 improves post‑thaw motility of ram sperm measured by a sperm motility tracker software
dc.creator.none.fl_str_mv Ledesma, Alba Verónica
Zalazar, Lucía
Greco, Micaela
Hozbor, Federico Andres
Cesari, Andreina
author Ledesma, Alba Verónica
author_facet Ledesma, Alba Verónica
Zalazar, Lucía
Greco, Micaela
Hozbor, Federico Andres
Cesari, Andreina
author_role author
author2 Zalazar, Lucía
Greco, Micaela
Hozbor, Federico Andres
Cesari, Andreina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Proteínas Recombinantes
Morueco
Espermatozoo
Criopreservación
Recombinant Proteins
Rams
Spermatozoa
Cryopreservation
TrxAFNIIx4His6
topic Proteínas Recombinantes
Morueco
Espermatozoo
Criopreservación
Recombinant Proteins
Rams
Spermatozoa
Cryopreservation
TrxAFNIIx4His6
dc.description.none.fl_txt_mv The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
EEA Balcarce
Fil: Ledesma, Alba Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Ledesma, Alba Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zalazar, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zalazar, Lucía. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Greco, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Greco, Micaela. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cesari, Andreina. Universidad Nacional de Mar del Plata (UNMdP). Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-06
2026-03-17T13:36:36Z
2026-03-17T13:36:36Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25488
https://link.springer.com/article/10.1007/s11250-021-02954-2
1573-7438 (en línea)
0049-4747 (impreso)
https://doi.org/10.1007/s11250-021-02954-2
url http://hdl.handle.net/20.500.12123/25488
https://link.springer.com/article/10.1007/s11250-021-02954-2
https://doi.org/10.1007/s11250-021-02954-2
identifier_str_mv 1573-7438 (en línea)
0049-4747 (impreso)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Tropical Animal Health and Production 53 : 499 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.977003

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