Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software

Autores
Ledesma, Alba; Zalazar, Lucia; Greco, Micaela Belen; Hozbor, Federico Andrés; Cesari, Andreina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
Fil: Ledesma, Alba. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zalazar, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Greco, Micaela Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
RAM
RECOMBINANT PROTEINS
SEMEN EXTENDER
SPERM SUBPOPULATIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149219
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/149219
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker softwareLedesma, AlbaZalazar, LuciaGreco, Micaela BelenHozbor, Federico AndrésCesari, AndreinaRAMRECOMBINANT PROTEINSSEMEN EXTENDERSPERM SUBPOPULATIONShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.Fil: Ledesma, Alba. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zalazar, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Greco, Micaela Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; ArgentinaFil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaSpringer2021-10-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149219Ledesma, Alba; Zalazar, Lucia; Greco, Micaela Belen; Hozbor, Federico Andrés; Cesari, Andreina; Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software; Springer; Tropical Animal Health And Production; 53; 5; 6-10-2021; 1-60049-4747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11250-021-02954-2info:eu-repo/semantics/altIdentifier/doi/10.1007/s11250-021-02954-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:27:46Zoai:ri.conicet.gov.ar:11336/149219instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:27:47.123CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
title Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
spellingShingle Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
Ledesma, Alba
RAM
RECOMBINANT PROTEINS
SEMEN EXTENDER
SPERM SUBPOPULATIONS
title_short Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
title_full Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
title_fullStr Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
title_full_unstemmed Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
title_sort Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software
dc.creator.none.fl_str_mv Ledesma, Alba
Zalazar, Lucia
Greco, Micaela Belen
Hozbor, Federico Andrés
Cesari, Andreina
author Ledesma, Alba
author_facet Ledesma, Alba
Zalazar, Lucia
Greco, Micaela Belen
Hozbor, Federico Andrés
Cesari, Andreina
author_role author
author2 Zalazar, Lucia
Greco, Micaela Belen
Hozbor, Federico Andrés
Cesari, Andreina
author2_role author
author
author
author
dc.subject.none.fl_str_mv RAM
RECOMBINANT PROTEINS
SEMEN EXTENDER
SPERM SUBPOPULATIONS
topic RAM
RECOMBINANT PROTEINS
SEMEN EXTENDER
SPERM SUBPOPULATIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
Fil: Ledesma, Alba. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zalazar, Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Greco, Micaela Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Hozbor, Federico Andrés. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Laboratorio de Biotecnología de la Reproduccion; Argentina
Fil: Cesari, Andreina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description The aim of the present study was to evaluate a freezing extender supplemented with recombinant TrxAFNIIx4His6, a reported decapacitating factor. Semen samples were diluted in tris-egg yolk medium with 0, 1.5 µM and 3.0 µM of TrxAFNIIx4His6. Computer-assisted sperm motility tracking and subpopulations evaluation showed that addition of TrxAFNIIx4His6 improved post-thaw total and progressive motility at both concentrations evaluated. TrxAFNIIx4His6 increased the sperm subpopulation with the highest progressiveness and great velocity and decreased the subpopulation of poorly motile and almost non-progressive sperm. Incorporation of TrxAFNIIx4His6 to freezing extender shows potential for the development of cryoprotection media which may lead to improved fertility after artificial insemination.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149219
Ledesma, Alba; Zalazar, Lucia; Greco, Micaela Belen; Hozbor, Federico Andrés; Cesari, Andreina; Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software; Springer; Tropical Animal Health And Production; 53; 5; 6-10-2021; 1-6
0049-4747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149219
identifier_str_mv Ledesma, Alba; Zalazar, Lucia; Greco, Micaela Belen; Hozbor, Federico Andrés; Cesari, Andreina; Recombinant TrxAFNIIx4His6 improves post-thaw motility of ram sperm measured by a sperm motility tracker software; Springer; Tropical Animal Health And Production; 53; 5; 6-10-2021; 1-6
0049-4747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s11250-021-02954-2
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11250-021-02954-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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