Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice

Autores
Reidel, Ivana Gabriela; Garcia, Maria Ines; González, Verónica Doris Guadalupe; Giorello, Antonella; Calvinho, Luis Fernando; Gennaro, Ana Maria; Veaute, Carolina Melania Isabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses.
EEA Rafaela
Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Giorello, Antonella. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: Gennaro, Ana Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fuente
International Journal For Research In Applied And Natural Science 3 (6) : 1-19 (June 2017)
Materia
Vacuna
Respuesta Inmunológica
Coadyuvantes
Ratón
Liposomas (organulos)
Vaccines
Immune Response
Adjuvants
Mice
Liposomes (organelles)
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5024
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5024
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in miceReidel, Ivana GabrielaGarcia, Maria InesGonzález, Verónica Doris GuadalupeGiorello, AntonellaCalvinho, Luis FernandoGennaro, Ana MariaVeaute, Carolina Melania IsabelVacunaRespuesta InmunológicaCoadyuvantesRatónLiposomas (organulos)VaccinesImmune ResponseAdjuvantsMiceLiposomes (organelles)The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses.EEA RafaelaFil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Giorello, Antonella. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.Fil: Gennaro, Ana Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; ArgentinaFil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.International Journal for Research2019-05-03T13:33:58Z2019-05-03T13:33:58Z2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://gnpublication.org/index.php/ans/article/view/81/73http://hdl.handle.net/20.500.12123/50242208-2085International Journal For Research In Applied And Natural Science 3 (6) : 1-19 (June 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:57Zoai:localhost:20.500.12123/5024instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:57.526INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
title Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
spellingShingle Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
Reidel, Ivana Gabriela
Vacuna
Respuesta Inmunológica
Coadyuvantes
Ratón
Liposomas (organulos)
Vaccines
Immune Response
Adjuvants
Mice
Liposomes (organelles)
title_short Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
title_full Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
title_fullStr Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
title_full_unstemmed Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
title_sort Effects of the liposomal co-encapsulation of antigen and PO-CpG oligonucleotide on immune response in mice
dc.creator.none.fl_str_mv Reidel, Ivana Gabriela
Garcia, Maria Ines
González, Verónica Doris Guadalupe
Giorello, Antonella
Calvinho, Luis Fernando
Gennaro, Ana Maria
Veaute, Carolina Melania Isabel
author Reidel, Ivana Gabriela
author_facet Reidel, Ivana Gabriela
Garcia, Maria Ines
González, Verónica Doris Guadalupe
Giorello, Antonella
Calvinho, Luis Fernando
Gennaro, Ana Maria
Veaute, Carolina Melania Isabel
author_role author
author2 Garcia, Maria Ines
González, Verónica Doris Guadalupe
Giorello, Antonella
Calvinho, Luis Fernando
Gennaro, Ana Maria
Veaute, Carolina Melania Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Vacuna
Respuesta Inmunológica
Coadyuvantes
Ratón
Liposomas (organulos)
Vaccines
Immune Response
Adjuvants
Mice
Liposomes (organelles)
topic Vacuna
Respuesta Inmunológica
Coadyuvantes
Ratón
Liposomas (organulos)
Vaccines
Immune Response
Adjuvants
Mice
Liposomes (organelles)
dc.description.none.fl_txt_mv The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses.
EEA Rafaela
Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: González, Verónica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Giorello, Antonella. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
Fil: Gennaro, Ana Maria. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Física del Litoral. Universidad Nacional del Litoral. Instituto de Física del Litoral; Argentina
Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina.
description The development of novel vaccines requires the design of new adjuvants able to give long lasting immune responses. Our aim was to obtain cationic liposomes as adjuvants by an industry-suitable method, and evaluate them using bovine serum albumin (BSA) as immunogen and CpG oligonucleotides with phosphodiesther bonds, as immunostimulants. Liposomes (Lip) were prepared with dipalmitoylphosphatidylcholine, cholesterol and stearylamine by Ethanol Injection method. Immune response was assessed by immunization of Balb/c mice with: Lip+BSA Lip+BSA+CpG, CpG+BSA or aluminium hydroxide (Al(OH)3+BSA). Liposomal formulations were able to induce high antibody levels. Lip+BSA+CpG led to higher IgG levels than Lip+BSA (p<0.05, Mann-Whitney) and elicited the highest IgG2a levels. All the formulations induced antigen specific cellular proliferation, without significant differences, meanwhile Lip+BSA+CpG produced the highest levels of IFN-γ. These results showed these liposomes are versatile vehicles to potentiate and target the immune system to vaccination, leading to high humoral and cellular immune responses.
publishDate 2017
dc.date.none.fl_str_mv 2017-06
2019-05-03T13:33:58Z
2019-05-03T13:33:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://gnpublication.org/index.php/ans/article/view/81/73
http://hdl.handle.net/20.500.12123/5024
2208-2085
url https://gnpublication.org/index.php/ans/article/view/81/73
http://hdl.handle.net/20.500.12123/5024
identifier_str_mv 2208-2085
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Journal for Research
publisher.none.fl_str_mv International Journal for Research
dc.source.none.fl_str_mv International Journal For Research In Applied And Natural Science 3 (6) : 1-19 (June 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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