The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model

Autores
Puente, Mariana Laura; Gualpa, Jose Luis; López, Gastón A.; Molina, Romina M.; Carletti, Susana; Cassán, Fabricio D.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC−) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC− or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment.
Inst. de Microbiología y Zoología Agrícola IMyZA
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina
Fil: Gualpa, Jose Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fil: López, Gastón A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fil: Molina, Romina M. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fil: Carletti, Susana. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Cassán, Fabricio D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fuente
Symbiosis 76 (1) : 41–49 (September 2018)
Materia
Azospirillum brasilense
Inoculación
Soja
Auxinas
Inoculation
Soybeans
Auxins
Inoculación Foliar
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1911
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1911
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling modelPuente, Mariana LauraGualpa, Jose LuisLópez, Gastón A.Molina, Romina M.Carletti, SusanaCassán, Fabricio D.Azospirillum brasilenseInoculaciónSojaAuxinasInoculationSoybeansAuxinsInoculación FoliarAzospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC−) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC− or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment.Inst. de Microbiología y Zoología Agrícola IMyZAFil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; ArgentinaFil: Gualpa, Jose Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; ArgentinaFil: López, Gastón A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; ArgentinaFil: Molina, Romina M. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; ArgentinaFil: Carletti, Susana. Universidad Nacional de Luján. Departamento de Ciencias Básicas; ArgentinaFil: Cassán, Fabricio D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina2018-02-28T15:24:37Z2018-02-28T15:24:37Z2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1911https://link.springer.com/article/10.1007/s13199-017-0536-x0334-51141878-7665https://doi.org/10.1007/s13199-017-0536-xSymbiosis 76 (1) : 41–49 (September 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:09Zoai:localhost:20.500.12123/1911instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:09.867INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
title The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
spellingShingle The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
Puente, Mariana Laura
Azospirillum brasilense
Inoculación
Soja
Auxinas
Inoculation
Soybeans
Auxins
Inoculación Foliar
title_short The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
title_full The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
title_fullStr The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
title_full_unstemmed The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
title_sort The benefits of foliar inoculation with Azospirillum brasilense in soybean are explained by an auxin signaling model
dc.creator.none.fl_str_mv Puente, Mariana Laura
Gualpa, Jose Luis
López, Gastón A.
Molina, Romina M.
Carletti, Susana
Cassán, Fabricio D.
author Puente, Mariana Laura
author_facet Puente, Mariana Laura
Gualpa, Jose Luis
López, Gastón A.
Molina, Romina M.
Carletti, Susana
Cassán, Fabricio D.
author_role author
author2 Gualpa, Jose Luis
López, Gastón A.
Molina, Romina M.
Carletti, Susana
Cassán, Fabricio D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Azospirillum brasilense
Inoculación
Soja
Auxinas
Inoculation
Soybeans
Auxins
Inoculación Foliar
topic Azospirillum brasilense
Inoculación
Soja
Auxinas
Inoculation
Soybeans
Auxins
Inoculación Foliar
dc.description.none.fl_txt_mv Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC−) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC− or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment.
Inst. de Microbiología y Zoología Agrícola IMyZA
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina
Fil: Gualpa, Jose Luis. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fil: López, Gastón A. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fil: Molina, Romina M. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
Fil: Carletti, Susana. Universidad Nacional de Luján. Departamento de Ciencias Básicas; Argentina
Fil: Cassán, Fabricio D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal y de la Interacción planta-microorganismo; Argentina
description Azospirillum sp. is one of the most studied genera of plant growth-promoting rhizobacteria (PGPR). The ability of Azospirillum sp. to promote plant growth has been associated with its ability to produce several phytohormones, such as auxins, gibberellins and cytokinins, but mainly indole-3-acetic acid (IAA). It has been propoosed that the production of IAA explains the positive effects of co-inoculation with Azospirillum sp. on the rhizobia-legume symbiosis. In this study, we constructed an IAA-deficient mutant of A. brasilense Az39 (ipdC−) by using a restriction-free cloning method. We inoculated soybean seeds with 1·106 cfu·seed−1 of Bradyrhizobium japonicum E109 and co-inoculating leaves at the V3 stage with 1·108 cfu.plant−1 of A. brasilense Az39 wt or ipdC− or inoculated leaves with 20 μg.plant−1 synthetic IAA. The results confirmed soybean growth promotion as there was increased total plant and root length, aerial and root dry weight, number of nodules on the primary root, and an increase in the symbiosis established with B. japonicum E109. Nodule weight also increased after foliar co-inoculation with the IAA- producer A. brasilense Az39. The exogenous application of IAA decreased aerial and root length, as well as the number of nodules on primary roots in comparison with the Az39 wt strain. These results allow us to propose a biological model of response to foliar co-inoculation of soybean with IAA-producing rhizobacteria. This model clearly shows that both the presence of microorganism as part of the colonization process and the production of IAA in situ are co-responsible, via plant signaling molecules, for the positive effects on plant growth and symbiosis establishment.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-28T15:24:37Z
2018-02-28T15:24:37Z
2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1911
https://link.springer.com/article/10.1007/s13199-017-0536-x
0334-5114
1878-7665
https://doi.org/10.1007/s13199-017-0536-x
url http://hdl.handle.net/20.500.12123/1911
https://link.springer.com/article/10.1007/s13199-017-0536-x
https://doi.org/10.1007/s13199-017-0536-x
identifier_str_mv 0334-5114
1878-7665
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Symbiosis 76 (1) : 41–49 (September 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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