Localization and survival of Azospirillum brasilense Az39 in soybean leaves

Autores
Puente, Mariana Laura; Maroniche, Guillermo Andrés; Panepucci, Marina Gabriela; Sabio Y Garcia, Julia Veronica; García, Julia Elena; Criado, María Victoria; Molina, Romina Micaela; Cassán, Fabricio Dario
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Panepucci, Marina Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Criado, Maria Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA)-CONICET; Argentina
Fil: Molina, Romina Micaela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentina
Fil: Cassán, Fabricio Darío. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentina
Fuente
Letters in Applied Microbiology 72 (5) : 626-633 (May 2021)
Materia
Azospirillum brasilense
Inoculation
Soybeans
Soja
Inoculación
PGPR
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10996
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10996
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network_name_str INTA Digital (INTA)
spelling Localization and survival of Azospirillum brasilense Az39 in soybean leavesPuente, Mariana LauraMaroniche, Guillermo AndrésPanepucci, Marina GabrielaSabio Y Garcia, Julia VeronicaGarcía, Julia ElenaCriado, María VictoriaMolina, Romina MicaelaCassán, Fabricio DarioAzospirillum brasilenseInoculationSoybeansSojaInoculaciónPGPRIn recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Panepucci, Marina Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; ArgentinaFil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Criado, Maria Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA)-CONICET; ArgentinaFil: Molina, Romina Micaela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; ArgentinaFil: Cassán, Fabricio Darío. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; ArgentinaWiley2021-12-27T16:49:22Z2021-12-27T16:49:22Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10996https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.134440266-8254https://doi.org/10.1111/lam.13444Letters in Applied Microbiology 72 (5) : 626-633 (May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:14Zoai:localhost:20.500.12123/10996instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:14.349INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Localization and survival of Azospirillum brasilense Az39 in soybean leaves
title Localization and survival of Azospirillum brasilense Az39 in soybean leaves
spellingShingle Localization and survival of Azospirillum brasilense Az39 in soybean leaves
Puente, Mariana Laura
Azospirillum brasilense
Inoculation
Soybeans
Soja
Inoculación
PGPR
title_short Localization and survival of Azospirillum brasilense Az39 in soybean leaves
title_full Localization and survival of Azospirillum brasilense Az39 in soybean leaves
title_fullStr Localization and survival of Azospirillum brasilense Az39 in soybean leaves
title_full_unstemmed Localization and survival of Azospirillum brasilense Az39 in soybean leaves
title_sort Localization and survival of Azospirillum brasilense Az39 in soybean leaves
dc.creator.none.fl_str_mv Puente, Mariana Laura
Maroniche, Guillermo Andrés
Panepucci, Marina Gabriela
Sabio Y Garcia, Julia Veronica
García, Julia Elena
Criado, María Victoria
Molina, Romina Micaela
Cassán, Fabricio Dario
author Puente, Mariana Laura
author_facet Puente, Mariana Laura
Maroniche, Guillermo Andrés
Panepucci, Marina Gabriela
Sabio Y Garcia, Julia Veronica
García, Julia Elena
Criado, María Victoria
Molina, Romina Micaela
Cassán, Fabricio Dario
author_role author
author2 Maroniche, Guillermo Andrés
Panepucci, Marina Gabriela
Sabio Y Garcia, Julia Veronica
García, Julia Elena
Criado, María Victoria
Molina, Romina Micaela
Cassán, Fabricio Dario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Azospirillum brasilense
Inoculation
Soybeans
Soja
Inoculación
PGPR
topic Azospirillum brasilense
Inoculation
Soybeans
Soja
Inoculación
PGPR
dc.description.none.fl_txt_mv In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Puente, Mariana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Laboratorio de Bacterias Promotoras del Crecimiento Vegetal; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Panepucci, Marina Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Laboratorio Integral de Microscopía; Argentina
Fil: Garcia, Julia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Criado, Maria Victoria. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA)-CONICET; Argentina
Fil: Molina, Romina Micaela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentina
Fil: Cassán, Fabricio Darío. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas. Laboratorio de Fisiología Vegetal e Interacción Planta-Microorganismo. INIAB-CONICET; Argentina
description In recent years, foliar inoculation has gained acceptance among the available methods to deliver plant beneficial micro-organisms to crops under field conditions. Colonization efficiency by such micro-organisms largely depends on their ability to survive when applied on the leaves. In this work, we evaluated the survival and localization of Azospirillum brasilense Az39 (Az39) in excised soybean leaves. Scanning electron microscopy and confocal laser scanning microscopy of a red fluorescent-transformed variant of Az39 were used to determine bacterial localization, while the most probable number and plate count methods were applied for bacterial quantification. Microscopic observations indicated a decrease in the number of Az39 cells on the leaf surface at 24 h after treatment, whereas midribs and cell–cell junctions of the inner leaf epidermis became highly populated zones. The presence of Az39 inside xylem vessels was corroborated at 6 h after bacterization. Az39 population did not significantly decrease throughout 24 h. We could visualize Az39 cells on the surface and in internal tissues of soybean leaves and recover them through culture methodologies. These results evidence the survival capacity of Az39 on and inside leaves and suggest a previously unnoticed endophytic potential for this well-known plant growth-promoting rhizobacteria strain.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-27T16:49:22Z
2021-12-27T16:49:22Z
2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10996
https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13444
0266-8254
https://doi.org/10.1111/lam.13444
url http://hdl.handle.net/20.500.12123/10996
https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/lam.13444
https://doi.org/10.1111/lam.13444
identifier_str_mv 0266-8254
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Letters in Applied Microbiology 72 (5) : 626-633 (May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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