Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita

Autores
Cap, Guillermo Bartolome; Roberts, P.A.; Thomason, I.J.; Murashige, T.
Año de publicación
1991
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptible cultivars UC82, Lukullus, Tropic, and male-sterile line ms-31, respectively. The incongruity barrier between the two plant species was overcome by embryo callus and embryo cloning techniques. Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. These results confirmed the successful transfer and expression of heat-stable resistance to M. incognita from L. peruvianum to hybrids with L. esculentum as a preliminary step to introgressing additional root-knot nematode resistance into tomato.
EEA AMBA
Fil: Cap, Guillermo Bartolome. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; Argentina
Fil: Roberts, P.A. University of California, Riverside. Department of Hematology; Estados Unidos
Fil: Thomason, I.J. University of California, Riverside. Department of Hematology; Estados Unidos
Fil: Murashige, T. University of California, Riverside. Department of Hematology; Estados Unidos
Fuente
Journal of the American Society for Horticultural Science 116 (6) : 1082–1088 (November 1991)
Materia
Tomate
Solanum peruvianum
Híbridos
Cultivo de Embriones
Genotipos
Meloidogyne incognita
Resistencia a la Enfermedad
Tomatoes
Hybrids
Embryo Culture
Genotypes
Disease Resistance
Lycopersicon esculentum
Lycopersicon peruvianum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7184
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network_name_str INTA Digital (INTA)
spelling Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognitaCap, Guillermo BartolomeRoberts, P.A.Thomason, I.J.Murashige, T.TomateSolanum peruvianumHíbridosCultivo de EmbrionesGenotiposMeloidogyne incognitaResistencia a la EnfermedadTomatoesHybridsEmbryo CultureGenotypesDisease ResistanceLycopersicon esculentumLycopersicon peruvianumGenotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptible cultivars UC82, Lukullus, Tropic, and male-sterile line ms-31, respectively. The incongruity barrier between the two plant species was overcome by embryo callus and embryo cloning techniques. Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. These results confirmed the successful transfer and expression of heat-stable resistance to M. incognita from L. peruvianum to hybrids with L. esculentum as a preliminary step to introgressing additional root-knot nematode resistance into tomato.EEA AMBAFil: Cap, Guillermo Bartolome. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; ArgentinaFil: Roberts, P.A. University of California, Riverside. Department of Hematology; Estados UnidosFil: Thomason, I.J. University of California, Riverside. Department of Hematology; Estados UnidosFil: Murashige, T. University of California, Riverside. Department of Hematology; Estados UnidosAmerican Society for Horticultural Science2020-05-04T13:36:49Z2020-05-04T13:36:49Z1991-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://journals.ashs.org/jashs/view/journals/jashs/116/6/article-p1082.xmlhttp://hdl.handle.net/20.500.12123/71840003-10622327-9788https://doi.org/10.21273/JASHS.116.6.1082Journal of the American Society for Horticultural Science 116 (6) : 1082–1088 (November 1991)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:16Zoai:localhost:20.500.12123/7184instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:16.514INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
title Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
spellingShingle Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
Cap, Guillermo Bartolome
Tomate
Solanum peruvianum
Híbridos
Cultivo de Embriones
Genotipos
Meloidogyne incognita
Resistencia a la Enfermedad
Tomatoes
Hybrids
Embryo Culture
Genotypes
Disease Resistance
Lycopersicon esculentum
Lycopersicon peruvianum
title_short Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
title_full Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
title_fullStr Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
title_full_unstemmed Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
title_sort Embryo Culture of Lycopersicon esculentum × L. peruvianum Hybrid Genotypes Possessing Heat-stable Resistance to Meloidogyne incognita
dc.creator.none.fl_str_mv Cap, Guillermo Bartolome
Roberts, P.A.
Thomason, I.J.
Murashige, T.
author Cap, Guillermo Bartolome
author_facet Cap, Guillermo Bartolome
Roberts, P.A.
Thomason, I.J.
Murashige, T.
author_role author
author2 Roberts, P.A.
Thomason, I.J.
Murashige, T.
author2_role author
author
author
dc.subject.none.fl_str_mv Tomate
Solanum peruvianum
Híbridos
Cultivo de Embriones
Genotipos
Meloidogyne incognita
Resistencia a la Enfermedad
Tomatoes
Hybrids
Embryo Culture
Genotypes
Disease Resistance
Lycopersicon esculentum
Lycopersicon peruvianum
topic Tomate
Solanum peruvianum
Híbridos
Cultivo de Embriones
Genotipos
Meloidogyne incognita
Resistencia a la Enfermedad
Tomatoes
Hybrids
Embryo Culture
Genotypes
Disease Resistance
Lycopersicon esculentum
Lycopersicon peruvianum
dc.description.none.fl_txt_mv Genotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptible cultivars UC82, Lukullus, Tropic, and male-sterile line ms-31, respectively. The incongruity barrier between the two plant species was overcome by embryo callus and embryo cloning techniques. Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. These results confirmed the successful transfer and expression of heat-stable resistance to M. incognita from L. peruvianum to hybrids with L. esculentum as a preliminary step to introgressing additional root-knot nematode resistance into tomato.
EEA AMBA
Fil: Cap, Guillermo Bartolome. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Área Metropolitana de Buenos Aires. Agencia de Extensión Rural La Plata; Argentina
Fil: Roberts, P.A. University of California, Riverside. Department of Hematology; Estados Unidos
Fil: Thomason, I.J. University of California, Riverside. Department of Hematology; Estados Unidos
Fil: Murashige, T. University of California, Riverside. Department of Hematology; Estados Unidos
description Genotypes of Lycopersicon peruvianum (L.) Mill. and L. peruvianum var. glandulosum (Rick), selected from accessions that possess resistance to Meloidogyne incognita [(Kofoid and White) Chitwood] at high soil temperature (30C), were used as male parents in crosses with L. esculentum (Mill.) susceptible cultivars UC82, Lukullus, Tropic, and male-sterile line ms-31, respectively. The incongruity barrier between the two plant species was overcome by embryo callus and embryo cloning techniques. Hybridity of the F, progeny obtained from each cross was confirmed by differences in leaf and flower morphology, plant growth habits, and by acid phosphatase isozyme phenotypes using polyacrylamide gel electrophoresis. In greenhouse inoculation experiments, F1 plants were highly resistant to M. incognita in soil at 25 and 30C. These results confirmed the successful transfer and expression of heat-stable resistance to M. incognita from L. peruvianum to hybrids with L. esculentum as a preliminary step to introgressing additional root-knot nematode resistance into tomato.
publishDate 1991
dc.date.none.fl_str_mv 1991-11
2020-05-04T13:36:49Z
2020-05-04T13:36:49Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.ashs.org/jashs/view/journals/jashs/116/6/article-p1082.xml
http://hdl.handle.net/20.500.12123/7184
0003-1062
2327-9788
https://doi.org/10.21273/JASHS.116.6.1082
url https://journals.ashs.org/jashs/view/journals/jashs/116/6/article-p1082.xml
http://hdl.handle.net/20.500.12123/7184
https://doi.org/10.21273/JASHS.116.6.1082
identifier_str_mv 0003-1062
2327-9788
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Horticultural Science
publisher.none.fl_str_mv American Society for Horticultural Science
dc.source.none.fl_str_mv Journal of the American Society for Horticultural Science 116 (6) : 1082–1088 (November 1991)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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