Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h
- Autores
- Aller Atucha, Juan Florencio; Abalos, Marcos; Acuña, Francisco Antonio; Virgili, Rosana; Requena, Francisco; Cancino, Andrea Karina
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20 llamas flushed at Day 7 after mating. Fourteen excellent hatched blastocysts were stored in sterile test tubes containing 1.5 ml of TCM 199-Hepes + 10% cow serum (v/v concentration) + antibiotics and cooled to 5 °C (cooling rate = 0.5 °C/min) and maintained at 5 °C during 24 h. After this, the embryos were warmed on warming plate (38 °C), washed three times and loaded individually into 0.25 ml French straws. Fourteen adult llamas were used as recipients and embryos were transferred only into the left uterine horn of the recipient regardless of side ovulation. All recipient llamas were pregnancy tested by transrectal ultrasonography at 45 days after transfer. Three (21.5%) pregnant females were detected and delivered three normal healthy male calves at term. To our knowledge, these offspring are the first llamas produced following transfer of refrigerated embryos. In summary, llama embryos can be cooled to 5 °C and maintained in storage for up to 24 h without significant losses of viability.
EEA Balcarce
Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Abalos, Marcos. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Acuña, Francisco Antonio. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Virgili, Rosana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; Argentina
Fil: Requena, Francisco. Universidad de Córdoba. Facultad de Veterinaria. Campus Universitario Rabanales; España
Fil: Cancino, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina - Fuente
- Small ruminant research 132 : 99-102. (November 2015)
- Materia
-
Llama
Transferencia de Embriones
Parto
Llamas
Embryo Transfer
Parturition
Nacimiento - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2228
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Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 hAller Atucha, Juan FlorencioAbalos, MarcosAcuña, Francisco AntonioVirgili, RosanaRequena, FranciscoCancino, Andrea KarinaLlamaTransferencia de EmbrionesPartoLlamasEmbryo TransferParturitionNacimientoThe objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20 llamas flushed at Day 7 after mating. Fourteen excellent hatched blastocysts were stored in sterile test tubes containing 1.5 ml of TCM 199-Hepes + 10% cow serum (v/v concentration) + antibiotics and cooled to 5 °C (cooling rate = 0.5 °C/min) and maintained at 5 °C during 24 h. After this, the embryos were warmed on warming plate (38 °C), washed three times and loaded individually into 0.25 ml French straws. Fourteen adult llamas were used as recipients and embryos were transferred only into the left uterine horn of the recipient regardless of side ovulation. All recipient llamas were pregnancy tested by transrectal ultrasonography at 45 days after transfer. Three (21.5%) pregnant females were detected and delivered three normal healthy male calves at term. To our knowledge, these offspring are the first llamas produced following transfer of refrigerated embryos. In summary, llama embryos can be cooled to 5 °C and maintained in storage for up to 24 h without significant losses of viability.EEA BalcarceFil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Abalos, Marcos. INTA, Estación Experimental Agropecuaria Abra Pampa; ArgentinaFil: Acuña, Francisco Antonio. INTA, Estación Experimental Agropecuaria Abra Pampa; ArgentinaFil: Virgili, Rosana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; ArgentinaFil: Requena, Francisco. Universidad de Córdoba. Facultad de Veterinaria. Campus Universitario Rabanales; EspañaFil: Cancino, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina2018-04-12T14:07:26Z2018-04-12T14:07:26Z2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S092144881530078Xhttp://hdl.handle.net/20.500.12123/22280921-4488https://doi.org/10.1016/j.smallrumres.2015.10.007Small ruminant research 132 : 99-102. (November 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:17Zoai:localhost:20.500.12123/2228instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:17.55INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| spellingShingle |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h Aller Atucha, Juan Florencio Llama Transferencia de Embriones Parto Llamas Embryo Transfer Parturition Nacimiento |
| title_short |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_full |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_fullStr |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_full_unstemmed |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| title_sort |
Birth of live llama (Lama glama) derived from embryo transfer storage at 5 °C for 24 h |
| dc.creator.none.fl_str_mv |
Aller Atucha, Juan Florencio Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina |
| author |
Aller Atucha, Juan Florencio |
| author_facet |
Aller Atucha, Juan Florencio Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina |
| author_role |
author |
| author2 |
Abalos, Marcos Acuña, Francisco Antonio Virgili, Rosana Requena, Francisco Cancino, Andrea Karina |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Llama Transferencia de Embriones Parto Llamas Embryo Transfer Parturition Nacimiento |
| topic |
Llama Transferencia de Embriones Parto Llamas Embryo Transfer Parturition Nacimiento |
| dc.description.none.fl_txt_mv |
The objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20 llamas flushed at Day 7 after mating. Fourteen excellent hatched blastocysts were stored in sterile test tubes containing 1.5 ml of TCM 199-Hepes + 10% cow serum (v/v concentration) + antibiotics and cooled to 5 °C (cooling rate = 0.5 °C/min) and maintained at 5 °C during 24 h. After this, the embryos were warmed on warming plate (38 °C), washed three times and loaded individually into 0.25 ml French straws. Fourteen adult llamas were used as recipients and embryos were transferred only into the left uterine horn of the recipient regardless of side ovulation. All recipient llamas were pregnancy tested by transrectal ultrasonography at 45 days after transfer. Three (21.5%) pregnant females were detected and delivered three normal healthy male calves at term. To our knowledge, these offspring are the first llamas produced following transfer of refrigerated embryos. In summary, llama embryos can be cooled to 5 °C and maintained in storage for up to 24 h without significant losses of viability. EEA Balcarce Fil: Aller Atucha, Juan Florencio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Abalos, Marcos. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Acuña, Francisco Antonio. INTA, Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Virgili, Rosana. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; Argentina Fil: Requena, Francisco. Universidad de Córdoba. Facultad de Veterinaria. Campus Universitario Rabanales; España Fil: Cancino, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina |
| description |
The objective of the present work was to evaluate the efficacy of maintaining llama embryos metabolically inhibited but viable at 5 °C during 24 h. Adult females (n = 20) were used as embryo donors and were superstimulated with eCG. A total 61 embryos were non-surgically collected from 16 of the 20 llamas flushed at Day 7 after mating. Fourteen excellent hatched blastocysts were stored in sterile test tubes containing 1.5 ml of TCM 199-Hepes + 10% cow serum (v/v concentration) + antibiotics and cooled to 5 °C (cooling rate = 0.5 °C/min) and maintained at 5 °C during 24 h. After this, the embryos were warmed on warming plate (38 °C), washed three times and loaded individually into 0.25 ml French straws. Fourteen adult llamas were used as recipients and embryos were transferred only into the left uterine horn of the recipient regardless of side ovulation. All recipient llamas were pregnancy tested by transrectal ultrasonography at 45 days after transfer. Three (21.5%) pregnant females were detected and delivered three normal healthy male calves at term. To our knowledge, these offspring are the first llamas produced following transfer of refrigerated embryos. In summary, llama embryos can be cooled to 5 °C and maintained in storage for up to 24 h without significant losses of viability. |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-11 2018-04-12T14:07:26Z 2018-04-12T14:07:26Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S092144881530078X http://hdl.handle.net/20.500.12123/2228 0921-4488 https://doi.org/10.1016/j.smallrumres.2015.10.007 |
| url |
https://www.sciencedirect.com/science/article/pii/S092144881530078X http://hdl.handle.net/20.500.12123/2228 https://doi.org/10.1016/j.smallrumres.2015.10.007 |
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0921-4488 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/restrictedAccess |
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restrictedAccess |
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application/pdf |
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Small ruminant research 132 : 99-102. (November 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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