Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Meta...

Autores: Schuster, Christina; Ben Gharsa, Haifa; Baró Robaina, Yamilé; Manfrino, Romina Guadalupe; Bobushova, Saikal; Gutierrez, Alejandra Concepción; Lopez Lastra, Claudia Cristina; Leclerque, Andreas
Año de publicación: 2026
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: (1) Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.
EEA Rafaela
Fil: Schuster, Christina. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
Fil: Ben Gharsa, Haifa. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
Fil: Baró Robaina, Yamilé. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
Fil: Baró Robaina, Yamilé. Plant Health Research Institute; Cuba
Fil: Manfrino, Romina Guadalupe. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
Fil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Manfrino, Romina Guadalupe. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Manfrino, Romina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina
Fil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Bobushova, Saikal. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
Fil: Bobushova, Saikal. Kyrgyz-Turkish Manas University. Faculty of Agriculture; Kirguistán
Fil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Gutierrez, Alejandra Concepción. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lopez Lastra, Claudia Cristina. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Leclerque, Andreas. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
Fuente: Journal of Fungi 12 (4) : 272. (April 2026)
Materia: Metarhizium
PCR
Metarhizium anisopliae
Entomogenous Fungi
Genetic Markers
Identification
Hongos Entomopatogenos
Marcadores Genéticos
Identificación
Metarhizium hybridum
Metarhizium parapingshaense
Marcadores Moleculares
Nivel de accesibilidad: acceso abierto
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/25772

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spelling	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaenseSchuster, ChristinaBen Gharsa, HaifaBaró Robaina, YamiléManfrino, Romina GuadalupeBobushova, SaikalGutierrez, Alejandra ConcepciónLopez Lastra, Claudia CristinaLeclerque, AndreasMetarhiziumPCRMetarhizium anisopliaeEntomogenous FungiGenetic MarkersIdentificationHongos EntomopatogenosMarcadores GenéticosIdentificaciónMetarhizium hybridumMetarhizium parapingshaenseMarcadores Moleculares(1) Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.EEA RafaelaFil: Schuster, Christina. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; AlemaniaFil: Ben Gharsa, Haifa. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; AlemaniaFil: Baró Robaina, Yamilé. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; AlemaniaFil: Baró Robaina, Yamilé. Plant Health Research Institute; CubaFil: Manfrino, Romina Guadalupe. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; AlemaniaFil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Manfrino, Romina Guadalupe. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Manfrino, Romina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); ArgentinaFil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Bobushova, Saikal. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; AlemaniaFil: Bobushova, Saikal. Kyrgyz-Turkish Manas University. Faculty of Agriculture; KirguistánFil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Gutierrez, Alejandra Concepción. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lopez Lastra, Claudia Cristina. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Leclerque, Andreas. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; AlemaniaMDPI2026-04-13T11:29:31Z2026-04-13T11:29:31Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25772https://www.mdpi.com/2309-608X/12/4/2722309-608Xhttps://doi.org/10.3390/jof12040272Journal of Fungi 12 (4) : 272. 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dc.title.none.fl_str_mv	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
spellingShingle	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense Schuster, Christina Metarhizium PCR Metarhizium anisopliae Entomogenous Fungi Genetic Markers Identification Hongos Entomopatogenos Marcadores Genéticos Identificación Metarhizium hybridum Metarhizium parapingshaense Marcadores Moleculares
title_short	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_full	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_fullStr	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_full_unstemmed	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
title_sort	Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Metarhizium parapingshaense
dc.creator.none.fl_str_mv	Schuster, Christina Ben Gharsa, Haifa Baró Robaina, Yamilé Manfrino, Romina Guadalupe Bobushova, Saikal Gutierrez, Alejandra Concepción Lopez Lastra, Claudia Cristina Leclerque, Andreas
author	Schuster, Christina
author_facet	Schuster, Christina Ben Gharsa, Haifa Baró Robaina, Yamilé Manfrino, Romina Guadalupe Bobushova, Saikal Gutierrez, Alejandra Concepción Lopez Lastra, Claudia Cristina Leclerque, Andreas
author_role	author
author2	Ben Gharsa, Haifa Baró Robaina, Yamilé Manfrino, Romina Guadalupe Bobushova, Saikal Gutierrez, Alejandra Concepción Lopez Lastra, Claudia Cristina Leclerque, Andreas
author2_role	author author author author author author author
dc.subject.none.fl_str_mv	Metarhizium PCR Metarhizium anisopliae Entomogenous Fungi Genetic Markers Identification Hongos Entomopatogenos Marcadores Genéticos Identificación Metarhizium hybridum Metarhizium parapingshaense Marcadores Moleculares
topic	Metarhizium PCR Metarhizium anisopliae Entomogenous Fungi Genetic Markers Identification Hongos Entomopatogenos Marcadores Genéticos Identificación Metarhizium hybridum Metarhizium parapingshaense Marcadores Moleculares
dc.description.none.fl_txt_mv	(1) Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense. EEA Rafaela Fil: Schuster, Christina. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania Fil: Ben Gharsa, Haifa. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania Fil: Baró Robaina, Yamilé. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania Fil: Baró Robaina, Yamilé. Plant Health Research Institute; Cuba Fil: Manfrino, Romina Guadalupe. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania Fil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Manfrino, Romina Guadalupe. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Manfrino, Romina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IdICaL); Argentina Fil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Bobushova, Saikal. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania Fil: Bobushova, Saikal. Kyrgyz-Turkish Manas University. Faculty of Agriculture; Kirguistán Fil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Gutierrez, Alejandra Concepción. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Lopez Lastra, Claudia Cristina. Universidad Nacional de La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Leclerque, Andreas. Technische Universität Darmstadt. Department of Biology. Insect-Associated Microorganisms and Microbial Control; Alemania
description	(1) Background: Potentially arthropod-pathogenic and plant-associated Metarhizium fungi are of high interest for basic research, biological pest control and plant growth promotion. Unambiguous species delineation enabling the taxonomic assignment of new isolates and the identification of new Metarhizium species is of crucial importance for both research and application. Recently, the new species Metarhizium hybridum and Metarhizium parapingshaense were introduced on the basis of phylogenomic studies. (2) Methods: Neighbor- joining and Bayesian inference-based phylogenetic reconstruction of ribosomal intergenic spacer (rIGS) sequences were used to critically evaluate new species introductions. A species-discriminating diagnostic PCR tool for Metarhizium was adapted to M. hybridum and M. parapingshaense. GenBank database mining was performed to identify cryptic descriptions of the new species. (3) Results: The introduction of M. hybridum and M. parapingshaense was corroborated by rIGS sequence comparison. Data mining revealed cryptic first descriptions of M. hybridum from Canada, China, Colombia, Costa Rica, Cuba, Honduras, Mexico, New Zealand, the USA and the Philippines, and of M. parapingshaense from China, India, Japan, the Philippines and South Korea. (4) Conclusions: Results support the reliability of rIGS as a single taxonomic marker for species-level identification of Metarhizium fungi. Species-discriminating diagnostic PCR was successfully adapted to enable the sequencing-independent identification of the confirmed new species M. hybridum and M. parapingshaense.
publishDate	2026
dc.date.none.fl_str_mv	2026-04-13T11:29:31Z 2026-04-13T11:29:31Z 2026-04
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
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dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/25772 https://www.mdpi.com/2309-608X/12/4/272 2309-608X https://doi.org/10.3390/jof12040272
url	http://hdl.handle.net/20.500.12123/25772 https://www.mdpi.com/2309-608X/12/4/272 https://doi.org/10.3390/jof12040272
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dc.language.none.fl_str_mv	eng
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dc.publisher.none.fl_str_mv	MDPI
publisher.none.fl_str_mv	MDPI
dc.source.none.fl_str_mv	Journal of Fungi 12 (4) : 272. (April 2026) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
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repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Species-Discriminating Diagnostic PCR, Ribosomal Intergenic Spacer-Based Single-Marker Taxonomy and Cryptic Descriptions of the Fungal Entomopathogens Metarhizium hybridum and Meta...

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