Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy

Autores
Schuster, Christina; Baró Robaina, Yamilé; Ben Gharsa, Haifa; Bobushova, Saikal; Manfrino, Romina Guadalupe; Gutierrez, Alejandra Concepción; Lopez Lastra, Claudia Cristina; Doolotkeldieva, Tinatin; Leclerque, Andreas
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
(1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
Fil: Schuster, Christina. Universitat Technische Darmstadt; Alemania
Fil: Baró Robaina, Yamilé. Plant Health Research Institute; Cuba. Universitat Technische Darmstadt; Alemania
Fil: Ben Gharsa, Haifa. Universitat Technische Darmstadt; Alemania
Fil: Bobushova, Saikal. Kyrgyz-Turkish Manas University; Kirguistán
Fil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universitat Technische Darmstadt; Alemania
Fil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Doolotkeldieva, Tinatin. Kyrgyz-Turkish Manas University; Kirguistán
Fil: Leclerque, Andreas. Universitat Technische Darmstadt; Alemania
Materia
DIAGNOSTIC PCR
METARHIZIUM ANISOPLIAE
METARHIZIUM BRUNNEUM
METARHIZIUM ROBERTSII
METARHIZUM PINGHAENSE
PARB CLADE
RIBOSOMAL INTERGENIC SPACER (RIGS)
SINGLE-MARKER PHYLOGENY
SPECIES DELINEATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227256
Acceder
id CONICETDig_ca93095f0879ced848472ff80ab8eb55
oai_identifier_str oai:ri.conicet.gov.ar:11336/227256
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker TaxonomySchuster, ChristinaBaró Robaina, YamiléBen Gharsa, HaifaBobushova, SaikalManfrino, Romina GuadalupeGutierrez, Alejandra ConcepciónLopez Lastra, Claudia CristinaDoolotkeldieva, TinatinLeclerque, AndreasDIAGNOSTIC PCRMETARHIZIUM ANISOPLIAEMETARHIZIUM BRUNNEUMMETARHIZIUM ROBERTSIIMETARHIZUM PINGHAENSEPARB CLADERIBOSOMAL INTERGENIC SPACER (RIGS)SINGLE-MARKER PHYLOGENYSPECIES DELINEATIONhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1(1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.Fil: Schuster, Christina. Universitat Technische Darmstadt; AlemaniaFil: Baró Robaina, Yamilé. Plant Health Research Institute; Cuba. Universitat Technische Darmstadt; AlemaniaFil: Ben Gharsa, Haifa. Universitat Technische Darmstadt; AlemaniaFil: Bobushova, Saikal. Kyrgyz-Turkish Manas University; KirguistánFil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universitat Technische Darmstadt; AlemaniaFil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Doolotkeldieva, Tinatin. Kyrgyz-Turkish Manas University; KirguistánFil: Leclerque, Andreas. Universitat Technische Darmstadt; AlemaniaMultidisciplinary Digital Publishing Institute2023-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227256Schuster, Christina; Baró Robaina, Yamilé; Ben Gharsa, Haifa; Bobushova, Saikal; Manfrino, Romina Guadalupe; et al.; Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 9; 10; 10-2023; 2-162309-608XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/9/10/996info:eu-repo/semantics/altIdentifier/doi/10.3390/jof9100996info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:32Zoai:ri.conicet.gov.ar:11336/227256instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:33.457CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
title Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
spellingShingle Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
Schuster, Christina
DIAGNOSTIC PCR
METARHIZIUM ANISOPLIAE
METARHIZIUM BRUNNEUM
METARHIZIUM ROBERTSII
METARHIZUM PINGHAENSE
PARB CLADE
RIBOSOMAL INTERGENIC SPACER (RIGS)
SINGLE-MARKER PHYLOGENY
SPECIES DELINEATION
title_short Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
title_full Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
title_fullStr Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
title_full_unstemmed Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
title_sort Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy
dc.creator.none.fl_str_mv Schuster, Christina
Baró Robaina, Yamilé
Ben Gharsa, Haifa
Bobushova, Saikal
Manfrino, Romina Guadalupe
Gutierrez, Alejandra Concepción
Lopez Lastra, Claudia Cristina
Doolotkeldieva, Tinatin
Leclerque, Andreas
author Schuster, Christina
author_facet Schuster, Christina
Baró Robaina, Yamilé
Ben Gharsa, Haifa
Bobushova, Saikal
Manfrino, Romina Guadalupe
Gutierrez, Alejandra Concepción
Lopez Lastra, Claudia Cristina
Doolotkeldieva, Tinatin
Leclerque, Andreas
author_role author
author2 Baró Robaina, Yamilé
Ben Gharsa, Haifa
Bobushova, Saikal
Manfrino, Romina Guadalupe
Gutierrez, Alejandra Concepción
Lopez Lastra, Claudia Cristina
Doolotkeldieva, Tinatin
Leclerque, Andreas
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIAGNOSTIC PCR
METARHIZIUM ANISOPLIAE
METARHIZIUM BRUNNEUM
METARHIZIUM ROBERTSII
METARHIZUM PINGHAENSE
PARB CLADE
RIBOSOMAL INTERGENIC SPACER (RIGS)
SINGLE-MARKER PHYLOGENY
SPECIES DELINEATION
topic DIAGNOSTIC PCR
METARHIZIUM ANISOPLIAE
METARHIZIUM BRUNNEUM
METARHIZIUM ROBERTSII
METARHIZUM PINGHAENSE
PARB CLADE
RIBOSOMAL INTERGENIC SPACER (RIGS)
SINGLE-MARKER PHYLOGENY
SPECIES DELINEATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv (1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
Fil: Schuster, Christina. Universitat Technische Darmstadt; Alemania
Fil: Baró Robaina, Yamilé. Plant Health Research Institute; Cuba. Universitat Technische Darmstadt; Alemania
Fil: Ben Gharsa, Haifa. Universitat Technische Darmstadt; Alemania
Fil: Bobushova, Saikal. Kyrgyz-Turkish Manas University; Kirguistán
Fil: Manfrino, Romina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Universitat Technische Darmstadt; Alemania
Fil: Gutierrez, Alejandra Concepción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Lopez Lastra, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Doolotkeldieva, Tinatin. Kyrgyz-Turkish Manas University; Kirguistán
Fil: Leclerque, Andreas. Universitat Technische Darmstadt; Alemania
description (1) Background: The entomopathogenic fungus Metarhizium anisopliae sensu lato forms a species complex, comprising a tight cluster made up of four species, namely M. anisopliae sensu stricto, M. pinghaense, M. robertsii and M. brunneum. Unambiguous species delineation within this “PARB clade” that enables both the taxonomic assignment of new isolates and the identification of potentially new species is highly solicited. (2) Methods: Species-discriminating primer pairs targeting the ribosomal intergenic spacer (rIGS) sequence were designed and a diagnostic PCR protocol established. A partial rIGS sequence, referred to as rIGS-ID800, was introduced as a molecular taxonomic marker for PARB species delineation. (3) Results: PARB species from a validation strain set not implied in primer design were clearly discriminated using the diagnostic PCR protocol developed. Using rIGS-ID800 as a single sequence taxonomic marker gave rise to a higher resolution and statistically better supported delineation of PARB clade species. (4) Conclusions: Reliable species discrimination within the Metarhizium PARB clade is possible through both sequencing-independent diagnostic PCR and sequencing-dependent single marker comparison, both based on the rIGS marker.
publishDate 2023
dc.date.none.fl_str_mv 2023-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227256
Schuster, Christina; Baró Robaina, Yamilé; Ben Gharsa, Haifa; Bobushova, Saikal; Manfrino, Romina Guadalupe; et al.; Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 9; 10; 10-2023; 2-16
2309-608X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227256
identifier_str_mv Schuster, Christina; Baró Robaina, Yamilé; Ben Gharsa, Haifa; Bobushova, Saikal; Manfrino, Romina Guadalupe; et al.; Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy; Multidisciplinary Digital Publishing Institute; Journal of Fungi; 9; 10; 10-2023; 2-16
2309-608X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/9/10/996
info:eu-repo/semantics/altIdentifier/doi/10.3390/jof9100996
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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