Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens

Autores
Pugliese, Bruno Daniel; Rodriguez Pardina, Patricia; Edwards Molina, Juan Pablo; Stenglein, Sebastían Alberto; Pastor, Silvina Estela
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chickpea Yellowing Syndrome (CYS) is a major disease complex affecting chickpea (Cicer arietinum L.) in Argentina, yet its etiology remains poorly understood. This study aimed to identify primary fungal and viral agents associated with CYS. A comprehensive survey (2020–2022) collected symptomatic and asymptomatic plants from 22 fields in the provinces of Córdoba, Santiago del Estero, Catamarca, and Salta. Fungal agents were identified by morphological and molecular methods. The pathogenicity of 55 Fusarium spp. isolates was evaluated on the susceptible cultivar ‘Kiara’, while aggressiveness of pathogenic isolates was characterized by the Area Under the Disease Progress Curve calculated from disease incidence. Viral presence was screened using ELISA and RT-PCR. Fusarium was the dominant fungal genus (74%), with Fusarium oxysporum species complex being the most prevalent. Pathogenicity tests confirmed 82% of the isolates as pathogenic. Notably, pathogenic isolates were also recovered from asymptomatic plants. Aggressiveness analysis classified isolates into distinct clusters, identifying a highly aggressive F. proliferatum isolate. Crucially, this work provides the first report of bean leafroll virus (BLRV; Luteovirus phaseoli) and alfalfa mosaic virus (AMV; Alfamovirus AMV) infecting chickpea in Argentina. BLRV was detected in 36% of fields and AMV in 9%. These findings demonstrate that CYS etiology is complex, resulting from the interaction of Fusarium species and viruses, providing the essential knowledge required to design effective integrated management strategies.
Instituto de Patologìa Vegetal
Fil: Pugliese, Bruno Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Pugliese, Bruno Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Edwards Molina, Juan Pablo. Bayer Crop Science. Estación Experimental Fontezuela; Argentina
Fil: Stenglein, Sebastían Alberto. Universidad Nacional del Centro de La Provincia de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biodiversidad y Biotecnología: Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología: Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
European Journal of Plant Pathology : 1-20. (Published: 22 March 2026)
Materia
Cicer arietinum
Fusarium proliferatum
Bean Leafroll Virus
Alfalfa Mosaic Virus
Chickpeas
Plant Diseases
Argentina
Garbanzo
Enfermedades de las Plantas
Fusarium oxysporum species complex
Yellowing
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogensPugliese, Bruno DanielRodriguez Pardina, PatriciaEdwards Molina, Juan PabloStenglein, Sebastían AlbertoPastor, Silvina EstelaCicer arietinumFusarium proliferatumBean Leafroll VirusAlfalfa Mosaic VirusChickpeasPlant DiseasesArgentinaGarbanzoEnfermedades de las PlantasFusarium oxysporum species complexYellowingChickpea Yellowing Syndrome (CYS) is a major disease complex affecting chickpea (Cicer arietinum L.) in Argentina, yet its etiology remains poorly understood. This study aimed to identify primary fungal and viral agents associated with CYS. A comprehensive survey (2020–2022) collected symptomatic and asymptomatic plants from 22 fields in the provinces of Córdoba, Santiago del Estero, Catamarca, and Salta. Fungal agents were identified by morphological and molecular methods. The pathogenicity of 55 Fusarium spp. isolates was evaluated on the susceptible cultivar ‘Kiara’, while aggressiveness of pathogenic isolates was characterized by the Area Under the Disease Progress Curve calculated from disease incidence. Viral presence was screened using ELISA and RT-PCR. Fusarium was the dominant fungal genus (74%), with Fusarium oxysporum species complex being the most prevalent. Pathogenicity tests confirmed 82% of the isolates as pathogenic. Notably, pathogenic isolates were also recovered from asymptomatic plants. Aggressiveness analysis classified isolates into distinct clusters, identifying a highly aggressive F. proliferatum isolate. Crucially, this work provides the first report of bean leafroll virus (BLRV; Luteovirus phaseoli) and alfalfa mosaic virus (AMV; Alfamovirus AMV) infecting chickpea in Argentina. BLRV was detected in 36% of fields and AMV in 9%. These findings demonstrate that CYS etiology is complex, resulting from the interaction of Fusarium species and viruses, providing the essential knowledge required to design effective integrated management strategies.Instituto de Patologìa VegetalFil: Pugliese, Bruno Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Pugliese, Bruno Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Edwards Molina, Juan Pablo. Bayer Crop Science. Estación Experimental Fontezuela; ArgentinaFil: Stenglein, Sebastían Alberto. Universidad Nacional del Centro de La Provincia de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biodiversidad y Biotecnología: Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología: ArgentinaFil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaSpringer2026-04-08T10:08:14Z2026-04-08T10:08:14Z2026-03-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25707https://link.springer.com/article/10.1007/s10658-026-03215-40929-18731573-8469 (online)https://doi.org/10.1007/s10658-026-03215-4European Journal of Plant Pathology : 1-20. (Published: 22 March 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-29T11:46:32Zoai:localhost:20.500.12123/25707instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-29 11:46:32.923INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
title Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
spellingShingle Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
Pugliese, Bruno Daniel
Cicer arietinum
Fusarium proliferatum
Bean Leafroll Virus
Alfalfa Mosaic Virus
Chickpeas
Plant Diseases
Argentina
Garbanzo
Enfermedades de las Plantas
Fusarium oxysporum species complex
Yellowing
title_short Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
title_full Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
title_fullStr Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
title_full_unstemmed Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
title_sort Etiology of Chickpea Yellowing Syndrome (CYS) in Argentina: the role of fungal and viral pathogens
dc.creator.none.fl_str_mv Pugliese, Bruno Daniel
Rodriguez Pardina, Patricia
Edwards Molina, Juan Pablo
Stenglein, Sebastían Alberto
Pastor, Silvina Estela
author Pugliese, Bruno Daniel
author_facet Pugliese, Bruno Daniel
Rodriguez Pardina, Patricia
Edwards Molina, Juan Pablo
Stenglein, Sebastían Alberto
Pastor, Silvina Estela
author_role author
author2 Rodriguez Pardina, Patricia
Edwards Molina, Juan Pablo
Stenglein, Sebastían Alberto
Pastor, Silvina Estela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cicer arietinum
Fusarium proliferatum
Bean Leafroll Virus
Alfalfa Mosaic Virus
Chickpeas
Plant Diseases
Argentina
Garbanzo
Enfermedades de las Plantas
Fusarium oxysporum species complex
Yellowing
topic Cicer arietinum
Fusarium proliferatum
Bean Leafroll Virus
Alfalfa Mosaic Virus
Chickpeas
Plant Diseases
Argentina
Garbanzo
Enfermedades de las Plantas
Fusarium oxysporum species complex
Yellowing
dc.description.none.fl_txt_mv Chickpea Yellowing Syndrome (CYS) is a major disease complex affecting chickpea (Cicer arietinum L.) in Argentina, yet its etiology remains poorly understood. This study aimed to identify primary fungal and viral agents associated with CYS. A comprehensive survey (2020–2022) collected symptomatic and asymptomatic plants from 22 fields in the provinces of Córdoba, Santiago del Estero, Catamarca, and Salta. Fungal agents were identified by morphological and molecular methods. The pathogenicity of 55 Fusarium spp. isolates was evaluated on the susceptible cultivar ‘Kiara’, while aggressiveness of pathogenic isolates was characterized by the Area Under the Disease Progress Curve calculated from disease incidence. Viral presence was screened using ELISA and RT-PCR. Fusarium was the dominant fungal genus (74%), with Fusarium oxysporum species complex being the most prevalent. Pathogenicity tests confirmed 82% of the isolates as pathogenic. Notably, pathogenic isolates were also recovered from asymptomatic plants. Aggressiveness analysis classified isolates into distinct clusters, identifying a highly aggressive F. proliferatum isolate. Crucially, this work provides the first report of bean leafroll virus (BLRV; Luteovirus phaseoli) and alfalfa mosaic virus (AMV; Alfamovirus AMV) infecting chickpea in Argentina. BLRV was detected in 36% of fields and AMV in 9%. These findings demonstrate that CYS etiology is complex, resulting from the interaction of Fusarium species and viruses, providing the essential knowledge required to design effective integrated management strategies.
Instituto de Patologìa Vegetal
Fil: Pugliese, Bruno Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Pugliese, Bruno Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Edwards Molina, Juan Pablo. Bayer Crop Science. Estación Experimental Fontezuela; Argentina
Fil: Stenglein, Sebastían Alberto. Universidad Nacional del Centro de La Provincia de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biodiversidad y Biotecnología: Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Biodiversidad y Biotecnología: Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Chickpea Yellowing Syndrome (CYS) is a major disease complex affecting chickpea (Cicer arietinum L.) in Argentina, yet its etiology remains poorly understood. This study aimed to identify primary fungal and viral agents associated with CYS. A comprehensive survey (2020–2022) collected symptomatic and asymptomatic plants from 22 fields in the provinces of Córdoba, Santiago del Estero, Catamarca, and Salta. Fungal agents were identified by morphological and molecular methods. The pathogenicity of 55 Fusarium spp. isolates was evaluated on the susceptible cultivar ‘Kiara’, while aggressiveness of pathogenic isolates was characterized by the Area Under the Disease Progress Curve calculated from disease incidence. Viral presence was screened using ELISA and RT-PCR. Fusarium was the dominant fungal genus (74%), with Fusarium oxysporum species complex being the most prevalent. Pathogenicity tests confirmed 82% of the isolates as pathogenic. Notably, pathogenic isolates were also recovered from asymptomatic plants. Aggressiveness analysis classified isolates into distinct clusters, identifying a highly aggressive F. proliferatum isolate. Crucially, this work provides the first report of bean leafroll virus (BLRV; Luteovirus phaseoli) and alfalfa mosaic virus (AMV; Alfamovirus AMV) infecting chickpea in Argentina. BLRV was detected in 36% of fields and AMV in 9%. These findings demonstrate that CYS etiology is complex, resulting from the interaction of Fusarium species and viruses, providing the essential knowledge required to design effective integrated management strategies.
publishDate 2026
dc.date.none.fl_str_mv 2026-04-08T10:08:14Z
2026-04-08T10:08:14Z
2026-03-22
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25707
https://link.springer.com/article/10.1007/s10658-026-03215-4
0929-1873
1573-8469 (online)
https://doi.org/10.1007/s10658-026-03215-4
url http://hdl.handle.net/20.500.12123/25707
https://link.springer.com/article/10.1007/s10658-026-03215-4
https://doi.org/10.1007/s10658-026-03215-4
identifier_str_mv 0929-1873
1573-8469 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv European Journal of Plant Pathology : 1-20. (Published: 22 March 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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