Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle

Autores
Pega, Juan Franco; Bucafusco, Danilo; Di Giacomo, Sebastián; Schammas, Juan Manuel; Malacari, Darío Amilcar; Capozzo, Alejandra Victoria; Arzt, J.; Pérez-Beascoechea, C.; Maradei, E.; Rodríguez, L.L.; Borca, Manuel Victor; Perez Filgueira, Daniel Mariano
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.
Instituto de Virología
Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arzt, J. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Pérez Beascoeachea, C. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; Argentina
Fil: Maradei, E. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; Argentina
Fil: Rodríguez, L. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Borca, Manuel Victor. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Virology 87 (5) : 2489-2495. (March 2013)
Materia
Ganado Bovino
Enfermedades de los Animales
Fiebre Aftosa
Respuesta Inmunológica
Sistema Respiratorio
Cattle
Animal Diseases
Foot and Mouth Disease
Immune Response
Respiratory System
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2535
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2535
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected CattlePega, Juan FrancoBucafusco, DaniloDi Giacomo, SebastiánSchammas, Juan ManuelMalacari, Darío AmilcarCapozzo, Alejandra VictoriaArzt, J.Pérez-Beascoechea, C.Maradei, E.Rodríguez, L.L.Borca, Manuel VictorPerez Filgueira, Daniel MarianoGanado BovinoEnfermedades de los AnimalesFiebre AftosaRespuesta InmunológicaSistema RespiratorioCattleAnimal DiseasesFoot and Mouth DiseaseImmune ResponseRespiratory SystemFoot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.Instituto de VirologíaFil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arzt, J. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Pérez Beascoeachea, C. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; ArgentinaFil: Maradei, E. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; ArgentinaFil: Rodríguez, L. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Borca, Manuel Victor. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-06-01T13:49:23Z2018-06-01T13:49:23Z2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://jvi.asm.org/content/87/5/2489.longhttp://hdl.handle.net/20.500.12123/25350022-538X1098-5514http://dx.doi.org/10.1128/JVI.02879-12Journal of Virology 87 (5) : 2489-2495. (March 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-06T09:39:32Zoai:localhost:20.500.12123/2535instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:39:34.375INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
title Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
spellingShingle Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
Pega, Juan Franco
Ganado Bovino
Enfermedades de los Animales
Fiebre Aftosa
Respuesta Inmunológica
Sistema Respiratorio
Cattle
Animal Diseases
Foot and Mouth Disease
Immune Response
Respiratory System
title_short Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
title_full Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
title_fullStr Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
title_full_unstemmed Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
title_sort Early Adaptive Immune Responses in the Respiratory Tract of Foot-and-Mouth Disease Virus-Infected Cattle
dc.creator.none.fl_str_mv Pega, Juan Franco
Bucafusco, Danilo
Di Giacomo, Sebastián
Schammas, Juan Manuel
Malacari, Darío Amilcar
Capozzo, Alejandra Victoria
Arzt, J.
Pérez-Beascoechea, C.
Maradei, E.
Rodríguez, L.L.
Borca, Manuel Victor
Perez Filgueira, Daniel Mariano
author Pega, Juan Franco
author_facet Pega, Juan Franco
Bucafusco, Danilo
Di Giacomo, Sebastián
Schammas, Juan Manuel
Malacari, Darío Amilcar
Capozzo, Alejandra Victoria
Arzt, J.
Pérez-Beascoechea, C.
Maradei, E.
Rodríguez, L.L.
Borca, Manuel Victor
Perez Filgueira, Daniel Mariano
author_role author
author2 Bucafusco, Danilo
Di Giacomo, Sebastián
Schammas, Juan Manuel
Malacari, Darío Amilcar
Capozzo, Alejandra Victoria
Arzt, J.
Pérez-Beascoechea, C.
Maradei, E.
Rodríguez, L.L.
Borca, Manuel Victor
Perez Filgueira, Daniel Mariano
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Fiebre Aftosa
Respuesta Inmunológica
Sistema Respiratorio
Cattle
Animal Diseases
Foot and Mouth Disease
Immune Response
Respiratory System
topic Ganado Bovino
Enfermedades de los Animales
Fiebre Aftosa
Respuesta Inmunológica
Sistema Respiratorio
Cattle
Animal Diseases
Foot and Mouth Disease
Immune Response
Respiratory System
dc.description.none.fl_txt_mv Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.
Instituto de Virología
Fil: Pega, Juan Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bucafusco, Danilo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Schammas, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arzt, J. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Pérez Beascoeachea, C. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; Argentina
Fil: Maradei, E. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios; Argentina
Fil: Rodríguez, L. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Borca, Manuel Victor. USDA. Agricultural Research Service. Plum Island Animal Disease Center; Estados Unidos
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease (FMD) is a highly contagious viral disease which affects both domestic and wild biungulate species. This acute disease, caused by the FMD virus (FMDV), usually includes an active replication phase in the respiratory tract for up to 72 h postinfection, followed by hematogenous dissemination and vesicular lesions at oral and foot epithelia. The role of the early local adaptive immunity of the host in the outcome of the infection is not well understood. Here we report the kinetics of appearance of FMDV-specific antibody-secreting cells (ASC) in lymphoid organs along the respiratory tract and the spleen in cattle infected by aerosol exposure. While no responses were observed for up to 3 days postinfection (dpi), all animals developed FMDV-ASC in all the lymphoid organs studied at 4 dpi. Tracheobronchial lymph nodes were the most reactive organs at this time, and IgM was the predominant isotype, followed by IgG1. Numbers of FMDV-ASC were further augmented at 5 and 6 dpi, with an increasing prevalence in upper respiratory organs. Systemic antibody responses were slightly delayed compared with the local reaction. Also, IgM was the dominant isotype in serum at 5 dpi, coinciding with a sharp decrease of viral RNA detection in peripheral blood. These results indicate that following aerogenous administration, cattle develop a rapid and vigorous genuine local antibody response throughout the respiratory tract. Time course and isotype profiles indicate the presence of an efficient T cell-independent antibody response which drives the IgM-mediated virus clearance in cattle infected by FMDV aerosol exposure.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
2018-06-01T13:49:23Z
2018-06-01T13:49:23Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://jvi.asm.org/content/87/5/2489.long
http://hdl.handle.net/20.500.12123/2535
0022-538X
1098-5514
http://dx.doi.org/10.1128/JVI.02879-12
url http://jvi.asm.org/content/87/5/2489.long
http://hdl.handle.net/20.500.12123/2535
http://dx.doi.org/10.1128/JVI.02879-12
identifier_str_mv 0022-538X
1098-5514
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Virology 87 (5) : 2489-2495. (March 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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